LOCUS       BBa_K115013             1383 bp    DNA     linear       22-Dec-2024
DEFINITION  Thermosensitive experimental expression
ACCESSION   BBa_K115013
VERSION     BBa_K115013.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     stem_loop       209..223
                     /label=Predicted stem loop
     stem_loop       223..244
                     /label=Predicted stem loop
     stem_loop       246..279
                     /label=Predicted stem loop
     stem_loop       279..304
                     /label=Predicted stem loop extending to start codon
     RBS             297..303
                     /label=SD
     misc_feature    282..288
                     /label=Scar adaptation
     misc_feature    209..304
                     /label=BBa_K115008
     misc_feature    311..1246
                     /label=Rluc
     CDS             311..1246
                     /label=Rluc
     misc_feature    311..1246
                     /label=BBa_J52008
     stem_loop       1266..1309
                     /label=stem_loop
     misc_feature    1255..1334
                     /label=BBa_B0010
     terminator      1255..1334
                     /label=BBa_B0010
     stem_loop       1350..1369
                     /label=T7 TE
     misc_feature    1376..1376
                     /label=stop
     polyA_site      1370..1383
                     /label=polya
     misc_feature    1343..1383
                     /label=BBa_B0012
     terminator      1343..1383
                     /label=BBa_B0012
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagaggc cgcgacaagc ggtccgggcg ccctaggggc
      241 ccggcggaga cgggcgccgg aggtgtccga cgcctgctcg tcaagtactt gctccttgga
      301 ggattactag atggcttcca aggtgtacga ccccgagcaa cgcaaacgca tgatcactgg
      361 gcctcagtgg tgggctcgct gcaagcaaat gaacgtgctg gactccttca tcaactacta
      421 tgattccgag aagcacgccg agaacgccgt gatttttctg catggtaacg ctgcctccag
      481 ctacctgtgg aggcacgtcg tgcctcacat cgagcccgtg gctagatgca tcatccctga
      541 tctgatcgga atgggtaagt ccggcaagag cgggaatggc tcatatcgcc tcctggatca
      601 ctacaagtac ctcaccgctt ggttcgagct gctgaacctt ccaaagaaaa tcatctttgt
      661 gggccacgac tggggggctt gtctggcctt tcactactcc tacgagcacc aagacaagat
      721 caaggccatc gtccatgctg agagtgtcgt ggacgtgatc gagtcctggg acgagtggcc
      781 tgacatcgag gaggatatcg ccctgatcaa gagcgaagag ggcgagaaaa tggtgcttga
      841 gaataacttc ttcgtcgaga ccatgctccc aagcaagatc atgcggaaac tggagcctga
      901 ggagttcgct gcctacctgg agccattcaa ggagaagggc gaggttagac ggcctaccct
      961 ctcctggcct cgcgagatcc ctctcgttaa gggaggcaag cccgacgtcg tccagattgt
     1021 ccgcaactac aacgcctacc ttcgggccag cgacgatctg cctaagatgt tcatcgagtc
     1081 cgaccctggg ttcttttcca acgctattgt cgagggagct aagaagttcc ctaacaccga
     1141 gttcgtgaag gtgaagggcc tccacttcag ccaggaggac gctccagatg aaatgggtaa
     1201 gtacatcaag agcttcgtgg agcgcgtgct gaagaacgag cagtaatact agagccaggc
     1261 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
     1321 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt
     1381 ata
//