LOCUS BBa_K115012 1248 bp DNA linear 22-Dec-2024 DEFINITION Standard Luciferase Expression ACCESSION BBa_K115012 VERSION BBa_K115012.1 FEATURES Location/Qualifiers misc_feature 4..29 /label=c-amp2 misc_feature 6..44 /label=araO1 misc_feature 43..72 /label=c-amp1 misc_feature 73..101 /label=ara1 and ara2 promoter 113..118 /label=-35 promoter 136..141 /label=-10 promoter 1..149 /label=BBa_R0080 misc_feature 162..165 /label=conserved RBS 158..169 /label=BBa_B0034 misc_feature 176..1111 /label=Rluc CDS 176..1111 /label=Rluc misc_feature 176..1111 /label=BBa_J52008 stem_loop 1131..1174 /label=stem_loop misc_feature 1120..1199 /label=BBa_B0010 terminator 1120..1199 /label=BBa_B0010 stem_loop 1215..1234 /label=T7 TE misc_feature 1241..1241 /label=stop polyA_site 1235..1248 /label=polya misc_feature 1208..1248 /label=BBa_B0012 terminator 1208..1248 /label=BBa_B0012 ORIGIN 1 gcgtaacaaa agtgtctata atcacggcag aaaagtccac attgattatt tgcacggcgt 61 cacactttgc tatgccatag catttttatc cataagatta gcggatccta cctgacgctt 121 tttatcgcaa ctctctactg tttctccatt actagagaaa gaggagaaat actagatggc 181 ttccaaggtg tacgaccccg agcaacgcaa acgcatgatc actgggcctc agtggtgggc 241 tcgctgcaag caaatgaacg tgctggactc cttcatcaac tactatgatt ccgagaagca 301 cgccgagaac gccgtgattt ttctgcatgg taacgctgcc tccagctacc tgtggaggca 361 cgtcgtgcct cacatcgagc ccgtggctag atgcatcatc cctgatctga tcggaatggg 421 taagtccggc aagagcggga atggctcata tcgcctcctg gatcactaca agtacctcac 481 cgcttggttc gagctgctga accttccaaa gaaaatcatc tttgtgggcc acgactgggg 541 ggcttgtctg gcctttcact actcctacga gcaccaagac aagatcaagg ccatcgtcca 601 tgctgagagt gtcgtggacg tgatcgagtc ctgggacgag tggcctgaca tcgaggagga 661 tatcgccctg atcaagagcg aagagggcga gaaaatggtg cttgagaata acttcttcgt 721 cgagaccatg ctcccaagca agatcatgcg gaaactggag cctgaggagt tcgctgccta 781 cctggagcca ttcaaggaga agggcgaggt tagacggcct accctctcct ggcctcgcga 841 gatccctctc gttaagggag gcaagcccga cgtcgtccag attgtccgca actacaacgc 901 ctaccttcgg gccagcgacg atctgcctaa gatgttcatc gagtccgacc ctgggttctt 961 ttccaacgct attgtcgagg gagctaagaa gttccctaac accgagttcg tgaaggtgaa 1021 gggcctccac ttcagccagg aggacgctcc agatgaaatg ggtaagtaca tcaagagctt 1081 cgtggagcgc gtgctgaaga acgagcagta atactagagc caggcatcaa ataaaacgaa 1141 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct 1201 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttata //