LOCUS       BBa_K115021             1175 bp    DNA     linear       25-Nov-2024
DEFINITION  construction of K115001 expression
ACCESSION   BBa_K115021
VERSION     BBa_K115021.1  
FEATURES             Location/Qualifiers
     RBS             90..95
                     /label=SD
     misc_feature    74..79
                     /label=Biobrick-Scar-adaptation
     stem_loop       1..15
                     /label=Predicted Stem Loop
     stem_loop       16..36
                     /label=Predicted Stem Loop
     stem_loop       39..71
                     /label=Predicted Stem Loop
     stem_loop       72..96
                     /label=Predicted Stem Loop extending to startcodon
     misc_feature    1..96
                     /label=BBa_K115001
     misc_feature    103..1038
                     /label=Rluc
     CDS             103..1038
                     /label=Rluc
     misc_feature    103..1038
                     /label=BBa_J52008
     stem_loop       1058..1101
                     /label=stem_loop
     misc_feature    1047..1126
                     /label=BBa_B0010
     terminator      1047..1126
                     /label=BBa_B0010
     stem_loop       1142..1161
                     /label=T7 TE
     misc_feature    1168..1168
                     /label=stop
     polyA_site      1162..1175
                     /label=polya
     misc_feature    1135..1175
                     /label=BBa_B0012
     terminator      1135..1175
                     /label=BBa_B0012
ORIGIN
        1 gccgcgacaa gcggtccggg cgccctaggg gcccggcgga gacgggcgcc ggaggtgtcc
       61 gacgcctgct cgtcaagttc ttgctccttg gaggattact agatggcttc caaggtgtac
      121 gaccccgagc aacgcaaacg catgatcact gggcctcagt ggtgggctcg ctgcaagcaa
      181 atgaacgtgc tggactcctt catcaactac tatgattccg agaagcacgc cgagaacgcc
      241 gtgatttttc tgcatggtaa cgctgcctcc agctacctgt ggaggcacgt cgtgcctcac
      301 atcgagcccg tggctagatg catcatccct gatctgatcg gaatgggtaa gtccggcaag
      361 agcgggaatg gctcatatcg cctcctggat cactacaagt acctcaccgc ttggttcgag
      421 ctgctgaacc ttccaaagaa aatcatcttt gtgggccacg actggggggc ttgtctggcc
      481 tttcactact cctacgagca ccaagacaag atcaaggcca tcgtccatgc tgagagtgtc
      541 gtggacgtga tcgagtcctg ggacgagtgg cctgacatcg aggaggatat cgccctgatc
      601 aagagcgaag agggcgagaa aatggtgctt gagaataact tcttcgtcga gaccatgctc
      661 ccaagcaaga tcatgcggaa actggagcct gaggagttcg ctgcctacct ggagccattc
      721 aaggagaagg gcgaggttag acggcctacc ctctcctggc ctcgcgagat ccctctcgtt
      781 aagggaggca agcccgacgt cgtccagatt gtccgcaact acaacgccta ccttcgggcc
      841 agcgacgatc tgcctaagat gttcatcgag tccgaccctg ggttcttttc caacgctatt
      901 gtcgagggag ctaagaagtt ccctaacacc gagttcgtga aggtgaaggg cctccacttc
      961 agccaggagg acgctccaga tgaaatgggt aagtacatca agagcttcgt ggagcgcgtg
     1021 ctgaagaacg agcagtaata ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa
     1081 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac
     1141 tggctcacct tcgggtgggc ctttctgcgt ttata
//