LOCUS BBa_K1150051 3722 bp DNA linear 14-Nov-2024 DEFINITION Truncated CMV dCas9 Device #5 ACCESSION BBa_K1150051 VERSION BBa_K1150051.1 FEATURES Location/Qualifiers promoter 1..580 /label=CMV promoter 1..580 /label=BBa_K747096 misc_feature 589..615 /label=HA misc_feature 589..615 /label=BBa_K1150016 CDS 624..644 /label=NLS misc_feature 624..644 /label=BBa_K1150010 CDS 653..3463 /label=BBa_K1150068 CDS 3472..3492 /label=NLS misc_feature 3472..3492 /label=BBa_K1150010 misc_feature 3501..3722 /label=BGH terminator 3501..3722 /label=BBa_K1150012 ORIGIN 1 atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata 61 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat 121 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga 181 gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc 241 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt 301 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat 361 gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag 421 tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc 481 aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga 541 ggtctatata agcagagctg gtttagtgaa ccgtcagatc tactagagta cccatacgat 601 gttccagatt acgcttacta gagcctgcaa gaaaaagatt gaattactag aggacaagaa 661 gtacagcatc ggcctggcca tcggcaccaa ctctgtgggc tgggccgtga tcaccgacga 721 gtacaaggtg cccagcaaga aattcaaggt gctgggcaac accgaccggc acagcatcaa 781 gaagaacctg atcggagccc tgctgttcga cagcggcgaa acagccgagg ccacccggct 841 gaagagaacc gccagaagaa gatacaccag acggaagaac cggatctgct atctgcaaga 901 gatcttcagc aacgagatgg ccaaggtgga cgacagcttc ttccacagac tggaagagtc 961 cttcctggtg gaagaggata agaagcacga gcggcacccc atcttcggca acatcgtgga 1021 cgaggtggcc taccacgaga agtaccccac catctaccac ctgagaaaga aactggtgga 1081 cagcaccgac aaggccgacc tgcggctgat ctatctggcc ctggcccaca tgatcaagtt 1141 ccggggccac ttcctgatcg agggcgacct gaaccccgac aacagcgacg tggacaagct 1201 gttcatccag ctggtgcaga cctacaacca gctgttcgag gaaaacccca tcaacgccag 1261 cggcgtggac gccaaggcca tcctgtctgc cagactgagc aagagcagac ggctggaaaa 1321 tctgatcgcc cagctgcccg gcgagaagaa gaatggcctg ttcggcaacc tgattgccct 1381 gagcctgggc ctgaccccca acttcaagag caacttcgac ctggccgagg atgccaaact 1441 tcagctgagc aaggacacct acgacgacga cctggacaac ctgctggccc agatcggcga 1501 ccagtacgcc gacctgtttc tggccgccaa gaacctgtcc gacgccatcc tgctgagcga 1561 catcctgaga gtgaacaccg agatcaccaa ggcccccctg agcgcctcta tgatcaagag 1621 atacgacgag caccaccagg acctgaccct gctgaaagct ctcgtgcggc agcagctgcc 1681 tgagaagtac aaagagattt tcttcgacca gagcaagaac ggctacgccg gatacattga 1741 cggcggagcc agccaggaag agttctacaa gttcatcaag cccatcctgg aaaagatgga 1801 cggcaccgag gaactgctcg tgaagctgaa cagagaggac ctgctgcgga agcagcggac 1861 cttcgacaac ggcagcatcc cccaccagat ccacctggga gagctgcacg ccattctgcg 1921 gcggcaggaa gatttttacc cattcctgaa ggacaaccgg gaaaagatcg agaagatcct 1981 gaccttccgc atcccctact acgtgggccc tctggccagg ggaaacagca gattcgcctg 2041 gatgaccaga aagagcgagg aaaccatcac cccctggaac ttcgaggaag tggtggacaa 2101 gggcgcttcc gcccagagct tcatcgagcg gatgaccaac ttcgataaga acctgcccaa 2161 cgagaaggtg ctgcccaagc acagcctgct gtacgagtac ttcaccgtgt ataacgagct 2221 gaccaaagtg aaatacgtga ccgagggaat gagaaagccc gccttcctga gcggcgagca 2281 gaaaaaggcc atcgtggacc tgctgttcaa gaccaaccgg aaagtgaccg tgaagcagct 2341 gaaagaggac tacttcaaga aaatcgagtg cttcgactcc gtggaaatct ccggcgtgga 2401 agatcggttc aacgcctccc tgggcacata ccacgatctg ctgaaaatta tcaaggacaa 2461 ggacttcctg gacaatgagg aaaacgagga cattctggaa gatatcgtgc tgaccctgac 2521 actgtttgag gacagagaga tgatcgagga acggctgaaa acctatgccc acctgttcga 2581 cgacaaagtg atgaagcagc tgaagcggcg gagatacacc ggctggggca ggctgagccg 2641 gaagctgatc aacggcatcc gggacaagca gtccggcaag acaatcctgg atttcctgaa 2701 gtccgacggc ttcgccaaca gaaacttcat gcagctgatc cacgacgaca gcctgacctt 2761 taaagaggac atccagaaag cccaggtgtc cggccagggc gatagcctgc acgagcacat 2821 tgccaatctg gcaggcagcc ccgccattaa gaagggcatc ctgcaaacag tgaaggtggt 2881 ggacgagctc gtgaaagtga tgggacggca caagcccgag aacatcgtga tcgaaatggc 2941 cagagagaac cagaccaccc agaagggaca gaagaacagc cgcgagagaa tgaagcggat 3001 cgaagagggc atcaaagagc tgggcagcca gatcctgaaa gaacaccccg tggaaaacac 3061 ccagcttcag aacgagaagc tgtacctgta ctacctgcaa aatgggcggg atatgtacgt 3121 ggaccaggaa ctggacatca accggctgtc cgactacgat gtggacgcta tcgtgcctca 3181 gagctttctg aaggacgact ccatcgacaa caaggtgctg accagaagcg acaagaaccg 3241 gggcaagagc gacaacgtgc cctccgaaga ggtcgtgaag aagatgaaga actactggcg 3301 gcagctgctg aacgccaagc tgattaccca gagaaagttc gacaatctga ccaaggccga 3361 gagaggcggc ctgagcgaac tggtgctgga cgccaccctg atccaccaga gcatcaccgg 3421 cctgtacgag acacggatcg acctgtctca gctgggaggc gactactaga gcctgcaaga 3481 aaaagattga attactagag gtgccttcta gttgccagcc atctgttgtt tgcccctccc 3541 ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg 3601 aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg 3661 acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta 3721 tg //