LOCUS       BBa_K1150056            3486 bp    DNA     linear       14-Nov-2024
DEFINITION  Truncated SV40 dCas9 Device #5
ACCESSION   BBa_K1150056
VERSION     BBa_K1150056.1  
FEATURES             Location/Qualifiers
     promoter        1..344
                     /label=SV40
     promoter        1..344
                     /label=BBa_K1150011
     misc_feature    353..379
                     /label=HA
     misc_feature    353..379
                     /label=BBa_K1150016
     CDS             388..408
                     /label=NLS
     misc_feature    388..408
                     /label=BBa_K1150010
     CDS             417..3227
                     /label=BBa_K1150068
     CDS             3236..3256
                     /label=NLS
     misc_feature    3236..3256
                     /label=BBa_K1150010
     misc_feature    3265..3486
                     /label=BGH
     terminator      3265..3486
                     /label=BBa_K1150012
ORIGIN
        1 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
       61 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
      121 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
      181 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
      241 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
      301 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agcttactag agtacccata
      361 cgatgttcca gattacgctt actagagcct gcaagaaaaa gattgaatta ctagaggaca
      421 agaagtacag catcggcctg gccatcggca ccaactctgt gggctgggcc gtgatcaccg
      481 acgagtacaa ggtgcccagc aagaaattca aggtgctggg caacaccgac cggcacagca
      541 tcaagaagaa cctgatcgga gccctgctgt tcgacagcgg cgaaacagcc gaggccaccc
      601 ggctgaagag aaccgccaga agaagataca ccagacggaa gaaccggatc tgctatctgc
      661 aagagatctt cagcaacgag atggccaagg tggacgacag cttcttccac agactggaag
      721 agtccttcct ggtggaagag gataagaagc acgagcggca ccccatcttc ggcaacatcg
      781 tggacgaggt ggcctaccac gagaagtacc ccaccatcta ccacctgaga aagaaactgg
      841 tggacagcac cgacaaggcc gacctgcggc tgatctatct ggccctggcc cacatgatca
      901 agttccgggg ccacttcctg atcgagggcg acctgaaccc cgacaacagc gacgtggaca
      961 agctgttcat ccagctggtg cagacctaca accagctgtt cgaggaaaac cccatcaacg
     1021 ccagcggcgt ggacgccaag gccatcctgt ctgccagact gagcaagagc agacggctgg
     1081 aaaatctgat cgcccagctg cccggcgaga agaagaatgg cctgttcggc aacctgattg
     1141 ccctgagcct gggcctgacc cccaacttca agagcaactt cgacctggcc gaggatgcca
     1201 aacttcagct gagcaaggac acctacgacg acgacctgga caacctgctg gcccagatcg
     1261 gcgaccagta cgccgacctg tttctggccg ccaagaacct gtccgacgcc atcctgctga
     1321 gcgacatcct gagagtgaac accgagatca ccaaggcccc cctgagcgcc tctatgatca
     1381 agagatacga cgagcaccac caggacctga ccctgctgaa agctctcgtg cggcagcagc
     1441 tgcctgagaa gtacaaagag attttcttcg accagagcaa gaacggctac gccggataca
     1501 ttgacggcgg agccagccag gaagagttct acaagttcat caagcccatc ctggaaaaga
     1561 tggacggcac cgaggaactg ctcgtgaagc tgaacagaga ggacctgctg cggaagcagc
     1621 ggaccttcga caacggcagc atcccccacc agatccacct gggagagctg cacgccattc
     1681 tgcggcggca ggaagatttt tacccattcc tgaaggacaa ccgggaaaag atcgagaaga
     1741 tcctgacctt ccgcatcccc tactacgtgg gccctctggc caggggaaac agcagattcg
     1801 cctggatgac cagaaagagc gaggaaacca tcaccccctg gaacttcgag gaagtggtgg
     1861 acaagggcgc ttccgcccag agcttcatcg agcggatgac caacttcgat aagaacctgc
     1921 ccaacgagaa ggtgctgccc aagcacagcc tgctgtacga gtacttcacc gtgtataacg
     1981 agctgaccaa agtgaaatac gtgaccgagg gaatgagaaa gcccgccttc ctgagcggcg
     2041 agcagaaaaa ggccatcgtg gacctgctgt tcaagaccaa ccggaaagtg accgtgaagc
     2101 agctgaaaga ggactacttc aagaaaatcg agtgcttcga ctccgtggaa atctccggcg
     2161 tggaagatcg gttcaacgcc tccctgggca cataccacga tctgctgaaa attatcaagg
     2221 acaaggactt cctggacaat gaggaaaacg aggacattct ggaagatatc gtgctgaccc
     2281 tgacactgtt tgaggacaga gagatgatcg aggaacggct gaaaacctat gcccacctgt
     2341 tcgacgacaa agtgatgaag cagctgaagc ggcggagata caccggctgg ggcaggctga
     2401 gccggaagct gatcaacggc atccgggaca agcagtccgg caagacaatc ctggatttcc
     2461 tgaagtccga cggcttcgcc aacagaaact tcatgcagct gatccacgac gacagcctga
     2521 cctttaaaga ggacatccag aaagcccagg tgtccggcca gggcgatagc ctgcacgagc
     2581 acattgccaa tctggcaggc agccccgcca ttaagaaggg catcctgcaa acagtgaagg
     2641 tggtggacga gctcgtgaaa gtgatgggac ggcacaagcc cgagaacatc gtgatcgaaa
     2701 tggccagaga gaaccagacc acccagaagg gacagaagaa cagccgcgag agaatgaagc
     2761 ggatcgaaga gggcatcaaa gagctgggca gccagatcct gaaagaacac cccgtggaaa
     2821 acacccagct tcagaacgag aagctgtacc tgtactacct gcaaaatggg cgggatatgt
     2881 acgtggacca ggaactggac atcaaccggc tgtccgacta cgatgtggac gctatcgtgc
     2941 ctcagagctt tctgaaggac gactccatcg acaacaaggt gctgaccaga agcgacaaga
     3001 accggggcaa gagcgacaac gtgccctccg aagaggtcgt gaagaagatg aagaactact
     3061 ggcggcagct gctgaacgcc aagctgatta cccagagaaa gttcgacaat ctgaccaagg
     3121 ccgagagagg cggcctgagc gaactggtgc tggacgccac cctgatccac cagagcatca
     3181 ccggcctgta cgagacacgg atcgacctgt ctcagctggg aggcgactac tagagcctgc
     3241 aagaaaaaga ttgaattact agaggtgcct tctagttgcc agccatctgt tgtttgcccc
     3301 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
     3361 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg
     3421 caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc
     3481 tctatg
//