LOCUS BBa_K1150052 4440 bp DNA linear 14-Nov-2024 DEFINITION Truncated SV40 dCas9 Device #1 ACCESSION BBa_K1150052 VERSION BBa_K1150052.1 FEATURES Location/Qualifiers promoter 1..344 /label=SV40 promoter 1..344 /label=BBa_K1150011 misc_feature 353..379 /label=HA misc_feature 353..379 /label=BBa_K1150016 CDS 388..408 /label=NLS misc_feature 388..408 /label=BBa_K1150010 misc_feature 2931..2933 /label=Nickaseless mutation CDS 417..4181 /label=BBa_K1150064 CDS 4190..4210 /label=NLS misc_feature 4190..4210 /label=BBa_K1150010 misc_feature 4219..4440 /label=BGH terminator 4219..4440 /label=BBa_K1150012 ORIGIN 1 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt 61 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca 121 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta 181 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga 241 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag 301 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agcttactag agtacccata 361 cgatgttcca gattacgctt actagagcct gcaagaaaaa gattgaatta ctagaggaca 421 agaagtacag catcggcctg gccatcggca ccaactctgt gggctgggcc gtgatcaccg 481 acgagtacaa ggtgcccagc aagaaattca aggtgctggg caacaccgac cggcacagca 541 tcaagaagaa cctgatcgga gccctgctgt tcgacagcgg cgaaacagcc gaggccaccc 601 ggctgaagag aaccgccaga agaagataca ccagacggaa gaaccggatc tgctatctgc 661 aagagatctt cagcaacgag atggccaagg tggacgacag cttcttccac agactggaag 721 agtccttcct ggtggaagag gataagaagc acgagcggca ccccatcttc ggcaacatcg 781 tggacgaggt ggcctaccac gagaagtacc ccaccatcta ccacctgaga aagaaactgg 841 tggacagcac cgacaaggcc gacctgcggc tgatctatct ggccctggcc cacatgatca 901 agttccgggg ccacttcctg atcgagggcg acctgaaccc cgacaacagc gacgtggaca 961 agctgttcat ccagctggtg cagacctaca accagctgtt cgaggaaaac cccatcaacg 1021 ccagcggcgt ggacgccaag gccatcctgt ctgccagact gagcaagagc agacggctgg 1081 aaaatctgat cgcccagctg cccggcgaga agaagaatgg cctgttcggc aacctgattg 1141 ccctgagcct gggcctgacc cccaacttca agagcaactt cgacctggcc gaggatgcca 1201 aacttcagct gagcaaggac acctacgacg acgacctgga caacctgctg gcccagatcg 1261 gcgaccagta cgccgacctg tttctggccg ccaagaacct gtccgacgcc atcctgctga 1321 gcgacatcct gagagtgaac accgagatca ccaaggcccc cctgagcgcc tctatgatca 1381 agagatacga cgagcaccac caggacctga ccctgctgaa agctctcgtg cggcagcagc 1441 tgcctgagaa gtacaaagag attttcttcg accagagcaa gaacggctac gccggataca 1501 ttgacggcgg agccagccag gaagagttct acaagttcat caagcccatc ctggaaaaga 1561 tggacggcac cgaggaactg ctcgtgaagc tgaacagaga ggacctgctg cggaagcagc 1621 ggaccttcga caacggcagc atcccccacc agatccacct gggagagctg cacgccattc 1681 tgcggcggca ggaagatttt tacccattcc tgaaggacaa ccgggaaaag atcgagaaga 1741 tcctgacctt ccgcatcccc tactacgtgg gccctctggc caggggaaac agcagattcg 1801 cctggatgac cagaaagagc gaggaaacca tcaccccctg gaacttcgag gaagtggtgg 1861 acaagggcgc ttccgcccag agcttcatcg agcggatgac caacttcgat aagaacctgc 1921 ccaacgagaa ggtgctgccc aagcacagcc tgctgtacga gtacttcacc gtgtataacg 