##gff-version 3
##sequence-region BBa_K1150063 1 4182
BBa_K1150063	.	tag	1	27	.	+	0	ID=BBa_K1150016;Name=BBa_K1150016
BBa_K1150063	.	tag	1	27	.	+	0	ID=annotation2347937;Name=HA;Parent=BBa_K1150016
BBa_K1150063	.	CDS	65	4165	.	+	0	ID=BBa_K1150000;Name=BBa_K1150000
BBa_K1150063	.	CDS	65	4165	.	+	0	ID=annotation2347618;Name=dCas9;Parent=BBa_K1150000
BBa_K1150063	.	sequence_alteration	2579	2581	.	+	0	ID=annotation2354601;Name=Nickaseless mutation;Parent=BBa_K1150000
BBa_K1150063	.	CDS	4174	4182	.	+	0	ID=BBa_K1150013;Name=BBa_K1150013
BBa_K1150063	.	sequence_feature	4174	4182	.	+	0	ID=annotation2347934;Name=AGS Linker;Parent=BBa_K1150013
BBa_K1150063	.	engineered_region	36	56	.	+	0	ID=BBa_K1150010;Name=BBa_K1150010
BBa_K1150063	.	CDS	36	56	.	+	0	ID=annotation2347465;Name=NLS;Parent=BBa_K1150010
>BBa_K1150063
tacccatacgatgttccagattacgcttactagagcctgcaagaaaaagattgaattactagaggacaagaagtacagc
atcggcctggccatcggcaccaactctgtgggctgggccgtgatcaccgacgagtacaaggtgcccagcaagaaattca
aggtgctgggcaacaccgaccggcacagcatcaagaagaacctgatcggagccctgctgttcgacagcggcgaaacagc
cgaggccacccggctgaagagaaccgccagaagaagatacaccagacggaagaaccggatctgctatctgcaagagatc
ttcagcaacgagatggccaaggtggacgacagcttcttccacagactggaagagtccttcctggtggaagaggataaga
agcacgagcggcaccccatcttcggcaacatcgtggacgaggtggcctaccacgagaagtaccccaccatctaccacct
gagaaagaaactggtggacagcaccgacaaggccgacctgcggctgatctatctggccctggcccacatgatcaagttc
cggggccacttcctgatcgagggcgacctgaaccccgacaacagcgacgtggacaagctgttcatccagctggtgcaga
cctacaaccagctgttcgaggaaaaccccatcaacgccagcggcgtggacgccaaggccatcctgtctgccagactgag
caagagcagacggctggaaaatctgatcgcccagctgcccggcgagaagaagaatggcctgttcggcaacctgattgcc
ctgagcctgggcctgacccccaacttcaagagcaacttcgacctggccgaggatgccaaacttcagctgagcaaggaca
cctacgacgacgacctggacaacctgctggcccagatcggcgaccagtacgccgacctgtttctggccgccaagaacct
gtccgacgccatcctgctgagcgacatcctgagagtgaacaccgagatcaccaaggcccccctgagcgcctctatgatc
aagagatacgacgagcaccaccaggacctgaccctgctgaaagctctcgtgcggcagcagctgcctgagaagtacaaag
agattttcttcgaccagagcaagaacggctacgccggatacattgacggcggagccagccaggaagagttctacaagtt
catcaagcccatcctggaaaagatggacggcaccgaggaactgctcgtgaagctgaacagagaggacctgctgcggaag
cagcggaccttcgacaacggcagcatcccccaccagatccacctgggagagctgcacgccattctgcggcggcaggaag
atttttacccattcctgaaggacaaccgggaaaagatcgagaagatcctgaccttccgcatcccctactacgtgggccc
tctggccaggggaaacagcagattcgcctggatgaccagaaagagcgaggaaaccatcaccccctggaacttcgaggaa
gtggtggacaagggcgcttccgcccagagcttcatcgagcggatgaccaacttcgataagaacctgcccaacgagaagg
tgctgcccaagcacagcctgctgtacgagtacttcaccgtgtataacgagctgaccaaagtgaaatacgtgaccgaggg
