LOCUS       BBa_K1150033            1711 bp    DNA     linear       22-Nov-2024
DEFINITION  Constitutive GFP Reporter
ACCESSION   BBa_K1150033
VERSION     BBa_K1150033.1  
FEATURES             Location/Qualifiers
     misc_feature    6..11
                     /label=NheI
     promoter        12..600
                     /label=CMV promoter
     misc_feature    635..640
                     /label=SacII
     CDS             649..1360
                     /label=acGFP
     misc_feature    1370..1375
                     /label=SalI
     misc_feature    1381..1386
                     /label=BamHI
     misc_feature    1388..1419
                     /label=NLS
     polyA_site      1429..1659
                     /label=bGH_PA terminator
     misc_feature    1698..1701
                     /label=HindIII
     misc_feature    1704..1709
                     /label=KpnI
ORIGIN
        1 agcaggctag ctagttatta atagtaatca attacggggt cattagttca tagcccatat
       61 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
      121 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
      181 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg
      241 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
      301 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc
      361 atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt
      421 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac
      481 caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc
      541 ggtaggcgtg tacggtggga ggtctatata agcagagctg gtttagtgaa ccgtcagatc
      601 agccggagca atggcagtcc ggtagcgctg gttccgcgga ccgctcgatg gtgagcaagg
      661 gcgccgagct gttcaccggc atcgtgccca tcctgatcga gctgaatggc gatgtgaatg
      721 gccacaagtt cagcgtgagc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
      781 tgaagttcat ctgcaccacc ggcaagctgc ctgtgccctg gcccaccctg gtgaccaccc
      841 tgagctacgg cgtgcagtgc ttctcacgct accccgatca catgaagcag cacgacttct
      901 tcaagagcgc catgcctgag ggctacatcc aggagcgcac catcttcttc gaggatgacg
      961 gcaactacaa gtcgcgcgcc gaggtgaagt tcgagggcga taccctggtg aatcgcatcg
     1021 agctgaccgg caccgatttc aaggaggatg gcaacatcct gggcaataag atggagtaca
     1081 actacaacgc ccacaatgtg tacatcatga ccgacaaggc caagaatggc atcaaggtga
     1141 acttcaagat ccgccacaac atcgaggatg gcagcgtgca gctggccgac cactaccagc
     1201 agaatacccc catcggcgat ggccctgtgc tgctgcccga taaccactac ctgtccaccc
     1261 agagcgccct gtccaaggac cccaacgaga agcgcgatca catgatctac ttcggcttcg
     1321 tgaccgccgc cgccatcacc cacggcatgg acgagctgta caagggcgtc gacgggtcgg
     1381 atccctcgcc gaagaaaaag cgcaaggtcg aagcgtccta aagatctcga ctgtgccttc
     1441 tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc
     1501 cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg
     1561 tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa
     1621 tagcaggcat gctggggatg cggtgggctc tatggtgcat tagacccatt agcaatacgc
     1681 atgcattcag agctaaagct tacggtacct t
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