LOCUS BBa_K1150048 4706 bp DNA linear 25-Nov-2024 DEFINITION Truncated CMV dCas9 Device #2 ACCESSION BBa_K1150048 VERSION BBa_K1150048.1 FEATURES Location/Qualifiers promoter 1..580 /label=CMV promoter 1..580 /label=BBa_K747096 misc_feature 589..615 /label=HA misc_feature 589..615 /label=BBa_K1150016 CDS 624..644 /label=NLS misc_feature 624..644 /label=BBa_K1150010 CDS 653..4447 /label=BBa_K1150065 CDS 4456..4476 /label=NLS misc_feature 4456..4476 /label=BBa_K1150010 misc_feature 4485..4706 /label=BGH terminator 4485..4706 /label=BBa_K1150012 ORIGIN 1 atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata 61 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat 121 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga 181 gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc 241 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt 301 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat 361 gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag 421 tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc 481 aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga 541 ggtctatata agcagagctg gtttagtgaa ccgtcagatc tactagagta cccatacgat 601 gttccagatt acgcttacta gagcctgcaa gaaaaagatt gaattactag aggacaagaa 661 gtacagcatc ggcctggcca tcggcaccaa ctctgtgggc tgggccgtga tcaccgacga 721 gtacaaggtg cccagcaaga aattcaaggt gctgggcaac accgaccggc acagcatcaa 781 gaagaacctg atcggagccc tgctgttcga cagcggcgaa acagccgagg ccacccggct 841 gaagagaacc gccagaagaa gatacaccag acggaagaac cggatctgct atctgcaaga 901 gatcttcagc aacgagatgg ccaaggtgga cgacagcttc ttccacagac tggaagagtc 961 cttcctggtg gaagaggata agaagcacga gcggcacccc atcttcggca acatcgtgga 1021 cgaggtggcc taccacgaga agtaccccac catctaccac ctgagaaaga aactggtgga 1081 cagcaccgac aaggccgacc tgcggctgat ctatctggcc ctggcccaca tgatcaagtt 1141 ccggggcgac acctacgacg acgacctgga caacctgctg gcccagatcg gcgaccagta 1201 cgccgacctg tttctggccg ccaagaacct gtccgacgcc atcctgctga gcgacatcct 1261 gagagtgaac accgagatca ccaaggcccc cctgagcgcc tctatgatca agagatacga 1321 cgagcaccac caggacctga ccctgctgaa agctctcgtg cggcagcagc tgcctgagaa 1381 gtacaaagag attttcttcg accagagcaa gaacggctac gccggataca ttgacggcgg 1441 agccagccag gaagagttct acaagttcat caagcccatc ctggaaaaga tggacggcac 1501 cgaggaactg ctcgtgaagc tgaacagaga ggacctgctg cggaagcagc ggaccttcga 1561 caacggcagc atcccccacc agatccacct gggagagctg cacgccattc tgcggcggca 1621 ggaagatttt tacccattcc tgaaggacaa ccgggaaaag atcgagaaga tcctgacctt 1681 ccgcatcccc tactacgtgg gccctctggc caggggaaac agcagattcg cctggatgac 1741 cagaaagagc gaggaaacca tcaccccctg gaacttcgag gaagtggtgg acaagggcgc 1801 ttccgcccag agcttcatcg agcggatgac caacttcgat aagaacctgc ccaacgagaa 1861 ggtgctgccc aagcacagcc tgctgtacga gtacttcacc gtgtataacg agctgaccaa 1921 agtgaaatac gtgaccgagg gaatgagaaa gcccgccttc ctgagcggcg agcagaaaaa 1981 ggccatcgtg gacctgctgt tcaagaccaa ccggaaagtg accgtgaagc agctgaaaga 2041 ggactacttc aagaaaatcg agtgcttcga ctccgtggaa atctccggcg tggaagatcg 2101 gttcaacgcc tccctgggca cataccacga tctgctgaaa attatcaagg