aatgagaaagcccgccttcctgagcggcgagcagaaaaaggccatcgtggacctgctgttcaagaccaaccggaaagtg
accgtgaagcagctgaaagaggactacttcaagaaaatcgagtgcttcgactccgtggaaatctccggcgtggaagatc
ggttcaacgcctccctgggcacataccacgatctgctgaaaattatcaaggacaaggacttcctggacaatgaggaaaa
cgaggacattctggaagatatcgtgctgaccctgacactgtttgaggacagagagatgatcgaggaacggctgaaaacc
tatgcccacctgttcgacgacaaagtgatgaagcagctgaagcggcggagatacaccggctggggcaggctgagccgga
agctgatcaacggcatccgggacaagcagtccggcaagacaatcctggatttcctgaagtccgacggcttcgccaacag
aaacttcatgcagctgatccacgacgacagcctgacctttaaagaggacatccagaaagcccaggtgtccggccagggc
gatagcctgcacgagcacattgccaatctggcaggcagccccgccattaagaagggcatcctgcaaacagtgaaggtgg
tggacgagctcgtgaaagtgatgggacggcacaagcccgagaacatcgtgatcgaaatggccagagagaaccagaccac
ccagaagggacagaagaacagccgcgagagaatgaagcggatcgaagagggcatcaaagagctgggcagccagatcctg
aaagaacaccccgtggaaaacacccagcttcagaacgagaagctgtacctgtactacctgcaaaatgggcgggatatgt
acgtggaccaggaactggacatcaaccggctgtccgactacgatgtggacgctatcgtgcctcagagctttctgaagga
cgactccatcgacaacaaggtgctgaccagaagcgacaagaaccggggcaagagcgacaacgtgccctccgaagaggtc
gtgaagaagatgaagaactactggcggcagctgctgaacgccaagctgattacccagagaaagttcgacaatctgacca
aggccgagagaggcggcctgagcgaactggataaggccggattcatcaagagacagctggtggaaacccggcagatcac
aaagcacgtggcacagatcctggactcccggatgaacactaagtacgacgagaatgacaagctgatccgggaagtgaaa
gtgatcaccctgaagtccaagctggtgtccgatttccggaaggatttccagttttacaaagtgcgcgagatcaacaact
accaccacgcccacgacgcctacctgaacgccgtcgtgggaaccgccctgatcaaaaagtaccctaagctggaaagcga
gttcgtgtacggcgactacaaggtgtacgacgtgcggaagatgatcgccaagagcgagcaggaaatcggcaaggctacc
gccaagtacttcttctacagcaacatcatgaactttttcaagaccgagattaccctggccaacggcgagatccggaagc
ggcctctgatcgagacaaacggcgaaaccggggagatcgtgtgggataagggccgggattttgccaccgtgcggaaagt
gctgagcatgccccaagtgaatatcgtgaaaaagaccgaggtgcagacaggcggcttcagcaaagagtctatcctgccc
aagaggaacagcgataagctgatcgccagaaagaaggactgggaccctaagaagtacggcggcttcgacagccccaccg
tggcctattctgtgctggtggtggccaaagtggaaaagggcaagtccaagaaactgaagagtgtgaaagagctgctggg
gatcaccatcatggaaagaagcagcttcgagaagaatcccatcgactttctggaagccaagggctacaaagaagtgaaa
aaggacctgatcatcaagctgcctaagtactccctgttcgagctggaaaacggccggaagagaatgctggcctctgcgg
gcgaactgcaaaagggaaacgaactggccctgccctccaaatatgtgaacttcctgtacctggccagccactatgagaa
gctgaagggctcccccgaggataatgagcagaaacagctgtttgtggaacagcacaagcactacctggacgagatcatc
gagcagatcagcgagttctccaagagagtgatcctggccgacgctaatctggacaaagtgctgtccgcctacaacaagc
accgggataagcccatcagagagcaggccgagaatatcatccacctgtttaccctgaccaatctgggagcccctgccgc
cttcaagtactttgacaccaccatcgaccggaagaggtacaccagcaccaaagaggtgctggacgccaccctgatccac
cagagcatcaccggcctgtacgagacacggatcgacctgtctcagctgggaggcgactactagaggcaggctcc