BBa_K1150000 1 BBa_K1150000 dCas9 2013-09-14T11:00:00Z 2015-05-08T01:09:27Z Streptococcus pyogenes, Zhang lab The human codon optimized Cas9 from the organism Streptococcus pyogenes was standardized (RFC 25). Additionally we mutated the Nickase function. In the end we engineered a DNA binding protein, that can be fused with different effectors in order to regulate gene expression. false false _1462_ 0 16627 9 It's complicated false Restriction sites were deleted by primer overhangs. Parts were fused together by fusion PCR. The nickase function was mutated in order to abbolish the demolition of DNA. false Freiburg 2013 annotation2347618 1 dCas9 range2347618 1 1 4101 annotation2354601 1 Nickaseless mutation range2354601 1 2515 2517 BBa_K1150016 1 BBa_K1150016 HA tag 2013-09-15T11:00:00Z 2015-05-08T01:09:27Z ... .. false false _1462_ 0 16627 9 In stock false .. false Freiburg 2013 annotation2347937 1 HA range2347937 1 1 27 BBa_K1150029 1 BBa_K1150029 uniCAS UV Light Switch Part I - Stimulator 2013-09-16T11:00:00Z 2015-05-08T01:09:27Z .. .. false false _1462_ 0 16627 9 It's complicated false .. false Freiburg 2013 component2354497 1 BBa_K1150016 component2354503 1 BBa_K1150013 component2354499 1 BBa_K1150010 component2354495 1 BBa_K747096 component2354501 1 BBa_K1150000 component2354509 1 BBa_K1150012 component2354507 1 BBa_K1150010 component2354505 1 BBa_K1150006 annotation2354501 1 BBa_K1150000 range2354501 1 653 4753 annotation2354499 1 BBa_K1150010 range2354499 1 624 644 annotation2354495 1 BBa_K747096 range2354495 1 1 580 annotation2354509 1 BBa_K1150012 range2354509 1 5926 6147 annotation2354507 1 BBa_K1150010 range2354507 1 5897 5917 annotation2354503 1 BBa_K1150013 range2354503 1 4762 4770 annotation2354497 1 BBa_K1150016 range2354497 1 589 615 annotation2354505 1 BBa_K1150006 range2354505 1 4779 5888 BBa_K747096 1 BBa_K747096 CMV-promoter 2012-09-25T11:00:00Z 2015-05-08T01:13:11Z not sure Released HQ 2013 follows false false _998_ 0 13224 9 In stock false c false Lucas Schneider annotation2199147 1 CMV range2199147 1 1 580 BBa_K1150012 1 BBa_K1150012 BGH terminator 2013-09-15T11:00:00Z 2015-05-08T01:09:27Z .. .. false false _1462_ 0 16627 9 In stock false .. false Freiburg 2013 annotation2347917 1 BGH range2347917 1 1 222 BBa_K1150010 1 BBa_K1150010 NLS 2013-09-15T11:00:00Z 2015-05-08T01:09:27Z .. This NLS.. false false _1462_ 0 16627 9 In stock false .. false Freiburg 2013 annotation2347465 1 NLS range2347465 1 1 21 BBa_K1150013 1 BBa_K1150013 AGS Linker 2013-09-15T11:00:00Z 2015-05-08T01:09:27Z ... ... false false _1462_ 0 16627 9 In stock false ... false Freiburg 2013 annotation2347934 1 AGS Linker range2347934 1 1 9 BBa_K1150006 1 BBa_K1150006 UVR8 2013-09-15T11:00:00Z 2015-05-08T01:09:27Z ... UVR8 ... false false _1462_ 0 16627 9 In stock false ... false Freiburg 2013 annotation2347612 1 UVR8 range2347612 1 1 1110 BBa_K1150000_sequence 1 