LOCUS BBa_K1150058 4867 bp DNA linear 14-Nov-2024 DEFINITION Truncated dCas9 VP16 Device #2 ACCESSION BBa_K1150058 VERSION BBa_K1150058.1 FEATURES Location/Qualifiers promoter 1..344 /label=SV40 promoter 1..344 /label=BBa_K1150011 misc_feature 353..379 /label=HA misc_feature 353..379 /label=BBa_K1150016 CDS 388..408 /label=NLS misc_feature 388..408 /label=BBa_K1150010 CDS 417..4211 /label=BBa_K1150065 misc_feature 4220..4228 /label=AGS Linker CDS 4220..4228 /label=BBa_K1150013 misc_feature 4237..4608 /label=VP16 CDS 4237..4608 /label=BBa_K1150001 CDS 4617..4637 /label=NLS misc_feature 4617..4637 /label=BBa_K1150010 misc_feature 4646..4867 /label=BGH terminator 4646..4867 /label=BBa_K1150012 ORIGIN 1 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt 61 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca 121 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta 181 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga 241 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag 301 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agcttactag agtacccata 361 cgatgttcca gattacgctt actagagcct gcaagaaaaa gattgaatta ctagaggaca 421 agaagtacag catcggcctg gccatcggca ccaactctgt gggctgggcc gtgatcaccg 481 acgagtacaa ggtgcccagc aagaaattca aggtgctggg caacaccgac cggcacagca 541 tcaagaagaa cctgatcgga gccctgctgt tcgacagcgg cgaaacagcc gaggccaccc 601 ggctgaagag aaccgccaga agaagataca ccagacggaa gaaccggatc tgctatctgc 661 aagagatctt cagcaacgag atggccaagg tggacgacag cttcttccac agactggaag 721 agtccttcct ggtggaagag gataagaagc acgagcggca ccccatcttc ggcaacatcg 781 tggacgaggt ggcctaccac gagaagtacc ccaccatcta ccacctgaga aagaaactgg 841 tggacagcac cgacaaggcc gacctgcggc tgatctatct ggccctggcc cacatgatca 901 agttccgggg cgacacctac gacgacgacc tggacaacct gctggcccag atcggcgacc 961 agtacgccga cctgtttctg gccgccaaga acctgtccga cgccatcctg ctgagcgaca 1021 tcctgagagt gaacaccgag atcaccaagg cccccctgag cgcctctatg atcaagagat 1081 acgacgagca ccaccaggac ctgaccctgc tgaaagctct cgtgcggcag cagctgcctg 1141 agaagtacaa agagattttc ttcgaccaga gcaagaacgg ctacgccgga tacattgacg 1201 gcggagccag ccaggaagag ttctacaagt tcatcaagcc catcctggaa aagatggacg 1261 gcaccgagga actgctcgtg aagctgaaca gagaggacct gctgcggaag cagcggacct 1321 tcgacaacgg cagcatcccc caccagatcc acctgggaga gctgcacgcc attctgcggc 1381 ggcaggaaga tttttaccca ttcctgaagg acaaccggga aaagatcgag aagatcctga 1441 ccttccgcat cccctactac gtgggccctc tggccagggg aaacagcaga ttcgcctgga 1501 tgaccagaaa gagcgaggaa accatcaccc cctggaactt cgaggaagtg gtggacaagg 1561 gcgcttccgc ccagagcttc atcgagcgga tgaccaactt cgataagaac ctgcccaacg 1621 agaaggtgct gcccaagcac agcctgctgt acgagtactt caccgtgtat aacgagctga 1681 ccaaagtgaa atacgtgacc gagggaatga gaaagcccgc cttcctgagc ggcgagcaga 1741 aaaaggccat cgtggacctg ctgttcaaga ccaaccggaa agtgaccgtg aagcagctga 1801 aagaggacta cttcaagaaa atcgagtgct tcgactccgt ggaaatctcc ggcgtggaag 1861 atcggttcaa cgcctccctg ggcacatacc acgatctgct gaaaattatc aaggacaagg 1921 acttcctgga caatgaggaa aacgaggaca ttctggaaga tatcgtgctg accctgacac 1981 tgtttgagga cagagagatg atcgaggaac ggctgaaaac ctatgcccac ctgttcgacg 2041 acaaagtgat gaagcagctg aagcggcgga gatacaccgg ctggggcagg ctgagccgga 2101 agctgatcaa cggcatccgg gacaagcagt ccggcaagac aatcctggat