acaaggactt 2161 cctggacaat gaggaaaacg aggacattct ggaagatatc gtgctgaccc tgacactgtt 2221 tgaggacaga gagatgatcg aggaacggct gaaaacctat gcccacctgt tcgacgacaa 2281 agtgatgaag cagctgaagc ggcggagata caccggctgg ggcaggctga gccggaagct 2341 gatcaacggc atccgggaca agcagtccgg caagacaatc ctggatttcc tgaagtccga 2401 cggcttcgcc aacagaaact tcatgcagct gatccacgac gacagcctga cctttaaaga 2461 ggacatccag aaagcccagg tgtccggcca gggcgatagc ctgcacgagc acattgccaa 2521 tctggcaggc agccccgcca ttaagaaggg catcctgcaa acagtgaagg tggtggacga 2581 gctcgtgaaa gtgatgggac ggcacaagcc cgagaacatc gtgatcgaaa tggccagaga 2641 gaaccagacc acccagaagg gacagaagaa cagccgcgag agaatgaagc ggatcgaaga 2701 gggcatcaaa gagctgggca gccagatcct gaaagaacac cccgtggaaa acacccagct 2761 tcagaacgag aagctgtacc tgtactacct gcaaaatggg cgggatatgt acgtggacca 2821 ggaactggac atcaaccggc tgtccgacta cgatgtggac gctatcgtgc ctcagagctt 2881 tctgaaggac gactccatcg acaacaaggt gctgaccaga agcgacaaga accggggcaa 2941 gagcgacaac gtgccctccg aagaggtcgt gaagaagatg aagaactact ggcggcagct 3001 gctgaacgcc aagctgatta cccagagaaa gttcgacaat ctgaccaagg ccgagagagg 3061 cggcctgagc gaactggata aggccggatt catcaagaga cagctggtgg aaacccggca 3121 gatcacaaag cacgtggcac agatcctgga ctcccggatg aacactaagt acgacgagaa 3181 tgacaagctg atccgggaag tgaaagtgat caccctgaag tccaagctgg tgtccgattt 3241 ccggaaggat ttccagtttt acaaagtgcg cgagatcaac aactaccacc acgcccacga 3301 cgcctacctg aacgccgtcg tgggaaccgc cctgatcaaa aagtacccta agctggaaag 3361 cgagttcgtg tacggcgact acaaggtgta cgacgtgcgg aagatgatcg ccaagagcga 3421 gcaggaaatc ggcaaggcta ccgccaagta cttcttctac agcaacatca tgaacttttt 3481 caagaccgag attaccctgg ccaacggcga gatccggaag cggcctctga tcgagacaaa 3541 cggcgaaacc ggggagatcg tgtgggataa gggccgggat tttgccaccg tgcggaaagt 3601 gctgagcatg ccccaagtga atatcgtgaa aaagaccgag gtgcagacag gcggcttcag 3661 caaagagtct atcctgccca agaggaacag cgataagctg atcgccagaa agaaggactg 3721 ggaccctaag aagtacggcg gcttcgacag ccccaccgtg gcctattctg tgctggtggt 3781 ggccaaagtg gaaaagggca agtccaagaa actgaagagt gtgaaagagc tgctggggat 3841 caccatcatg gaaagaagca gcttcgagaa gaatcccatc gactttctgg aagccaaggg 3901 ctacaaagaa gtgaaaaagg acctgatcat caagctgcct aagtactccc tgttcgagct 3961 ggaaaacggc cggaagagaa tgctggcctc tgcgggcgaa ctgcaaaagg gaaacgaact 4021 ggccctgccc tccaaatatg tgaacttcct gtacctggcc agccactatg agaagctgaa 4081 gggctccccc gaggataatg agcagaaaca gctgtttgtg gaacagcaca agcactacct 4141 ggacgagatc atcgagcaga tcagcgagtt ctccaagaga gtgatcctgg ccgacgctaa 4201 tctggacaaa gtgctgtccg cctacaacaa gcaccgggat aagcccatca gagagcaggc 4261 cgagaatatc atccacctgt ttaccctgac caatctggga gcccctgccg ccttcaagta 4321 ctttgacacc accatcgacc ggaagaggta caccagcacc aaagaggtgc tggacgccac 4381 cctgatccac cagagcatca ccggcctgta cgagacacgg atcgacctgt ctcagctggg 4441 aggcgactac tagagcctgc aagaaaaaga ttgaattact agaggtgcct tctagttgcc 4501 agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca 4561 ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta 4621 ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc 4681 atgctgggga tgcggtgggc tctatg //