##gff-version 3
##sequence-region BBa_K1150057 1 4837
BBa_K1150057	.	CDS	417	4181	.	+	0	ID=BBa_K1150064;Name=BBa_K1150064
BBa_K1150057	.	sequence_alteration	2931	2933	.	+	0	ID=annotation2354616;Name=Nickaseless mutation;Parent=BBa_K1150064
BBa_K1150057	.	terminator	4616	4837	.	+	0	ID=BBa_K1150012;Name=BBa_K1150012
BBa_K1150057	.	sequence_feature	4616	4837	.	+	0	ID=annotation2347917;Name=BGH;Parent=BBa_K1150012
BBa_K1150057	.	engineered_region	388	408	.	+	0	ID=BBa_K1150010;Name=BBa_K1150010
BBa_K1150057	.	CDS	388	408	.	+	0	ID=annotation2347465;Name=NLS;Parent=BBa_K1150010
BBa_K1150057	.	CDS	4207	4578	.	+	0	ID=BBa_K1150001;Name=BBa_K1150001
BBa_K1150057	.	sequence_feature	4207	4578	.	+	0	ID=annotation2347617;Name=VP16;Parent=BBa_K1150001
BBa_K1150057	.	promoter	1	344	.	+	0	ID=BBa_K1150011;Name=BBa_K1150011
BBa_K1150057	.	promoter	1	344	.	+	0	ID=annotation2347620;Name=SV40;Parent=BBa_K1150011
BBa_K1150057	.	tag	353	379	.	+	0	ID=BBa_K1150016;Name=BBa_K1150016
BBa_K1150057	.	tag	353	379	.	+	0	ID=annotation2347937;Name=HA;Parent=BBa_K1150016
BBa_K1150057	.	CDS	4190	4198	.	+	0	ID=BBa_K1150013;Name=BBa_K1150013
BBa_K1150057	.	sequence_feature	4190	4198	.	+	0	ID=annotation2347934;Name=AGS Linker;Parent=BBa_K1150013
BBa_K1150057	.	engineered_region	4587	4607	.	+	0	ID=BBa_K1150010;Name=BBa_K1150010
BBa_K1150057	.	CDS	4587	4607	.	+	0	ID=annotation2347465;Name=NLS;Parent=BBa_K1150010
>BBa_K1150057
ctgtggaatgtgtgtcagttagggtgtggaaagtccccaggctccccagcaggcagaagtatgcaaagcatgcatctca
attagtcagcaaccaggtgtggaaagtccccaggctccccagcaggcagaagtatgcaaagcatgcatctcaattagtc
agcaaccatagtcccgcccctaactccgcccatcccgcccctaactccgcccagttccgcccattctccgccccatggc
tgactaattttttttatttatgcagaggccgaggccgcctcggcctctgagctattccagaagtagtgaggaggctttt
ttggaggcctaggcttttgcaaaaagcttactagagtacccatacgatgttccagattacgcttactagagcctgcaag
aaaaagattgaattactagaggacaagaagtacagcatcggcctggccatcggcaccaactctgtgggctgggccgtga
tcaccgacgagtacaaggtgcccagcaagaaattcaaggtgctgggcaacaccgaccggcacagcatcaagaagaacct
gatcggagccctgctgttcgacagcggcgaaacagccgaggccacccggctgaagagaaccgccagaagaagatacacc
agacggaagaaccggatctgctatctgcaagagatcttcagcaacgagatggccaaggtggacgacagcttcttccaca
gactggaagagtccttcctggtggaagaggataagaagcacgagcggcaccccatcttcggcaacatcgtggacgaggt
ggcctaccacgagaagtaccccaccatctaccacctgagaaagaaactggtggacagcaccgacaaggccgacctgcgg
ctgatctatctggccctggcccacatgatcaagttccggggccacttcctgatcgagggcgacctgaaccccgacaaca
gcgacgtggacaagctgttcatccagctggtgcagacctacaaccagctgttcgaggaaaaccccatcaacgccagcgg
cgtggacgccaaggccatcctgtctgccagactgagcaagagcagacggctggaaaatctgatcgcccagctgcccggc
gagaagaagaatggcctgttcggcaacctgattgccctgagcctgggcctgacccccaacttcaagagcaacttcgacc
tggccgaggatgccaaacttcagctgagcaaggacacctacgacgacgacctggacaacctgctggcccagatcggcga
ccagtacgccgacctgtttctggccgccaagaacctgtccgacgccatcctgctgagcgacatcctgagagtgaacacc
gagatcaccaaggcccccctgagcgcctctatgatcaagagatacgacgagcaccaccaggacctgaccctgctgaaag
ctctcgtgcggcagcagctgcctgagaagtacaaagagattttcttcgaccagagcaagaacggctacgccggatacat
tgacggcggagccagccaggaagagttctacaagttcatcaagcccatcctggaaaagatggacggcaccgaggaactg
ctcgtgaagctgaacagagaggacctgctgcggaagcagcggaccttcgacaacggcagcatcccccaccagatccacc
tgggagagctgcacgccattctgcggcggcaggaagatttttacccattcctgaaggacaaccgggaaaagatcgagaa