ttcctgaagt 2161 ccgacggctt cgccaacaga aacttcatgc agctgatcca cgacgacagc ctgaccttta 2221 aagaggacat ccagaaagcc caggtgtccg gccagggcga tagcctgcac gagcacattg 2281 ccaatctggc aggcagcccc gccattaaga agggcatcct gcaaacagtg aaggtggtgg 2341 acgagctcgt gaaagtgatg ggacggcaca agcccgagaa catcgtgatc gaaatggcca 2401 gagagaacca gaccacccag aagggacaga agaacagccg cgagagaatg aagcggatcg 2461 aagagggcat caaagagctg ggcagccaga tcctgaaaga acaccccgtg gaaaacaccc 2521 agcttcagaa cgagaagctg tacctgtact acctgcaaaa tgggcgggat atgtacgtgg 2581 accaggaact ggacatcaac cggctgtccg actacgatgt ggacgctatc gtgcctcaga 2641 gctttctgaa ggacgactcc atcgacaaca aggtgctgac cagaagcgac aagaaccggg 2701 gcaagagcga caacgtgccc tccgaagagg tcgtgaagaa gatgaagaac tactggcggc 2761 agctgctgaa cgccaagctg attacccaga gaaagttcga caatctgacc aaggccgaga 2821 gaggcggcct gagcgaactg gataaggccg gattcatcaa gagacagctg gtggaaaccc 2881 ggcagatcac aaagcacgtg gcacagatcc tggactcccg gatgaacact aagtacgacg 2941 agaatgacaa gctgatccgg gaagtgaaag tgatcaccct gaagtccaag ctggtgtccg 3001 atttccggaa ggatttccag ttttacaaag tgcgcgagat caacaactac caccacgccc 3061 acgacgccta cctgaacgcc gtcgtgggaa ccgccctgat caaaaagtac cctaagctgg 3121 aaagcgagtt cgtgtacggc gactacaagg tgtacgacgt gcggaagatg atcgccaaga 3181 gcgagcagga aatcggcaag gctaccgcca agtacttctt ctacagcaac atcatgaact 3241 ttttcaagac cgagattacc ctggccaacg gcgagatccg gaagcggcct ctgatcgaga 3301 caaacggcga aaccggggag atcgtgtggg ataagggccg ggattttgcc accgtgcgga 3361 aagtgctgag catgccccaa gtgaatatcg tgaaaaagac cgaggtgcag acaggcggct 3421 tcagcaaaga gtctatcctg cccaagagga acagcgataa gctgatcgcc agaaagaagg 3481 actgggaccc taagaagtac ggcggcttcg acagccccac cgtggcctat tctgtgctgg 3541 tggtggccaa agtggaaaag ggcaagtcca agaaactgaa gagtgtgaaa gagctgctgg 3601 ggatcaccat catggaaaga agcagcttcg agaagaatcc catcgacttt ctggaagcca 3661 agggctacaa agaagtgaaa aaggacctga tcatcaagct gcctaagtac tccctgttcg 3721 agctggaaaa cggccggaag agaatgctgg cctctgcggg cgaactgcaa aagggaaacg 3781 aactggccct gccctccaaa tatgtgaact tcctgtacct ggccagccac tatgagaagc 3841 tgaagggctc ccccgaggat aatgagcaga aacagctgtt tgtggaacag cacaagcact 3901 acctggacga gatcatcgag cagatcagcg agttctccaa gagagtgatc ctggccgacg 3961 ctaatctgga caaagtgctg tccgcctaca acaagcaccg ggataagccc atcagagagc 4021 aggccgagaa tatcatccac ctgtttaccc tgaccaatct gggagcccct gccgccttca 4081 agtactttga caccaccatc gaccggaaga ggtacaccag caccaaagag gtgctggacg 4141 ccaccctgat ccaccagagc atcaccggcc tgtacgagac acggatcgac ctgtctcagc 4201 tgggaggcga ctactagagg caggctccta ctagaggcgc gtacgaaaaa caattacggg 4261 tctaccatcg agggcctgct cgatctcccg gacgacgacg cccccgaaga ggcggggctg 4321 gcggctccgc gcctgtcctt tctccccgcg ggacacacgc gcagactgtc gacggccccc 4381 ccgaccgatg tcagcctggg ggacgagctc cacttagacg gcgaggacgt ggcgatggcg 4441 catgccgacg cgctagacga tttcgatctg gacatgttgg gggacgggga ttccccgggt 4501 ccgggattta ccccccacga ctccgccccc tacggcgctc tggatatggc cgacttcgag 4561 tttgagcaga tgtttaccga tgcccttgga attgacgagt acggtgggta ctagagcctg 4621 caagaaaaag attgaattac tagaggtgcc ttctagttgc cagccatctg ttgtttgccc 4681 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa 4741 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg 4801 gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg 4861 ctctatg //