1981 agctgaccaa agtgaaatac gtgaccgagg gaatgagaaa gcccgccttc ctgagcggcg 2041 agcagaaaaa ggccatcgtg gacctgctgt tcaagaccaa ccggaaagtg accgtgaagc 2101 agctgaaaga ggactacttc aagaaaatcg agtgcttcga ctccgtggaa atctccggcg 2161 tggaagatcg gttcaacgcc tccctgggca cataccacga tctgctgaaa attatcaagg 2221 acaaggactt cctggacaat gaggaaaacg aggacattct ggaagatatc gtgctgaccc 2281 tgacactgtt tgaggacaga gagatgatcg aggaacggct gaaaacctat gcccacctgt 2341 tcgacgacaa agtgatgaag cagctgaagc ggcggagata caccggctgg ggcaggctga 2401 gccggaagct gatcaacggc atccgggaca agcagtccgg caagacaatc ctggatttcc 2461 tgaagtccga cggcttcgcc aacagaaact tcatgcagct gatccacgac gacagcctga 2521 cctttaaaga ggacatccag aaagcccagg tgtccggcca gggcgatagc ctgcacgagc 2581 acattgccaa tctggcaggc agccccgcca ttaagaaggg catcctgcaa acagtgaagg 2641 tggtggacga gctcgtgaaa gtgatgggac ggcacaagcc cgagaacatc gtgatcgaaa 2701 tggccagaga gaaccagacc acccagaagg gacagaagaa cagccgcgag agaatgaagc 2761 ggatcgaaga gggcatcaaa gagctgggca gccagatcct gaaagaacac cccgtggaaa 2821 acacccagct tcagaacgag aagctgtacc tgtactacct gcaaaatggg cgggatatgt 2881 acgtggacca ggaactggac atcaaccggc tgtccgacta cgatgtggac gctatcgtgc 2941 ctcagagctt tctgaaggac gactccatcg acaacaaggt gctgaccaga agcgacaaga 3001 accggggcaa gagcgacaac gtgccctccg aagaggtcgt gaagaagatg aagaactact 3061 ggcggcagct gctgaacgcc aagctgatta cccagagaaa gttcgacaat ctgaccaagg 3121 ccgagagagg cggcctgagc gaactggata aggccggatt catcaagaga cagctggtgg 3181 aaacccggca gatcacaaag cacgtggcac agatcctgga ctcccggatg aacactaagt 3241 acgacgagaa tgacaagctg atccgggaag tgaaagtgat caccctgaag tccaagctgg 3301 tgtccgattt ccggaaggat ttccagtttt acaaagtgcg cgagatcaac aactaccacc 3361 acgcccacga cgcctacctg aacgccgtcg tgggaaccgc cctgatcaaa aagtacccta 3421 agctggaaag cgagttcgtg tacggcgact acaaggtgta cgacgtgcgg aagatgatcg 3481 ccaagagcga gcaggaaatc ggcaaggcta ccgccaagta cttcttctac agcaacatca 3541 tgaacttttt caagaccgag attaccctgg ccaacggcga gatccggaag cggcctctga 3601 tcgagacaaa cggcgaaacc ggggagatcg tgtgggataa gggccgggat tttgccaccg 3661 tgcggaaagt gctgagcatg ccccaagtga atatcgtgaa aaagaccgag gtgcagacag 3721 gcggcttcag caaagagtct atcctgccca agaggaacag cgataagctg atcgccagaa 3781 agaaggactg ggaccctaag aagtacggcg gcttcgacag ccccaccgtg gcctattctg 3841 tgctggtggt ggccaaagtg gaaaagggca agtccaagaa actgaagagt gtgaaagagc 3901 tgctggggat caccatcatg gaaagaagca gcttcgagaa gaatcccatc gactttctgg 3961 aagccaaggg ctacaaagaa gtgaaaaagg acctgatcat caagctgcct aagtactccc 4021 tgttcgagct ggaaaacggc cggaagagaa tgctggcctc tgcgggcgaa ctgcaaaagg 4081 gaaacgaact ggccctgccc gtgctggacg ccaccctgat ccaccagagc atcaccggcc 4141 tgtacgagac acggatcgac ctgtctcagc tgggaggcga ctactagagc ctgcaagaaa 4201 aagattgaat tactagaggt gccttctagt tgccagccat ctgttgtttg cccctccccc 4261 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa 4321 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac 4381 agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg //