LOCUS BBa_K1150061 3883 bp DNA linear 22-Dec-2024 DEFINITION Truncated dCas9 VP16 Device #5 ACCESSION BBa_K1150061 VERSION BBa_K1150061.1 FEATURES Location/Qualifiers promoter 1..344 /label=SV40 promoter 1..344 /label=BBa_K1150011 misc_feature 353..379 /label=HA misc_feature 353..379 /label=BBa_K1150016 CDS 388..408 /label=NLS misc_feature 388..408 /label=BBa_K1150010 CDS 417..3227 /label=BBa_K1150068 misc_feature 3236..3244 /label=AGS Linker CDS 3236..3244 /label=BBa_K1150013 misc_feature 3253..3624 /label=VP16 CDS 3253..3624 /label=BBa_K1150001 CDS 3633..3653 /label=NLS misc_feature 3633..3653 /label=BBa_K1150010 misc_feature 3662..3883 /label=BGH terminator 3662..3883 /label=BBa_K1150012 ORIGIN 1 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt 61 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca 121 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta 181 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga 241 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag 301 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agcttactag agtacccata 361 cgatgttcca gattacgctt actagagcct gcaagaaaaa gattgaatta ctagaggaca 421 agaagtacag catcggcctg gccatcggca ccaactctgt gggctgggcc gtgatcaccg 481 acgagtacaa ggtgcccagc aagaaattca aggtgctggg caacaccgac cggcacagca 541 tcaagaagaa cctgatcgga gccctgctgt tcgacagcgg cgaaacagcc gaggccaccc 601 ggctgaagag aaccgccaga agaagataca ccagacggaa gaaccggatc tgctatctgc 661 aagagatctt cagcaacgag atggccaagg tggacgacag cttcttccac agactggaag 721 agtccttcct ggtggaagag gataagaagc acgagcggca ccccatcttc ggcaacatcg 781 tggacgaggt ggcctaccac gagaagtacc ccaccatcta ccacctgaga aagaaactgg 841 tggacagcac cgacaaggcc gacctgcggc tgatctatct ggccctggcc cacatgatca 901 agttccgggg ccacttcctg atcgagggcg acctgaaccc cgacaacagc gacgtggaca 961 agctgttcat ccagctggtg cagacctaca accagctgtt cgaggaaaac cccatcaacg 1021 ccagcggcgt ggacgccaag gccatcctgt ctgccagact gagcaagagc agacggctgg 1081 aaaatctgat cgcccagctg cccggcgaga agaagaatgg cctgttcggc aacctgattg 1141 ccctgagcct gggcctgacc cccaacttca agagcaactt cgacctggcc gaggatgcca 1201 aacttcagct gagcaaggac acctacgacg acgacctgga caacctgctg gcccagatcg 1261 gcgaccagta cgccgacctg tttctggccg ccaagaacct gtccgacgcc atcctgctga 1321 gcgacatcct gagagtgaac accgagatca ccaaggcccc cctgagcgcc tctatgatca 1381 agagatacga cgagcaccac caggacctga ccctgctgaa agctctcgtg cggcagcagc 1441 tgcctgagaa gtacaaagag attttcttcg accagagcaa gaacggctac gccggataca 1501 ttgacggcgg agccagccag gaagagttct acaagttcat caagcccatc ctggaaaaga 1561 tggacggcac cgaggaactg ctcgtgaagc tgaacagaga ggacctgctg cggaagcagc 1621 ggaccttcga caacggcagc atcccccacc agatccacct gggagagctg cacgccattc 1681 tgcggcggca ggaagatttt tacccattcc tgaaggacaa ccgggaaaag atcgagaaga 1741 tcctgacctt ccgcatcccc tactacgtgg gccctctggc caggggaaac agcagattcg 1801 cctggatgac cagaaagagc gaggaaacca tcaccccctg gaacttcgag gaagtggtgg 1861 acaagggcgc ttccgcccag agcttcatcg agcggatgac caacttcgat aagaacctgc 1921 ccaacgagaa ggtgctgccc aagcacagcc tgctgtacga gtacttcacc gtgtataacg 1981 agctgaccaa agtgaaatac gtgaccgagg gaatgagaaa gcccgccttc ctgagcggcg 2041 agcagaaaaa ggccatcgtg gacctgctgt tcaagaccaa ccggaaagtg accgtgaagc 2101 agctgaaaga ggactacttc aagaaaatcg agtgcttcga ctccgtggaa atctccggcg 2161 tggaagatcg gttcaacgcc tccctgggca cataccacga tctgctgaaa attatcaagg 2221 acaaggactt cctggacaat gaggaaaacg aggacattct ggaagatatc gtgctgaccc 2281 tgacactgtt tgaggacaga gagatgatcg aggaacggct gaaaacctat gcccacctgt 2341 tcgacgacaa agtgatgaag cagctgaagc ggcggagata caccggctgg ggcaggctga 2401 gccggaagct gatcaacggc atccgggaca agcagtccgg caagacaatc ctggatttcc 2461 tgaagtccga cggcttcgcc aacagaaact tcatgcagct gatccacgac gacagcctga 2521 cctttaaaga ggacatccag aaagcccagg tgtccggcca gggcgatagc ctgcacgagc 2581 acattgccaa tctggcaggc agccccgcca ttaagaaggg catcctgcaa acagtgaagg 2641 tggtggacga gctcgtgaaa gtgatgggac ggcacaagcc cgagaacatc gtgatcgaaa 2701 tggccagaga gaaccagacc acccagaagg gacagaagaa cagccgcgag agaatgaagc 2761 ggatcgaaga gggcatcaaa gagctgggca gccagatcct gaaagaacac cccgtggaaa 2821 acacccagct tcagaacgag aagctgtacc tgtactacct gcaaaatggg cgggatatgt 2881 acgtggacca ggaactggac atcaaccggc tgtccgacta cgatgtggac gctatcgtgc 2941 ctcagagctt tctgaaggac gactccatcg acaacaaggt gctgaccaga agcgacaaga 3001 accggggcaa gagcgacaac gtgccctccg aagaggtcgt gaagaagatg aagaactact 3061 ggcggcagct gctgaacgcc aagctgatta cccagagaaa gttcgacaat ctgaccaagg 3121 ccgagagagg cggcctgagc gaactggtgc tggacgccac cctgatccac cagagcatca 3181 ccggcctgta cgagacacgg atcgacctgt ctcagctggg aggcgactac tagaggcagg 3241 ctcctactag aggcgcgtac gaaaaacaat tacgggtcta ccatcgaggg cctgctcgat 3301 ctcccggacg acgacgcccc cgaagaggcg gggctggcgg ctccgcgcct gtcctttctc 3361 cccgcgggac acacgcgcag actgtcgacg gcccccccga ccgatgtcag cctgggggac 3421 gagctccact tagacggcga ggacgtggcg atggcgcatg ccgacgcgct agacgatttc 3481 gatctggaca tgttggggga cggggattcc ccgggtccgg gatttacccc ccacgactcc 3541 gccccctacg gcgctctgga tatggccgac ttcgagtttg agcagatgtt taccgatgcc 3601 cttggaattg acgagtacgg tgggtactag agcctgcaag aaaaagattg aattactaga 3661 ggtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct 3721 ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct 3781 gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg 3841 ggaagacaat agcaggcatg ctggggatgc ggtgggctct atg //