LOCUS BBa_K1150059 4042 bp DNA linear 14-Nov-2024 DEFINITION Truncated dCas9 VP16 Device #3 ACCESSION BBa_K1150059 VERSION BBa_K1150059.1 FEATURES Location/Qualifiers promoter 1..344 /label=SV40 promoter 1..344 /label=BBa_K1150011 misc_feature 353..379 /label=HA misc_feature 353..379 /label=BBa_K1150016 CDS 388..408 /label=NLS misc_feature 388..408 /label=BBa_K1150010 CDS 417..3386 /label=BBa_K1150066 misc_feature 3395..3403 /label=AGS Linker CDS 3395..3403 /label=BBa_K1150013 misc_feature 3412..3783 /label=VP16 CDS 3412..3783 /label=BBa_K1150001 CDS 3792..3812 /label=NLS misc_feature 3792..3812 /label=BBa_K1150010 misc_feature 3821..4042 /label=BGH terminator 3821..4042 /label=BBa_K1150012 ORIGIN 1 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt 61 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca 121 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta 181 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga 241 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag 301 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agcttactag agtacccata 361 cgatgttcca gattacgctt actagagcct gcaagaaaaa gattgaatta ctagaggaca 421 agaagtacag catcggcctg gccatcggcc tcgtgaagct gaacagagag gacctgctgc 481 ggaagcagcg gaccttcgac aacggcagca tcccccacca gatccacctg ggagagctgc 541 acgccattct gcggcggcag gaagattttt acccattcct gaaggacaac cgggaaaaga 601 tcgagaagat cctgaccttc cgcatcccct actacgtggg ccctctggcc aggggaaaca 661 gcagattcgc ctggatgacc agaaagagcg aggaaaccat caccccctgg aacttcgagg 721 aagtggtgga caagggcgct tccgcccaga gcttcatcga gcggatgacc aacttcgata 781 agaacctgcc caacgagaag gtgctgccca agcacagcct gctgtacgag tacttcaccg 841 tgtataacga gctgaccaaa gtgaaatacg tgaccgaggg aatgagaaag cccgccttcc 901 tgagcggcga gcagaaaaag gccatcgtgg acctgctgtt caagaccaac cggaaagtga 961 ccgtgaagca gctgaaagag gactacttca agaaaatcga gtgcttcgac tccgtggaaa 1021 tctccggcgt ggaagatcgg ttcaacgcct ccctgggcac ataccacgat ctgctgaaaa 1081 ttatcaagga caaggacttc ctggacaatg aggaaaacga ggacattctg gaagatatcg 1141 tgctgaccct gacactgttt gaggacagag agatgatcga ggaacggctg aaaacctatg 1201 cccacctgtt cgacgacaaa gtgatgaagc agctgaagcg gcggagatac accggctggg 1261 gcaggctgag ccggaagctg atcaacggca tccgggacaa gcagtccggc aagacaatcc 1321 tggatttcct gaagtccgac ggcttcgcca acagaaactt catgcagctg atccacgacg 1381 acagcctgac ctttaaagag gacatccaga aagcccaggt gtccggccag ggcgatagcc 1441 tgcacgagca cattgccaat ctggcaggca gccccgccat taagaagggc atcctgcaaa 1501 cagtgaaggt ggtggacgag ctcgtgaaag tgatgggacg gcacaagccc gagaacatcg 1561 tgatcgaaat ggccagagag aaccagacca cccagaaggg acagaagaac agccgcgaga 1621 gaatgaagcg gatcgaagag ggcatcaaag agctgggcag ccagatcctg aaagaacacc 1681 ccgtggaaaa cacccagctt cagaacgaga agctgtacct gtactacctg caaaatgggc 