LOCUS BBa_K1150049 3881 bp DNA linear 14-Nov-2024 DEFINITION Truncated CMV dCas9 Device #3 ACCESSION BBa_K1150049 VERSION BBa_K1150049.1 FEATURES Location/Qualifiers promoter 1..580 /label=CMV promoter 1..580 /label=BBa_K747096 misc_feature 589..615 /label=HA misc_feature 589..615 /label=BBa_K1150016 CDS 624..644 /label=NLS misc_feature 624..644 /label=BBa_K1150010 CDS 653..3622 /label=BBa_K1150066 CDS 3631..3651 /label=NLS misc_feature 3631..3651 /label=BBa_K1150010 misc_feature 3660..3881 /label=BGH terminator 3660..3881 /label=BBa_K1150012 ORIGIN 1 atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata 61 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat 121 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga 181 gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc 241 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt 301 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat 361 gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag 421 tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc 481 aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga 541 ggtctatata agcagagctg gtttagtgaa ccgtcagatc tactagagta cccatacgat 601 gttccagatt acgcttacta gagcctgcaa gaaaaagatt gaattactag aggacaagaa 661 gtacagcatc ggcctggcca tcggcctcgt gaagctgaac agagaggacc tgctgcggaa 721 gcagcggacc ttcgacaacg gcagcatccc ccaccagatc cacctgggag agctgcacgc 781 cattctgcgg cggcaggaag atttttaccc attcctgaag gacaaccggg aaaagatcga 841 gaagatcctg accttccgca tcccctacta cgtgggccct ctggccaggg gaaacagcag 901 attcgcctgg atgaccagaa agagcgagga aaccatcacc ccctggaact tcgaggaagt 961 ggtggacaag ggcgcttccg cccagagctt catcgagcgg atgaccaact tcgataagaa 1021 cctgcccaac gagaaggtgc tgcccaagca cagcctgctg tacgagtact tcaccgtgta 1081 taacgagctg accaaagtga aatacgtgac cgagggaatg agaaagcccg ccttcctgag 1141 cggcgagcag aaaaaggcca tcgtggacct gctgttcaag accaaccgga aagtgaccgt 1201 gaagcagctg aaagaggact acttcaagaa aatcgagtgc ttcgactccg tggaaatctc 1261 cggcgtggaa gatcggttca acgcctccct gggcacatac cacgatctgc tgaaaattat 1321 caaggacaag gacttcctgg acaatgagga aaacgaggac attctggaag atatcgtgct 1381 gaccctgaca ctgtttgagg acagagagat gatcgaggaa cggctgaaaa cctatgccca 1441 cctgttcgac gacaaagtga tgaagcagct gaagcggcgg agatacaccg gctggggcag 1501 gctgagccgg aagctgatca acggcatccg ggacaagcag tccggcaaga caatcctgga 1561 tttcctgaag tccgacggct tcgccaacag aaacttcatg cagctgatcc acgacgacag 1621 cctgaccttt aaagaggaca tccagaaagc ccaggtgtcc ggccagggcg atagcctgca 1681 cgagcacatt gccaatctgg caggcagccc cgccattaag aagggcatcc tgcaaacagt 1741 gaaggtggtg gacgagctcg tgaaagtgat gggacggcac aagcccgaga acatcgtgat 1801 cgaaatggcc agagagaacc agaccaccca gaagggacag aagaacagcc gcgagagaat 1861 gaagcggatc gaagagggca tcaaagagct gggcagccag atcctgaaag aacaccccgt 1921 ggaaaacacc cagcttcaga acgagaagct gtacctgtac tacctgcaaa atgggcggga 1981 tatgtacgtg gaccaggaac tggacatcaa ccggctgtcc gactacgatg tggacgctat 2041 cgtgcctcag agctttctga aggacgactc catcgacaac aaggtgctga ccagaagcga 2101 caagaaccgg ggcaagagcg acaacgtgcc ctccgaagag gtcgtgaaga agatgaagaa 2161 ctactggcgg cagctgctga acgccaagct gattacccag agaaagttcg acaatctgac 2221 caaggccgag agaggcggcc tgagcgaact ggataaggcc ggattcatca agagacagct 2281 ggtggaaacc cggcagatca caaagcacgt ggcacagatc ctggactccc ggatgaacac 2341 taagtacgac gagaatgaca agctgatccg ggaagtgaaa gtgatcaccc tgaagtccaa 2401 gctggtgtcc gatttccgga aggatttcca gttttacaaa gtgcgcgaga tcaacaacta 2461 ccaccacgcc cacgacgcct acctgaacgc cgtcgtggga accgccctga tcaaaaagta 2521 ccctaagctg gaaagcgagt tcgtgtacgg cgactacaag gtgtacgacg tgcggaagat 2581 gatcgccaag agcgagcagg aaatcggcaa ggctaccgcc aagtacttct tctacagcaa 2641 catcatgaac tttttcaaga ccgagattac cctggccaac ggcgagatcc ggaagcggcc 2701 tctgatcgag acaaacggcg aaaccgggga gatcgtgtgg gataagggcc gggattttgc 2761 caccgtgcgg aaagtgctga gcatgcccca agtgaatatc gtgaaaaaga ccgaggtgca 2821 gacaggcggc ttcagcaaag agtctatcct gcccaagagg aacagcgata agctgatcgc 2881 cagaaagaag gactgggacc ctaagaagta cggcggcttc gacagcccca ccgtggccta 2941 ttctgtgctg gtggtggcca aagtggaaaa gggcaagtcc aagaaactga agagtgtgaa 3001 agagctgctg gggatcacca tcatggaaag aagcagcttc gagaagaatc ccatcgactt 3061 tctggaagcc aagggctaca aagaagtgaa aaaggacctg atcatcaagc tgcctaagta 3121 ctccctgttc gagctggaaa acggccggaa gagaatgctg gcctctgcgg gcgaactgca 3181 aaagggaaac gaactggccc tgccctccaa atatgtgaac ttcctgtacc tggccagcca 3241 ctatgagaag ctgaagggct cccccgagga taatgagcag aaacagctgt ttgtggaaca 3301 gcacaagcac tacctggacg agatcatcga gcagatcagc gagttctcca agagagtgat 3361 cctggccgac gctaatctgg acaaagtgct gtccgcctac aacaagcacc gggataagcc 3421 catcagagag caggccgaga atatcatcca cctgtttacc ctgaccaatc tgggagcccc 3481 tgccgccttc aagtactttg acaccaccat cgaccggaag aggtacacca gcaccaaaga 3541 ggtgctggac gccaccctga tccaccagag catcaccggc ctgtacgaga cacggatcga 3601 cctgtctcag ctgggaggcg actactagag cctgcaagaa aaagattgaa ttactagagg 3661 tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg 3721 aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga 3781 gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg 3841 aagacaatag caggcatgct ggggatgcgg tgggctctat g //