gacaagaagtacagcatcggcctggccatcggcaccaactctgtgggctgggccgtgatcaccgacgagtacaaggtgcccagcaagaaattcaaggtgctgggcaacaccgaccggcacagcatcaagaagaacctgatcggagccctgctgttcgacagcggcgaaacagccgaggccacccggctgaagagaaccgccagaagaagatacaccagacggaagaaccggatctgctatctgcaagagatcttcagcaacgagatggccaaggtggacgacagcttcttccacagactggaagagtccttcctggtggaagaggataagaagcacgagcggcaccccatcttcggcaacatcgtggacgaggtggcctaccacgagaagtaccccaccatctaccacctgagaaagaaactggtggacagcaccgacaaggccgacctgcggctgatctatctggccctggcccacatgatcaagttccggggccacttcctgatcgagggcgacctgaaccccgacaacagcgacgtggacaagctgttcatccagctggtgcagacctacaaccagctgttcgaggaaaaccccatcaacgccagcggcgtggacgccaaggccatcctgtctgccagactgagcaagagcagacggctggaaaatctgatcgcccagctgcccggcgagaagaagaatggcctgttcggcaacctgattgccctgagcctgggcctgacccccaacttcaagagcaacttcgacctggccgaggatgccaaacttcagctgagcaaggacacctacgacgacgacctggacaacctgctggcccagatcggcgaccagtacgccgacctgtttctggccgccaagaacctgtccgacgccatcctgctgagcgacatcctgagagtgaacaccgagatcaccaaggcccccctgagcgcctctatgatcaagagatacgacgagcaccaccaggacctgaccctgctgaaagctctcgtgcggcagcagctgcctgagaagtacaaagagattttcttcgaccagagcaagaacggctacgccggatacattgacggcggagccagccaggaagagttctacaagttcatcaagcccatcctggaaaagatggacggcaccgaggaactgctcgtgaagctgaacagagaggacctgctgcggaagcagcggaccttcgacaacggcagcatcccccaccagatccacctgggagagctgcacgccattctgcggcggcaggaagatttttacccattcctgaaggacaaccgggaaaagatcgagaagatcctgaccttccgcatcccctactacgtgggccctctggccaggggaaacagcagattcgcctggatgaccagaaagagcgaggaaaccatcaccccctggaacttcgaggaagtggtggacaagggcgcttccgcccagagcttcatcgagcggatgaccaacttcgataagaacctgcccaacgagaaggtgctgcccaagcacagcctgctgtacgagtacttcaccgtgtataacgagctgaccaaagtgaaatacgtgaccgagggaatgagaaagcccgccttcctgagcggcgagcagaaaaaggccatcgtggacctgctgttcaagaccaaccggaaagtgaccgtgaagcagctgaaagaggactacttcaagaaaatcgagtgcttcgactccgtggaaatctccggcgtggaagatcggttcaacgcctccctgggcacataccacgatctgctgaaaattatcaaggacaaggacttcctggacaatgaggaaaacgaggacattctggaagatatcgtgctgaccctgacactgtttgaggacagagagatgatcgaggaacggctgaaaacctatgcccacctgttcgacgacaaagtgatgaagcagctgaagcggcggagatacaccggctggggcaggctgagccggaagctgatcaacggcatccgggacaagcagtccggcaagacaatcctggatttcctgaagtccgacggcttcgccaacagaaacttcatgcagctgatccacgacgacagcctgacctttaaagaggacatccagaaagcccaggtgtccggccagggcgatagcctgcacgagcacattgccaatctggcaggcagccccgccattaagaagggcatcctgcaaacagtgaaggtggtggacgagctcgtgaaagtgatgggacggcacaagcccgagaacatcgtgatcgaaatggccagagagaaccagaccacccagaagggacagaagaacagccgcgagagaatgaagcggatcgaagagggcatcaaagagctgggcagccagatcctgaaagaacaccccgtggaaaacacccagcttcagaacgagaagctgtacctgtactacctgcaaaatgggcgggatatgtacgtggaccaggaactggacatcaaccggctgtccgactacgatgtggacgctatcgtgcctcagagctttctgaaggacgactccatcgacaacaaggtgctgaccagaagcgacaagaaccggggcaagagcgacaacgtgccctccgaagaggtcgtgaagaagatgaagaactactggcggcagctgctgaacgccaagctgattacccagagaaagttcgacaatctgaccaaggccgagagaggcggcctgagcgaactggataaggccggattcatcaagagacagctggtggaaacccggcagatcacaaagcacgtggcacagatcctggactcccggatgaacactaagtacgacgagaatgacaagctgatccgggaagtgaaagtgatcaccctgaagtccaagctggtgtccgatttccggaaggatttccagttttacaaagtgcgcgagatcaacaactaccaccacgcccacgacgcctacctgaacgccgtcgtgggaaccgccctgatcaaaaagtaccctaagctggaaagcgagttcgtgtacggcgactacaaggtgtacgacgtgcggaagatgatcgccaagagcgagcaggaaatcggcaaggctaccgccaagtacttcttctacagcaacatcatgaactttttcaagaccgagattaccctggccaacggcgagatccggaagcggcctctgatcgagacaaacggcgaaaccggggagatcgtgtgggataagggccgggattttgccaccgtgcggaaagtgctgagcatgccccaagtgaatatcgtgaaaaagaccgaggtgcagacaggcggcttcagcaaagagtctatcctgcccaagaggaacagcgataagctgatcgccagaaagaaggactgggaccctaagaagtacggcggcttcgacagccccaccgtggcctattctgtgctggtggtggccaaagtggaaaagggcaagtccaagaaactgaagagtgtgaaagagctgctggggatcaccatcatggaaagaagcagcttcgagaagaatcccatcgactttctggaagccaagggctacaaagaagtgaaaaaggacctgatcatcaagctgcctaagtactccctgttcgagctggaaaacggccggaagagaatgctggcctctgcgggcgaactgcaaaagggaaacgaactggccctgccctccaaatatgtgaacttcctgtacctggccagccactatgagaagctgaagggctcccccgaggataatgagcagaaacagctgtttgtggaacagcacaagcactacctggacgagatcatcgagcagatcagcgagttctccaagagagtgatcctggccgacgctaatctggacaaagtgctgtccgcctacaacaagcaccgggataagcccatcagagagcaggccgagaatatcatccacctgtttaccctgaccaatctgggagcccctgccgccttcaagtactttgacaccaccatcgaccggaagaggtacaccagcaccaaagaggtgctggacgccaccctgatccaccagagcatcaccggcctgtacgagacacggatcgacctgtctcagctgggaggcgac BBa_K1150012_sequence 1 gtgccttctagttgccagccatctgttgtttgcccctcccccgtgccttccttgaccctggaaggtgccactcccactgtcctttcctaataaaatgaggaaattgcatcgcattgtctgagtaggtgtcattctattctggggggtggggtggggcaggacagcaagggggaggattgggaagacaatagcaggcatgctggggatgcggtgggctctatg BBa_K747096_sequence 1 atagtaatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgccaatagggactttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggcagtacatctacgtattagtcatcgctattaccatggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaacaactccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagcagagctggtttagtgaaccgtcagatc BBa_K1150010_sequence 1 cctgcaagaaaaagattgaat BBa_K1150029_sequence 1 atagtaatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgccaatagggactttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggcagtacatctacgtattagtcatcgctattaccatggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaacaactccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagcagagctggtttagtgaaccgtcagatctactagagtacccatacgatgttccagattacgcttactagagcctgcaagaaaaagattgaattactagaggacaagaagtacagcatcggcctggccatcggcaccaactctgtgggctgggccgtgatcaccgacgagtacaaggtgcccagcaagaaattcaaggtgctgggcaacaccgaccggcacagcatcaagaagaacctgatcggagccctgctgttcgacagcggcgaaacagccgaggccacccggctgaagagaaccgccagaagaagatacaccagacggaagaaccggatctgctatctgcaagagatcttcagcaacgagatggccaaggtggacgacagcttcttccacagactggaagagtccttcctggtggaagaggataagaagcacgagcggcaccccatcttcggcaacatcgtggacgaggtggcctaccacgagaagtaccccaccatctaccacctgagaaagaaactggtggacagcaccgacaaggccgacctgcggctgatctatctggccctggcccacatgatcaagttccggggccacttcctgatcgagggcgacctgaaccccgacaacagcgacgtggacaagctgttcatccagctggtgcagacctacaaccagctgttcgaggaaaaccccatcaacgccagcggcgtggacgccaaggccatcctgtctgccagactgagcaagagcagacggctggaaaatctgatcgcccagctgcccggcgagaagaagaatggcctgttcggcaacctgattgccctgagcctgggcctgacccccaacttcaagagcaacttcgacctggccgaggatgccaaacttcagctgagcaaggacacctacgacgacgacctggacaacctgctggcccagatcggcgaccagtacgccgacctgtttctggccgccaagaacctgtccgacgccatcctgctgagcgacatcctgagagtgaacaccgagatcaccaaggcccccctgagcgcctctatgatcaagagatacgacgagcaccaccaggacctgaccctgctgaaagctctcgtgcggcagcagctgcctgagaagtacaaagagattttcttcgaccagagcaagaacggctacgccggatacattgacggcggagccagccaggaagagttctacaagttcatcaagcccatcctggaaaagatggacggcaccgaggaactgctcgtgaagctgaacagagaggacctgctgcggaagcagcggaccttcgacaacggcagcatcccccaccagatccacctgggagagctgcacgccattctgcggcggcaggaagatttttacccattcctgaaggacaaccgggaaaagatcgagaagatcctgaccttccgcatcccctactacgtgggccctctggccaggggaaacagcagattcgcctggatgaccagaaagagcgaggaaaccatcaccccctggaacttcgaggaagtggtggacaagggcgcttccgcccagagcttcatcgagcggatgaccaacttcgataagaacctgcccaacgagaaggtgctgcccaagcacagcctgctgtacgagtacttcaccgtgtataacgagctgaccaaagtgaaatacgtgaccgagggaatgagaaagcccgccttcctgagcggcgagcagaaaaaggccatcgtggacctgctgttcaagaccaaccggaaagtgaccgtgaagcagctgaaagaggactacttcaagaaaatcgagtgcttcgactccgtggaaatctccggcgtggaagatcggttcaacgcctccctgggcacataccacgatctgctgaaaattatcaaggacaaggacttcctggacaatgaggaaaacgaggacattctggaagatatcgtgctgaccctgacactgtttgaggacagagagatgatcgaggaacggctgaaaacctatgcccacctgttcgacgacaaagtgatgaagcagctgaagcggcggagatacaccggctggggcaggctgagccggaagctgatcaacggcatccgggacaagcagtccggcaagacaatcctggatttcctgaagtccgacggcttcgccaacagaaacttcatgcagctgatccacgacgacagcctgacctttaaagaggacatccagaaagcccaggtgtccggccagggcgatagcctgcacgagcacattgccaatctggcaggcagccccgccattaagaagggcatcctgcaaacagtgaaggtggtggacgagctcgtgaaagtgatgggacggcacaagcccgagaacatcgtgatcgaaatggccagagagaaccagaccacccagaagggacagaagaacagccgcgagagaatgaagcggatcgaagagggcatcaaagagctgggcagccagatcctgaaagaacaccccgtggaaaacacccagcttcagaacgagaagctgtacctgtactacctgcaaaatgggcgggatatgtacgtggaccaggaactggacatcaaccggctgtccgactacgatgtggacgctatcgtgcctcagagctttctgaaggacgactccatcgacaacaaggtgctgaccagaagcgacaagaaccggggcaagagcgacaacgtgccctccgaagaggtcgtgaagaagatgaagaactactggcggcagctgctgaacgccaagctgattacccagagaaagttcgacaatctgaccaaggccgagagaggcggcctgagcgaactggataaggccggattcatcaagagacagctggtggaaacccggcagatcacaaagcacgtggcacagatcctggactcccggatgaacactaagtacgacgagaatgacaagctgatccgggaagtgaaagtgatcaccctgaagtccaagctggtgtccgatttccggaaggatttccagttttacaaagtgcgcgagatcaacaactaccaccacgcccacgacgcctacctgaacgccgtcgtgggaaccgccctgatcaaaaagtaccctaagctggaaagcgagttcgtgtacggcgactacaaggtgtacgacgtgcggaagatgatcgccaagagcgagcaggaaatcggcaaggctaccgccaagtacttcttctacagcaacatcatgaactttttcaagaccgagattaccctggccaacggcgagatccggaagcggcctctgatcgagacaaacggcgaaaccggggagatcgtgtgggataagggccgggattttgccaccgtgcggaaagtgctgagcatgccccaagtgaatatcgtgaaaaagaccgaggtgcagacaggcggcttcagcaaagagtctatcctgcccaagaggaacagcgataagctgatcgccagaaagaaggactgggaccctaagaagtacggcggcttcgacagccccaccgtggcctattctgtgctggtggtggccaaagtggaaaagggcaagtccaagaaactgaagagtgtgaaagagctgctggggatcaccatcatggaaagaagcagcttcgagaagaatcccatcgactttctggaagccaagggctacaaagaagtgaaaaaggacctgatcatcaagctgcctaagtactccctgttcgagctggaaaacggccggaagagaatgctggcctctgcgggcgaactgcaaaagggaaacgaactggccctgccctccaaatatgtgaacttcctgtacctggccagccactatgagaagctgaagggctcccccgaggataatgagcagaaacagctgtttgtggaacagcacaagcactacctggacgagatcatcgagcagatcagcgagttctccaagagagtgatcctggccgacgctaatctggacaaagtgctgtccgcctacaacaagcaccgggataagcccatcagagagcaggccgagaatatcatccacctgtttaccctgaccaatctgggagcccctgccgccttcaagtactttgacaccaccatcgaccggaagaggtacaccagcaccaaagaggtgctggacgccaccctgatccaccagagcatcaccggcctgtacgagacacggatcgacctgtctcagctgggaggcgactactagaggcaggctcctactagaggctcctcctcgtaaggttcttatcatctccgctggtgctagccactccgtcgctcttctctctggtgacattgtttgttcttggggtcgaggagaggatggacagttaggtcatggcgatgcagaggatcgaccttctccgactcagcttagcgctttagatggccaccaaattgtttccgttacctgtggtgctgatcacactgttgcttattcacaatcaggcatggaagtctacagttggggatggggtgattttgggagattaggccatggtaactcaagcgacttgtttactccgctaccaatcaaagcattgcacggtattcggatcaagcagattgcttgtggggatagtcattgtttggctgtcactatggaaggagaggtccagagttggggccgcaaccagaatggtcaacttggtctgggggacaccgaagattctctagtgcctcagaagattcaagcctttgagggaatacgaatcaaaatggttgctgctggtgcagaacacactgctgccgttacagaagatggtgacctctatggatggggctggggaagatacggaaatttgggattaggtgaccggactgaccgcttagttcctgaaagagttacctctactggtggtgagaaaatgtcaatggttgcttgtggatggcggcacacaatatcagtttcctactctggagcattgtatacttatggatggagcaaatatggacagctaggacatggagacttggaggatcaccttattcctcacaaactggaagcactgagcaacagttttatctcccagatttcgggaggttggagacatacaatggcattgacttcagatggaaaactatatggatggggttggaataagtttggacaagtaggagtcggcaataatttagatcagtgttctcctgtgcaagtgcgatttcccgatgatcagaaagtagttcaagtctcatgtggatggagacataccttggctgtcactgaaagaaataacgtgtttgcttggggtagaggtacaaatggacagctcggcattggagagtcggttgacaggaactttcccaagattatagaggcactcagcgtcgatggatactagagcctgcaagaaaaagattgaattactagaggtgccttctagttgccagccatctgttgtttgcccctcccccgtgccttccttgaccctggaaggtgccactcccactgtcctttcctaataaaatgaggaaattgcatcgcattgtctgagtaggtgtcattctattctggggggtggggtggggcaggacagcaagggggaggattgggaagacaatagcaggcatgctggggatgcggtgggctctatg BBa_K1150013_sequence 1 gcaggctcc BBa_K1150006_sequence 1 gctcctcctcgtaaggttcttatcatctccgctggtgctagccactccgtcgctcttctctctggtgacattgtttgttcttggggtcgaggagaggatggacagttaggtcatggcgatgcagaggatcgaccttctccgactcagcttagcgctttagatggccaccaaattgtttccgttacctgtggtgctgatcacactgttgcttattcacaatcaggcatggaagtctacagttggggatggggtgattttgggagattaggccatggtaactcaagcgacttgtttactccgctaccaatcaaagcattgcacggtattcggatcaagcagattgcttgtggggatagtcattgtttggctgtcactatggaaggagaggtccagagttggggccgcaaccagaatggtcaacttggtctgggggacaccgaagattctctagtgcctcagaagattcaagcctttgagggaatacgaatcaaaatggttgctgctggtgcagaacacactgctgccgttacagaagatggtgacctctatggatggggctggggaagatacggaaatttgggattaggtgaccggactgaccgcttagttcctgaaagagttacctctactggtggtgagaaaatgtcaatggttgcttgtggatggcggcacacaatatcagtttcctactctggagcattgtatacttatggatggagcaaatatggacagctaggacatggagacttggaggatcaccttattcctcacaaactggaagcactgagcaacagttttatctcccagatttcgggaggttggagacatacaatggcattgacttcagatggaaaactatatggatggggttggaataagtttggacaagtaggagtcggcaataatttagatcagtgttctcctgtgcaagtgcgatttcccgatgatcagaaagtagttcaagtctcatgtggatggagacataccttggctgtcactgaaagaaataacgtgtttgcttggggtagaggtacaaatggacagctcggcattggagagtcggttgacaggaactttcccaagattatagaggcactcagcgtcgatgga BBa_K1150016_sequence 1 tacccatacgatgttccagattacgct igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z