LOCUS BBa_K1150060 3787 bp DNA linear 27-Jan-2026 DEFINITION Truncated dCas9 VP16 Device #4 ACCESSION BBa_K1150060 VERSION BBa_K1150060.1 FEATURES Location/Qualifiers promoter 1..344 /label=SV40 promoter 1..344 /label=BBa_K1150011 misc_feature 353..379 /label=HA misc_feature 353..379 /label=BBa_K1150016 CDS 388..408 /label=NLS misc_feature 388..408 /label=BBa_K1150010 CDS 417..3131 /label=BBa_K1150067 misc_feature 3140..3148 /label=AGS Linker CDS 3140..3148 /label=BBa_K1150013 misc_feature 3157..3528 /label=VP16 CDS 3157..3528 /label=BBa_K1150001 CDS 3537..3557 /label=NLS misc_feature 3537..3557 /label=BBa_K1150010 misc_feature 3566..3787 /label=BGH terminator 3566..3787 /label=BBa_K1150012 ORIGIN 1 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt 61 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca 121 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta 181 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga 241 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag 301 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agcttactag agtacccata 361 cgatgttcca gattacgctt actagagcct gcaagaaaaa gattgaatta ctagaggaca 421 agaagtacag catcggcctg gccatcggca ccaactctgt gggctgggcc gtgatcaccg 481 acgagtacaa ggtgcccagc aagaaattca aggtgctggg caacaccgac cggcacagca 541 tcaagaagaa cctgatcgga gccctgctgt tcgacagcgg cgaaacagcc gaggccaccc 601 ggctgaagag aaccgccaga agaagataca ccagacggaa gaaccggatc tgctatctgc 661 aagagatctt cagcaacgag atggccaagg tggacgacag cttcttccac agactggaag 721 agtccttcct ggtggaagag gataagaagc acgagcggca ccccatcttc ggcaacatcg 781 tggacgaggt ggcctaccac gagaagtacc ccaccatcta ccacctgaga aagaaactgg 841 tggacagcac cgacaaggcc gacctgcggc tgatctatct ggccctggcc cacatgatca 901 agttccgggg ccacttcctg atcgagggcg acctgaaccc cgacaacagc gacgtggaca 961 agctgttcat ccagctggtg cagacctaca accagctgtt cgaggaaaac cccatcaacg 1021 ccagcggcgt ggacgccaag gccatcctgt ctgccagact gagcaagagc agacggctgg 1081 aaaatctgat cgcccagctg cccggcgaga agaagaatgg cctgttcggc aacctgattg 1141 ccctgagcct gggcctgacc cccaacttca agagcaactt cgacctggcc gaggatgcca 1201 aacttcagct gagcaaggac acctacgacg acgacctgga caacctgctg gcccagatcg 1261 gcgaccagta cgccgacctg tttctggccg ccaagaacct gtccgacgcc atcctgctga 1321 gcgacatcct gagagtgaac accgagatca ccaaggcccc cctgagcgcc tctatgatca 1381 agagatacga cgagcaccac caggacctga ccctgctgaa agctctcgtg cggcagcagc 1441 tgcctgagaa gtacaaagag attttcttcg accagagcaa gaacggctac gccggataca 1501 ttgacggcgg agccagccag gaagagttct acaagttcat caagcccatc ctggaaaaga 1561 tggacggcac cgaggaactg ctcgtgaagc tgaacagaga ggacctgctg cggaagcagc 1621 ggaccttcga caacggcagc atcccccacc agatccacct gggagagctg cacgccattc 1681 tgcggcggca ggccaagctg attacccaga gaaagttcga caatctgacc aaggccgaga 1741 gaggcggcct gagcgaactg gataaggccg gattcatcaa gagacagctg gtggaaaccc 1801 ggcagatcac aaagcacgtg gcacagatcc tggactcccg gatgaacact aagtacgacg 1861 agaatgacaa gctgatccgg gaagtgaaag tgatcaccct gaagtccaag ctggtgtccg 1921 atttccggaa ggatttccag ttttacaaag tgcgcgagat caacaactac caccacgccc 1981 acgacgccta cctgaacgcc gtcgtgggaa ccgccctgat caaaaagtac cctaagctgg 2041 aaagcgagtt cgtgtacggc gactacaagg tgtacgacgt gcggaagatg atcgccaaga 2101 gcgagcagga aatcggcaag gctaccgcca agtacttctt ctacagcaac atcatgaact 2161 ttttcaagac cgagattacc ctggccaacg gcgagatccg gaagcggcct ctgatcgaga 2221 caaacggcga aaccggggag atcgtgtggg ataagggccg ggattttgcc accgtgcgga 2281 aagtgctgag catgccccaa gtgaatatcg tgaaaaagac cgaggtgcag acaggcggct 2341 tcagcaaaga gtctatcctg cccaagagga acagcgataa gctgatcgcc agaaagaagg 2401 actgggaccc taagaagtac ggcggcttcg acagccccac cgtggcctat tctgtgctgg 2461 tggtggccaa agtggaaaag ggcaagtcca agaaactgaa gagtgtgaaa gagctgctgg 2521 ggatcaccat catggaaaga agcagcttcg agaagaatcc catcgacttt ctggaagcca 2581 agggctacaa agaagtgaaa aaggacctga tcatcaagct gcctaagtac tccctgttcg 2641 agctggaaaa cggccggaag agaatgctgg cctctgcggg cgaactgcaa aagggaaacg 2701 aactggccct gccctccaaa tatgtgaact tcctgtacct ggccagccac tatgagaagc 2761 tgaagggctc ccccgaggat aatgagcaga aacagctgtt tgtggaacag cacaagcact 2821 acctggacga gatcatcgag cagatcagcg agttctccaa gagagtgatc ctggccgacg 2881 ctaatctgga caaagtgctg tccgcctaca acaagcaccg ggataagccc atcagagagc 2941 aggccgagaa tatcatccac ctgtttaccc tgaccaatct gggagcccct gccgccttca 3001 agtactttga caccaccatc gaccggaaga ggtacaccag caccaaagag gtgctggacg 3061 ccaccctgat ccaccagagc atcaccggcc tgtacgagac acggatcgac ctgtctcagc 3121 tgggaggcga ctactagagg caggctccta ctagaggcgc gtacgaaaaa caattacggg 3181 tctaccatcg agggcctgct cgatctcccg gacgacgacg cccccgaaga ggcggggctg 3241 gcggctccgc gcctgtcctt tctccccgcg ggacacacgc gcagactgtc gacggccccc 3301 ccgaccgatg tcagcctggg ggacgagctc cacttagacg gcgaggacgt ggcgatggcg 3361 catgccgacg cgctagacga tttcgatctg gacatgttgg gggacgggga ttccccgggt 3421 ccgggattta ccccccacga ctccgccccc tacggcgctc tggatatggc cgacttcgag 3481 tttgagcaga tgtttaccga tgcccttgga attgacgagt acggtgggta ctagagcctg 3541 caagaaaaag attgaattac tagaggtgcc ttctagttgc cagccatctg ttgtttgccc 3601 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa 3661 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg 3721 gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg 3781 ctctatg //