1741 gggatatgta cgtggaccag gaactggaca tcaaccggct gtccgactac gatgtggacg 1801 ctatcgtgcc tcagagcttt ctgaaggacg actccatcga caacaaggtg ctgaccagaa 1861 gcgacaagaa ccggggcaag agcgacaacg tgccctccga agaggtcgtg aagaagatga 1921 agaactactg gcggcagctg ctgaacgcca agctgattac ccagagaaag ttcgacaatc 1981 tgaccaaggc cgagagaggc ggcctgagcg aactggataa ggccggattc atcaagagac 2041 agctggtgga aacccggcag atcacaaagc acgtggcaca gatcctggac tcccggatga 2101 acactaagta cgacgagaat gacaagctga tccgggaagt gaaagtgatc accctgaagt 2161 ccaagctggt gtccgatttc cggaaggatt tccagtttta caaagtgcgc gagatcaaca 2221 actaccacca cgcccacgac gcctacctga acgccgtcgt gggaaccgcc ctgatcaaaa 2281 agtaccctaa gctggaaagc gagttcgtgt acggcgacta caaggtgtac gacgtgcgga 2341 agatgatcgc caagagcgag caggaaatcg gcaaggctac cgccaagtac ttcttctaca 2401 gcaacatcat gaactttttc aagaccgaga ttaccctggc caacggcgag atccggaagc 2461 ggcctctgat cgagacaaac ggcgaaaccg gggagatcgt gtgggataag ggccgggatt 2521 ttgccaccgt gcggaaagtg ctgagcatgc cccaagtgaa tatcgtgaaa aagaccgagg 2581 tgcagacagg cggcttcagc aaagagtcta tcctgcccaa gaggaacagc gataagctga 2641 tcgccagaaa gaaggactgg gaccctaaga agtacggcgg cttcgacagc cccaccgtgg 2701 cctattctgt gctggtggtg gccaaagtgg aaaagggcaa gtccaagaaa ctgaagagtg 2761 tgaaagagct gctggggatc accatcatgg aaagaagcag cttcgagaag aatcccatcg 2821 actttctgga agccaagggc tacaaagaag tgaaaaagga cctgatcatc aagctgccta 2881 agtactccct gttcgagctg gaaaacggcc ggaagagaat gctggcctct gcgggcgaac 2941 tgcaaaaggg aaacgaactg gccctgccct ccaaatatgt gaacttcctg tacctggcca 3001 gccactatga gaagctgaag ggctcccccg aggataatga gcagaaacag ctgtttgtgg 3061 aacagcacaa gcactacctg gacgagatca tcgagcagat cagcgagttc tccaagagag 3121 tgatcctggc cgacgctaat ctggacaaag tgctgtccgc ctacaacaag caccgggata 3181 agcccatcag agagcaggcc gagaatatca tccacctgtt taccctgacc aatctgggag 3241 cccctgccgc cttcaagtac tttgacacca ccatcgaccg gaagaggtac accagcacca 3301 aagaggtgct ggacgccacc ctgatccacc agagcatcac cggcctgtac gagacacgga 3361 tcgacctgtc tcagctggga ggcgactact agaggcaggc tcctactaga ggcgcgtacg 3421 aaaaacaatt acgggtctac catcgagggc ctgctcgatc tcccggacga cgacgccccc 3481 gaagaggcgg ggctggcggc tccgcgcctg tcctttctcc ccgcgggaca cacgcgcaga 3541 ctgtcgacgg cccccccgac cgatgtcagc ctgggggacg agctccactt agacggcgag 3601 gacgtggcga tggcgcatgc cgacgcgcta gacgatttcg atctggacat gttgggggac 3661 ggggattccc cgggtccggg atttaccccc cacgactccg ccccctacgg cgctctggat 3721 atggccgact tcgagtttga gcagatgttt accgatgccc ttggaattga cgagtacggt 3781 gggtactaga gcctgcaaga aaaagattga attactagag gtgccttcta gttgccagcc 3841 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt 3901 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct 3961 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc 4021 tggggatgcg gtgggctcta tg //