LOCUS BBa_K1150063 4182 bp DNA linear 14-Nov-2024 DEFINITION HA-NLS-Cas9-Linker 3 AA ACCESSION BBa_K1150063 VERSION BBa_K1150063.1 FEATURES Location/Qualifiers misc_feature 1..27 /label=HA misc_feature 1..27 /label=BBa_K1150016 CDS 36..56 /label=NLS misc_feature 36..56 /label=BBa_K1150010 CDS 65..4165 /label=dCas9 misc_feature 2579..2581 /label=Nickaseless mutation CDS 65..4165 /label=BBa_K1150000 misc_feature 4174..4182 /label=AGS Linker CDS 4174..4182 /label=BBa_K1150013 ORIGIN 1 tacccatacg atgttccaga ttacgcttac tagagcctgc aagaaaaaga ttgaattact 61 agaggacaag aagtacagca tcggcctggc catcggcacc aactctgtgg gctgggccgt 121 gatcaccgac gagtacaagg tgcccagcaa gaaattcaag gtgctgggca acaccgaccg 181 gcacagcatc aagaagaacc tgatcggagc cctgctgttc gacagcggcg aaacagccga 241 ggccacccgg ctgaagagaa ccgccagaag aagatacacc agacggaaga accggatctg 301 ctatctgcaa gagatcttca gcaacgagat ggccaaggtg gacgacagct tcttccacag 361 actggaagag tccttcctgg tggaagagga taagaagcac gagcggcacc ccatcttcgg 421 caacatcgtg gacgaggtgg cctaccacga gaagtacccc accatctacc acctgagaaa 481 gaaactggtg gacagcaccg acaaggccga cctgcggctg atctatctgg ccctggccca 541 catgatcaag ttccggggcc acttcctgat cgagggcgac ctgaaccccg acaacagcga 601 cgtggacaag ctgttcatcc agctggtgca gacctacaac cagctgttcg aggaaaaccc 661 catcaacgcc agcggcgtgg acgccaaggc catcctgtct gccagactga gcaagagcag 721 acggctggaa aatctgatcg cccagctgcc cggcgagaag aagaatggcc tgttcggcaa 781 cctgattgcc ctgagcctgg gcctgacccc caacttcaag agcaacttcg acctggccga 841 ggatgccaaa cttcagctga gcaaggacac ctacgacgac gacctggaca acctgctggc 901 ccagatcggc gaccagtacg ccgacctgtt tctggccgcc aagaacctgt ccgacgccat 961 cctgctgagc gacatcctga gagtgaacac cgagatcacc aaggcccccc tgagcgcctc 1021 tatgatcaag agatacgacg agcaccacca ggacctgacc ctgctgaaag ctctcgtgcg 1081 gcagcagctg cctgagaagt acaaagagat tttcttcgac cagagcaaga acggctacgc 1141 cggatacatt gacggcggag ccagccagga agagttctac aagttcatca agcccatcct 1201 ggaaaagatg gacggcaccg aggaactgct cgtgaagctg aacagagagg acctgctgcg 1261 gaagcagcgg accttcgaca acggcagcat cccccaccag atccacctgg gagagctgca 1321 cgccattctg cggcggcagg aagattttta cccattcctg aaggacaacc gggaaaagat 1381 cgagaagatc ctgaccttcc gcatccccta ctacgtgggc cctctggcca ggggaaacag 1441 cagattcgcc tggatgacca gaaagagcga ggaaaccatc accccctgga acttcgagga 1501 agtggtggac aagggcgctt ccgcccagag cttcatcgag cggatgacca acttcgataa 1561 gaacctgccc aacgagaagg tgctgcccaa gcacagcctg ctgtacgagt acttcaccgt 1621 gtataacgag ctgaccaaag tgaaatacgt gaccgaggga atgagaaagc ccgccttcct 1681 gagcggcgag cagaaaaagg ccatcgtgga cctgctgttc aagaccaacc ggaaagtgac 1741 cgtgaagcag ctgaaagagg actacttcaa gaaaatcgag tgcttcgact ccgtggaaat 1801 ctccggcgtg gaagatcggt tcaacgcctc cctgggcaca taccacgatc tgctgaaaat 1861 tatcaaggac aaggacttcc tggacaatga