BBa_K115004
1
BBa_K115004
A temperature sensitive 5
2008-07-15T11:00:00Z
2015-05-08T01:09:27Z
The sequence is based on the paper "RNA Quadruplex-Based Modulation of Gene
Expression" by Markus Wieland and Jorg S. Hartig.
This part is designed as a temperature inducible RBS. Only designed so far.
true
false
_223_
0
3006
9
Discontinued
false
The secondary structure is important to the function of these regions, but part of the wt secondary structure is destroyed by the scar. We've tried to alter the sequence so the predicted structure (through mfold and those kind of servers) is sort of conserved, but temperature sensitivity still has to be tested. If it doesn't work, possible solution might be the addition of a larger conserved part of the wt, which implies a small part of wt protein sequence as well.
false
Bastiaan van den Berg
annotation1967317
1
His-Tag
range1967317
1
42
71
annotation1967316
1
start
range1967316
1
36
38
annotation1967315
1
SD
range1967315
1
22
28
BBa_K115004_sequence
1
aataattttgtttaagtgtgggaaggagggtgtgcatgggccatcatcatcatcatcatcatcatcatcacagcagcggccatatcgaaggt
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z