LOCUS BBa_K1150050 3626 bp DNA linear 22-Nov-2024 DEFINITION Truncated CMV dCas9 Device #4 ACCESSION BBa_K1150050 VERSION BBa_K1150050.1 FEATURES Location/Qualifiers promoter 1..580 /label=CMV promoter 1..580 /label=BBa_K747096 misc_feature 589..615 /label=HA misc_feature 589..615 /label=BBa_K1150016 CDS 624..644 /label=NLS misc_feature 624..644 /label=BBa_K1150010 CDS 653..3367 /label=BBa_K1150067 CDS 3376..3396 /label=NLS misc_feature 3376..3396 /label=BBa_K1150010 misc_feature 3405..3626 /label=BGH terminator 3405..3626 /label=BBa_K1150012 ORIGIN 1 atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata 61 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat 121 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga 181 gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc 241 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt 301 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat 361 gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag 421 tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc 481 aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga 541 ggtctatata agcagagctg gtttagtgaa ccgtcagatc tactagagta cccatacgat 601 gttccagatt acgcttacta gagcctgcaa gaaaaagatt gaattactag aggacaagaa 661 gtacagcatc ggcctggcca tcggcaccaa ctctgtgggc tgggccgtga tcaccgacga 721 gtacaaggtg cccagcaaga aattcaaggt gctgggcaac accgaccggc acagcatcaa 781 gaagaacctg atcggagccc tgctgttcga cagcggcgaa acagccgagg ccacccggct 841 gaagagaacc gccagaagaa gatacaccag acggaagaac cggatctgct atctgcaaga 901 gatcttcagc aacgagatgg ccaaggtgga cgacagcttc ttccacagac tggaagagtc 961 cttcctggtg gaagaggata agaagcacga gcggcacccc atcttcggca acatcgtgga 1021 cgaggtggcc taccacgaga agtaccccac catctaccac ctgagaaaga aactggtgga 1081 cagcaccgac aaggccgacc tgcggctgat ctatctggcc ctggcccaca tgatcaagtt 1141 ccggggccac ttcctgatcg agggcgacct gaaccccgac aacagcgacg tggacaagct 1201 gttcatccag ctggtgcaga cctacaacca gctgttcgag gaaaacccca tcaacgccag 1261 cggcgtggac gccaaggcca tcctgtctgc cagactgagc aagagcagac ggctggaaaa 1321 tctgatcgcc cagctgcccg gcgagaagaa gaatggcctg ttcggcaacc tgattgccct 1381 gagcctgggc ctgaccccca acttcaagag caacttcgac ctggccgagg atgccaaact 1441 tcagctgagc aaggacacct acgacgacga cctggacaac ctgctggccc agatcggcga 1501 ccagtacgcc gacctgtttc tggccgccaa gaacctgtcc gacgccatcc tgctgagcga 1561 catcctgaga gtgaacaccg agatcaccaa ggcccccctg agcgcctcta tgatcaagag 1621 atacgacgag caccaccagg acctgaccct gctgaaagct ctcgtgcggc agcagctgcc 1681 tgagaagtac aaagagattt tcttcgacca gagcaagaac ggctacgccg gatacattga 1741 cggcggagcc agccaggaag agttctacaa gttcatcaag cccatcctgg aaaagatgga 1801 cggcaccgag gaactgctcg tgaagctgaa cagagaggac ctgctgcgga agcagcggac 1861 cttcgacaac ggcagcatcc cccaccagat ccacctggga gagctgcacg ccattctgcg 1921 gcggcaggcc aagctgatta cccagagaaa gttcgacaat ctgaccaagg ccgagagagg 1981 cggcctgagc gaactggata aggccggatt catcaagaga cagctggtgg aaacccggca 2041 gatcacaaag cacgtggcac agatcctgga ctcccggatg aacactaagt acgacgagaa 2101 tgacaagctg atccgggaag tgaaagtgat caccctgaag tccaagctgg tgtccgattt 2161 ccggaaggat ttccagtttt acaaagtgcg cgagatcaac aactaccacc acgcccacga 2221 cgcctacctg aacgccgtcg tgggaaccgc cctgatcaaa aagtacccta agctggaaag 2281 cgagttcgtg tacggcgact acaaggtgta cgacgtgcgg aagatgatcg ccaagagcga 2341 gcaggaaatc ggcaaggcta ccgccaagta cttcttctac agcaacatca tgaacttttt 2401 caagaccgag attaccctgg ccaacggcga gatccggaag cggcctctga tcgagacaaa 2461 cggcgaaacc ggggagatcg tgtgggataa gggccgggat tttgccaccg tgcggaaagt 2521 gctgagcatg ccccaagtga atatcgtgaa aaagaccgag gtgcagacag gcggcttcag 2581 caaagagtct atcctgccca agaggaacag cgataagctg atcgccagaa agaaggactg 2641 ggaccctaag aagtacggcg gcttcgacag ccccaccgtg gcctattctg tgctggtggt 2701 ggccaaagtg gaaaagggca agtccaagaa actgaagagt gtgaaagagc tgctggggat 2761 caccatcatg gaaagaagca gcttcgagaa gaatcccatc gactttctgg aagccaaggg 2821 ctacaaagaa gtgaaaaagg acctgatcat caagctgcct aagtactccc tgttcgagct 2881 ggaaaacggc cggaagagaa tgctggcctc tgcgggcgaa ctgcaaaagg gaaacgaact 2941 ggccctgccc tccaaatatg tgaacttcct gtacctggcc agccactatg agaagctgaa 3001 gggctccccc gaggataatg agcagaaaca gctgtttgtg gaacagcaca agcactacct 3061 ggacgagatc atcgagcaga tcagcgagtt ctccaagaga gtgatcctgg ccgacgctaa 3121 tctggacaaa gtgctgtccg cctacaacaa gcaccgggat aagcccatca gagagcaggc 3181 cgagaatatc atccacctgt ttaccctgac caatctggga gcccctgccg ccttcaagta 3241 ctttgacacc accatcgacc ggaagaggta caccagcacc aaagaggtgc tggacgccac 3301 cctgatccac cagagcatca ccggcctgta cgagacacgg atcgacctgt ctcagctggg 3361 aggcgactac tagagcctgc aagaaaaaga ttgaattact agaggtgcct tctagttgcc 3421 agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca 3481 ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta 3541 ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc 3601 atgctgggga tgcggtgggc tctatg //