ggaaaacgag gacattctgg aagatatcgt 1921 gctgaccctg acactgtttg aggacagaga gatgatcgag gaacggctga aaacctatgc 1981 ccacctgttc gacgacaaag tgatgaagca gctgaagcgg cggagataca ccggctgggg 2041 caggctgagc cggaagctga tcaacggcat ccgggacaag cagtccggca agacaatcct 2101 ggatttcctg aagtccgacg gcttcgccaa cagaaacttc atgcagctga tccacgacga 2161 cagcctgacc tttaaagagg acatccagaa agcccaggtg tccggccagg gcgatagcct 2221 gcacgagcac attgccaatc tggcaggcag ccccgccatt aagaagggca tcctgcaaac 2281 agtgaaggtg gtggacgagc tcgtgaaagt gatgggacgg cacaagcccg agaacatcgt 2341 gatcgaaatg gccagagaga accagaccac ccagaaggga cagaagaaca gccgcgagag 2401 aatgaagcgg atcgaagagg gcatcaaaga gctgggcagc cagatcctga aagaacaccc 2461 cgtggaaaac acccagcttc agaacgagaa gctgtacctg tactacctgc aaaatgggcg 2521 ggatatgtac gtggaccagg aactggacat caaccggctg tccgactacg atgtggacgc 2581 tatcgtgcct cagagctttc tgaaggacga ctccatcgac aacaaggtgc tgaccagaag 2641 cgacaagaac cggggcaaga gcgacaacgt gccctccgaa gaggtcgtga agaagatgaa 2701 gaactactgg cggcagctgc tgaacgccaa gctgattacc cagagaaagt tcgacaatct 2761 gaccaaggcc gagagaggcg gcctgagcga actggataag gccggattca tcaagagaca 2821 gctggtggaa acccggcaga tcacaaagca cgtggcacag atcctggact cccggatgaa 2881 cactaagtac gacgagaatg acaagctgat ccgggaagtg aaagtgatca ccctgaagtc 2941 caagctggtg tccgatttcc ggaaggattt ccagttttac aaagtgcgcg agatcaacaa 3001 ctaccaccac gcccacgacg cctacctgaa cgccgtcgtg ggaaccgccc tgatcaaaaa 3061 gtaccctaag ctggaaagcg agttcgtgta cggcgactac aaggtgtacg acgtgcggaa 3121 gatgatcgcc aagagcgagc aggaaatcgg caaggctacc gccaagtact tcttctacag 3181 caacatcatg aactttttca agaccgagat taccctggcc aacggcgaga tccggaagcg 3241 gcctctgatc gagacaaacg gcgaaaccgg ggagatcgtg tgggataagg gccgggattt 3301 tgccaccgtg cggaaagtgc tgagcatgcc ccaagtgaat atcgtgaaaa agaccgaggt 3361 gcagacaggc ggcttcagca aagagtctat cctgcccaag aggaacagcg ataagctgat 3421 cgccagaaag aaggactggg accctaagaa gtacggcggc ttcgacagcc ccaccgtggc 3481 ctattctgtg ctggtggtgg ccaaagtgga aaagggcaag tccaagaaac tgaagagtgt 3541 gaaagagctg ctggggatca ccatcatgga aagaagcagc ttcgagaaga atcccatcga 3601 ctttctggaa gccaagggct acaaagaagt gaaaaaggac ctgatcatca agctgcctaa 3661 gtactccctg ttcgagctgg aaaacggccg gaagagaatg ctggcctctg cgggcgaact 3721 gcaaaaggga aacgaactgg ccctgccctc caaatatgtg aacttcctgt acctggccag 3781 ccactatgag aagctgaagg gctcccccga ggataatgag cagaaacagc tgtttgtgga 3841 acagcacaag cactacctgg acgagatcat cgagcagatc agcgagttct ccaagagagt 3901 gatcctggcc gacgctaatc tggacaaagt gctgtccgcc tacaacaagc accgggataa 3961 gcccatcaga gagcaggccg agaatatcat ccacctgttt accctgacca atctgggagc 4021 ccctgccgcc ttcaagtact ttgacaccac catcgaccgg aagaggtaca ccagcaccaa 4081 agaggtgctg gacgccaccc tgatccacca gagcatcacc ggcctgtacg agacacggat 4141 cgacctgtct cagctgggag gcgactacta gaggcaggct cc //