LOCUS BBa_K1150025 5335 bp DNA linear 26-Dec-2024 DEFINITION uniCAS Red Light Switch Part I - Stimulator ACCESSION BBa_K1150025 VERSION BBa_K1150025.1 FEATURES Location/Qualifiers promoter 1..580 /label=CMV promoter 1..580 /label=BBa_K747096 misc_feature 589..615 /label=HA misc_feature 589..615 /label=BBa_K1150016 CDS 624..644 /label=NLS misc_feature 624..644 /label=BBa_K1150010 CDS 653..4753 /label=dCas9 misc_feature 3167..3169 /label=Nickaseless mutation CDS 653..4753 /label=BBa_K1150000 misc_feature 4762..4770 /label=AGS Linker CDS 4762..4770 /label=BBa_K1150013 CDS 4777..5076 /label=PIF6 CDS 4777..5076 /label=BBa_K1150005 CDS 5085..5105 /label=NLS misc_feature 5085..5105 /label=BBa_K1150010 misc_feature 5114..5335 /label=BGH terminator 5114..5335 /label=BBa_K1150012 ORIGIN 1 atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata 61 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat 121 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga 181 gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc 241 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt 301 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat 361 gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag 421 tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc 481 aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga 541 ggtctatata agcagagctg gtttagtgaa ccgtcagatc tactagagta cccatacgat 601 gttccagatt acgcttacta gagcctgcaa gaaaaagatt gaattactag aggacaagaa 661 gtacagcatc ggcctggcca tcggcaccaa ctctgtgggc tgggccgtga tcaccgacga 721 gtacaaggtg cccagcaaga aattcaaggt gctgggcaac accgaccggc acagcatcaa 781 gaagaacctg atcggagccc tgctgttcga cagcggcgaa acagccgagg ccacccggct 841 gaagagaacc gccagaagaa gatacaccag acggaagaac cggatctgct atctgcaaga 901 gatcttcagc aacgagatgg ccaaggtgga cgacagcttc ttccacagac tggaagagtc 961 cttcctggtg gaagaggata agaagcacga gcggcacccc atcttcggca acatcgtgga 1021 cgaggtggcc taccacgaga agtaccccac catctaccac ctgagaaaga aactggtgga 1081 cagcaccgac aaggccgacc tgcggctgat ctatctggcc ctggcccaca tgatcaagtt 1141 ccggggccac ttcctgatcg agggcgacct gaaccccgac aacagcgacg tggacaagct 1201 gttcatccag ctggtgcaga cctacaacca gctgttcgag gaaaacccca tcaacgccag 1261 cggcgtggac gccaaggcca tcctgtctgc cagactgagc aagagcagac ggctggaaaa 1321 tctgatcgcc cagctgcccg gcgagaagaa gaatggcctg ttcggcaacc tgattgccct 1381 gagcctgggc ctgaccccca acttcaagag caacttcgac ctggccgagg atgccaaact 1441 tcagctgagc aaggacacct acgacgacga cctggacaac ctgctggccc agatcggcga 1501 ccagtacgcc gacctgtttc tggccgccaa gaacctgtcc gacgccatcc tgctgagcga 1561 catcctgaga gtgaacaccg agatcaccaa ggcccccctg agcgcctcta tgatcaagag 1621 atacgacgag caccaccagg acctgaccct gctgaaagct ctcgtgcggc agcagctgcc 1681 tgagaagtac aaagagattt tcttcgacca gagcaagaac ggctacgccg gatacattga 1741 cggcggagcc agccaggaag agttctacaa gttcatcaag cccatcctgg aaaagatgga 1801 cggcaccgag gaactgctcg tgaagctgaa cagagaggac ctgctgcgga agcagcggac 1861 cttcgacaac ggcagcatcc cccaccagat ccacctggga gagctgcacg ccattctgcg 1921 gcggcaggaa gatttttacc cattcctgaa ggacaaccgg gaaaagatcg agaagatcct 1981 gaccttccgc atcccctact acgtgggccc tctggccagg ggaaacagca gattcgcctg 2041 gatgaccaga aagagcgagg aaaccatcac cccctggaac ttcgaggaag tggtggacaa 2101 gggcgcttcc gcccagagct tcatcgagcg gatgaccaac ttcgataaga acctgcccaa 2161 cgagaaggtg ctgcccaagc acagcctgct gtacgagtac ttcaccgtgt ataacgagct 2221 gaccaaagtg aaatacgtga ccgagggaat gagaaagccc gccttcctga gcggcgagca 2281 gaaaaaggcc atcgtggacc tgctgttcaa gaccaaccgg aaagtgaccg tgaagcagct 2341 gaaagaggac tacttcaaga aaatcgagtg