LOCUS       BBa_K1150055            3390 bp    DNA     linear       14-Nov-2024
DEFINITION  Truncated SV40 dCas9 Device #4
ACCESSION   BBa_K1150055
VERSION     BBa_K1150055.1  
FEATURES             Location/Qualifiers
     promoter        1..344
                     /label=SV40
     promoter        1..344
                     /label=BBa_K1150011
     misc_feature    353..379
                     /label=HA
     misc_feature    353..379
                     /label=BBa_K1150016
     CDS             388..408
                     /label=NLS
     misc_feature    388..408
                     /label=BBa_K1150010
     CDS             417..3131
                     /label=BBa_K1150067
     CDS             3140..3160
                     /label=NLS
     misc_feature    3140..3160
                     /label=BBa_K1150010
     misc_feature    3169..3390
                     /label=BGH
     terminator      3169..3390
                     /label=BBa_K1150012
ORIGIN
        1 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
       61 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
      121 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
      181 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
      241 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
      301 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agcttactag agtacccata
      361 cgatgttcca gattacgctt actagagcct gcaagaaaaa gattgaatta ctagaggaca
      421 agaagtacag catcggcctg gccatcggca ccaactctgt gggctgggcc gtgatcaccg
      481 acgagtacaa ggtgcccagc aagaaattca aggtgctggg caacaccgac cggcacagca
      541 tcaagaagaa cctgatcgga gccctgctgt tcgacagcgg cgaaacagcc gaggccaccc
      601 ggctgaagag aaccgccaga agaagataca ccagacggaa gaaccggatc tgctatctgc
      661 aagagatctt cagcaacgag atggccaagg tggacgacag cttcttccac agactggaag
      721 agtccttcct ggtggaagag gataagaagc acgagcggca ccccatcttc ggcaacatcg
      781 tggacgaggt ggcctaccac gagaagtacc ccaccatcta ccacctgaga aagaaactgg
      841 tggacagcac cgacaaggcc gacctgcggc tgatctatct ggccctggcc cacatgatca
      901 agttccgggg ccacttcctg atcgagggcg acctgaaccc cgacaacagc gacgtggaca
      961 agctgttcat ccagctggtg cagacctaca accagctgtt cgaggaaaac cccatcaacg
     1021 ccagcggcgt ggacgccaag gccatcctgt ctgccagact gagcaagagc agacggctgg
     1081 aaaatctgat cgcccagctg cccggcgaga agaagaatgg cctgttcggc aacctgattg
     1141 ccctgagcct gggcctgacc cccaacttca agagcaactt cgacctggcc gaggatgcca
     1201 aacttcagct gagcaaggac acctacgacg acgacctgga caacctgctg gcccagatcg
     1261 gcgaccagta cgccgacctg tttctggccg ccaagaacct gtccgacgcc atcctgctga
     1321 gcgacatcct gagagtgaac accgagatca ccaaggcccc cctgagcgcc tctatgatca
     1381 agagatacga cgagcaccac caggacctga ccctgctgaa agctctcgtg cggcagcagc
     1441 tgcctgagaa gtacaaagag attttcttcg accagagcaa gaacggctac gccggataca
     1501 ttgacggcgg agccagccag gaagagttct acaagttcat caagcccatc ctggaaaaga
     1561 tggacggcac cgaggaactg ctcgtgaagc tgaacagaga ggacctgctg cggaagcagc
     1621 ggaccttcga caacggcagc atcccccacc agatccacct gggagagctg cacgccattc
     1681 tgcggcggca ggccaagctg attacccaga gaaagttcga caatctgacc aaggccgaga
     1741 gaggcggcct gagcgaactg gataaggccg gattcatcaa gagacagctg gtggaaaccc
     1801 ggcagatcac aaagcacgtg gcacagatcc tggactcccg gatgaacact aagtacgacg
     1861 agaatgacaa gctgatccgg gaagtgaaag tgatcaccct gaagtccaag ctggtgtccg
     1921 atttccggaa ggatttccag ttttacaaag tgcgcgagat caacaactac caccacgccc
     1981 acgacgccta cctgaacgcc gtcgtgggaa ccgccctgat caaaaagtac cctaagctgg
     2041 aaagcgagtt cgtgtacggc gactacaagg tgtacgacgt gcggaagatg atcgccaaga
     2101 gcgagcagga aatcggcaag gctaccgcca agtacttctt ctacagcaac atcatgaact
     2161 ttttcaagac cgagattacc ctggccaacg gcgagatccg gaagcggcct ctgatcgaga
     2221 caaacggcga aaccggggag atcgtgtggg ataagggccg ggattttgcc accgtgcgga
     2281 aagtgctgag catgccccaa gtgaatatcg tgaaaaagac cgaggtgcag acaggcggct
     2341 tcagcaaaga gtctatcctg cccaagagga acagcgataa gctgatcgcc agaaagaagg
     2401 actgggaccc taagaagtac ggcggcttcg acagccccac cgtggcctat tctgtgctgg
     2461 tggtggccaa agtggaaaag ggcaagtcca agaaactgaa gagtgtgaaa gagctgctgg
     2521 ggatcaccat catggaaaga agcagcttcg agaagaatcc catcgacttt ctggaagcca
     2581 agggctacaa agaagtgaaa aaggacctga tcatcaagct gcctaagtac tccctgttcg
     2641 agctggaaaa cggccggaag agaatgctgg cctctgcggg cgaactgcaa aagggaaacg
     2701 aactggccct gccctccaaa tatgtgaact tcctgtacct ggccagccac tatgagaagc
     2761 tgaagggctc ccccgaggat aatgagcaga aacagctgtt tgtggaacag cacaagcact
     2821 acctggacga gatcatcgag cagatcagcg agttctccaa gagagtgatc ctggccgacg
     2881 ctaatctgga caaagtgctg tccgcctaca acaagcaccg ggataagccc atcagagagc
     2941 aggccgagaa tatcatccac ctgtttaccc tgaccaatct gggagcccct gccgccttca
     3001 agtactttga caccaccatc gaccggaaga ggtacaccag caccaaagag gtgctggacg
     3061 ccaccctgat ccaccagagc atcaccggcc tgtacgagac acggatcgac ctgtctcagc
     3121 tgggaggcga ctactagagc ctgcaagaaa aagattgaat tactagaggt gccttctagt
     3181 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact
     3241 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat
     3301 tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc
     3361 aggcatgctg gggatgcggt gggctctatg
//