gatcctgaccttccgcatcccctactacgtgggccctctggccaggggaaacagcagattcgcctggatgaccagaaag
agcgaggaaaccatcaccccctggaacttcgaggaagtggtggacaagggcgcttccgcccagagcttcatcgagcgga
tgaccaacttcgataagaacctgcccaacgagaaggtgctgcccaagcacagcctgctgtacgagtacttcaccgtgta
taacgagctgaccaaagtgaaatacgtgaccgagggaatgagaaagcccgccttcctgagcggcgagcagaaaaaggcc
atcgtggacctgctgttcaagaccaaccggaaagtgaccgtgaagcagctgaaagaggactacttcaagaaaatcgagt
gcttcgactccgtggaaatctccggcgtggaagatcggttcaacgcctccctgggcacataccacgatctgctgaaaat
tatcaaggacaaggacttcctggacaatgaggaaaacgaggacattctggaagatatcgtgctgaccctgacactgttt
gaggacagagagatgatcgaggaacggctgaaaacctatgcccacctgttcgacgacaaagtgatgaagcagctgaagc
ggcggagatacaccggctggggcaggctgagccggaagctgatcaacggcatccgggacaagcagtccggcaagacaat
cctggatttcctgaagtccgacggcttcgccaacagaaacttcatgcagctgatccacgacgacagcctgacctttaaa
gaggacatccagaaagcccaggtgtccggccagggcgatagcctgcacgagcacattgccaatctggcaggcagccccg
ccattaagaagggcatcctgcaaacagtgaaggtggtggacgagctcgtgaaagtgatgggacggcacaagcccgagaa
catcgtgatcgaaatggccagagagaaccagaccacccagaagggacagaagaacagccgcgagagaatgaagcggatc
gaagagggcatcaaagagctgggcagccagatcctgaaagaacaccccgtggaaaacacccagcttcagaacgagaagc
tgtacctgtactacctgcaaaatgggcgggatatgtacgtggaccaggaactggacatcaaccggctgtccgactacga
tgtggacgctatcgtgcctcagagctttctgaaggacgactccatcgacaacaaggtgctgaccagaagcgacaagaac
cggggcaagagcgacaacgtgccctccgaagaggtcgtgaagaagatgaagaactactggcggcagctgctgaacgcca
agctgattacccagagaaagttcgacaatctgaccaaggccgagagaggcggcctgagcgaactggataaggccggatt
catcaagagacagctggtggaaacccggcagatcacaaagcacgtggcacagatcctggactcccggatgaacactaag
tacgacgagaatgacaagctgatccgggaagtgaaagtgatcaccctgaagtccaagctggtgtccgatttccggaagg
atttccagttttacaaagtgcgcgagatcaacaactaccaccacgcccacgacgcctacctgaacgccgtcgtgggaac
cgccctgatcaaaaagtaccctaagctggaaagcgagttcgtgtacggcgactacaaggtgtacgacgtgcggaagatg
atcgccaagagcgagcaggaaatcggcaaggctaccgccaagtacttcttctacagcaacatcatgaactttttcaaga
ccgagattaccctggccaacggcgagatccggaagcggcctctgatcgagacaaacggcgaaaccggggagatcgtgtg
ggataagggccgggattttgccaccgtgcggaaagtgctgagcatgccccaagtgaatatcgtgaaaaagaccgaggtg
cagacaggcggcttcagcaaagagtctatcctgcccaagaggaacagcgataagctgatcgccagaaagaaggactggg
accctaagaagtacggcggcttcgacagccccaccgtggcctattctgtgctggtggtggccaaagtggaaaagggcaa
gtccaagaaactgaagagtgtgaaagagctgctggggatcaccatcatggaaagaagcagcttcgagaagaatcccatc
gactttctggaagccaagggctacaaagaagtgaaaaaggacctgatcatcaagctgcctaagtactccctgttcgagc
tggaaaacggccggaagagaatgctggcctctgcgggcgaactgcaaaagggaaacgaactggccctgcccgtgctgga
cgccaccctgatccaccagagcatcaccggcctgtacgagacacggatcgacctgtctcagctgggaggcgactactag
aggcaggctcctactagaggcgcgtacgaaaaacaattacgggtctaccatcgagggcctgctcgatctcccggacgac
gacgcccccgaagaggcggggctggcggctccgcgcctgtcctttctccccgcgggacacacgcgcagactgtcgacgg
cccccccgaccgatgtcagcctgggggacgagctccacttagacggcgaggacgtggcgatggcgcatgccgacgcgct
agacgatttcgatctggacatgttgggggacggggattccccgggtccgggatttaccccccacgactccgccccctac
ggcgctctggatatggccgacttcgagtttgagcagatgtttaccgatgcccttggaattgacgagtacggtgggtact
agagcctgcaagaaaaagattgaattactagaggtgccttctagttgccagccatctgttgtttgcccctcccccgtgc
cttccttgaccctggaaggtgccactcccactgtcctttcctaataaaatgaggaaattgcatcgcattgtctgagtag
gtgtcattctattctggggggtggggtggggcaggacagcaagggggaggattgggaagacaatagcaggcatgctggg
gatgcggtgggctctatg