cttcgactcc gtggaaatct ccggcgtgga 2401 agatcggttc aacgcctccc tgggcacata ccacgatctg ctgaaaatta tcaaggacaa 2461 ggacttcctg gacaatgagg aaaacgagga cattctggaa gatatcgtgc tgaccctgac 2521 actgtttgag gacagagaga tgatcgagga acggctgaaa acctatgccc acctgttcga 2581 cgacaaagtg atgaagcagc tgaagcggcg gagatacacc ggctggggca ggctgagccg 2641 gaagctgatc aacggcatcc gggacaagca gtccggcaag acaatcctgg atttcctgaa 2701 gtccgacggc ttcgccaaca gaaacttcat gcagctgatc cacgacgaca gcctgacctt 2761 taaagaggac atccagaaag cccaggtgtc cggccagggc gatagcctgc acgagcacat 2821 tgccaatctg gcaggcagcc ccgccattaa gaagggcatc ctgcaaacag tgaaggtggt 2881 ggacgagctc gtgaaagtga tgggacggca caagcccgag aacatcgtga tcgaaatggc 2941 cagagagaac cagaccaccc agaagggaca gaagaacagc cgcgagagaa tgaagcggat 3001 cgaagagggc atcaaagagc tgggcagcca gatcctgaaa gaacaccccg tggaaaacac 3061 ccagcttcag aacgagaagc tgtacctgta ctacctgcaa aatgggcggg atatgtacgt 3121 ggaccaggaa ctggacatca accggctgtc cgactacgat gtggacgcta tcgtgcctca 3181 gagctttctg aaggacgact ccatcgacaa caaggtgctg accagaagcg acaagaaccg 3241 gggcaagagc gacaacgtgc cctccgaaga ggtcgtgaag aagatgaaga actactggcg 3301 gcagctgctg aacgccaagc tgattaccca gagaaagttc gacaatctga ccaaggccga 3361 gagaggcggc ctgagcgaac tggataaggc cggattcatc aagagacagc tggtggaaac 3421 ccggcagatc acaaagcacg tggcacagat cctggactcc cggatgaaca ctaagtacga 3481 cgagaatgac aagctgatcc gggaagtgaa agtgatcacc ctgaagtcca agctggtgtc 3541 cgatttccgg aaggatttcc agttttacaa agtgcgcgag atcaacaact accaccacgc 3601 ccacgacgcc tacctgaacg ccgtcgtggg aaccgccctg atcaaaaagt accctaagct 3661 ggaaagcgag ttcgtgtacg gcgactacaa ggtgtacgac gtgcggaaga tgatcgccaa 3721 gagcgagcag gaaatcggca aggctaccgc caagtacttc ttctacagca acatcatgaa 3781 ctttttcaag accgagatta ccctggccaa cggcgagatc cggaagcggc ctctgatcga 3841 gacaaacggc gaaaccgggg agatcgtgtg ggataagggc cgggattttg ccaccgtgcg 3901 gaaagtgctg agcatgcccc aagtgaatat cgtgaaaaag accgaggtgc agacaggcgg 3961 cttcagcaaa gagtctatcc tgcccaagag gaacagcgat aagctgatcg ccagaaagaa 4021 ggactgggac cctaagaagt acggcggctt cgacagcccc accgtggcct attctgtgct 4081 ggtggtggcc aaagtggaaa agggcaagtc caagaaactg aagagtgtga aagagctgct 4141 ggggatcacc atcatggaaa gaagcagctt cgagaagaat cccatcgact ttctggaagc 4201 caagggctac aaagaagtga aaaaggacct gatcatcaag ctgcctaagt actccctgtt 4261 cgagctggaa aacggccgga agagaatgct ggcctctgcg ggcgaactgc aaaagggaaa 4321 cgaactggcc ctgccctcca aatatgtgaa cttcctgtac ctggccagcc actatgagaa 4381 gctgaagggc tcccccgagg ataatgagca gaaacagctg tttgtggaac agcacaagca 4441 ctacctggac gagatcatcg agcagatcag cgagttctcc aagagagtga tcctggccga 4501 cgctaatctg gacaaagtgc tgtccgccta caacaagcac cgggataagc ccatcagaga 4561 gcaggccgag aatatcatcc acctgtttac cctgaccaat ctgggagccc ctgccgcctt 4621 caagtacttt gacaccacca tcgaccggaa gaggtacacc agcaccaaag aggtgctgga 4681 cgccaccctg atccaccaga gcatcaccgg cctgtacgag acacggatcg acctgtctca 4741 gctgggaggc gactactaga ggcaggctcc tactagatga tgttcttacc aaccgattat 4801 tgttgcaggt taagcgatca agagtatatg gagcttgtgt ttgagaatgg ccagattctt 4861 gcaaagggcc aaagatccaa cgtttctctg cataatcaac gtaccaaatc gatcatggat 4921 ttgtatgagg cagagtataa cgaggatttc atgaagagta tcatccatgg tggtggtggt 4981 gccatcacaa atctcgggga cacgcaggtt gttccacaaa gtcatgttgc tgctgcccat 5041 gaaacaaaca tgttggaaag caataaacat gttgactact agagcctgca agaaaaagat 5101 tgaattacta gaggtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc 5161 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc 5221 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa 5281 gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct ctatg //