LOCUS       BBa_K115022             1131 bp    DNA     linear       22-Dec-2024
DEFINITION  construction of K115002 expression
ACCESSION   BBa_K115022
VERSION     BBa_K115022.1  
FEATURES             Location/Qualifiers
     RBS             47..52
                     /label=rbs
     misc_feature    23..28
                     /label=scar adaptation
     stem_loop       1..18
                     /label=stem_loop
     stem_loop       24..52
                     /label=predicted stem-loop extending to one position 
                     before start codon
     misc_feature    1..52
                     /label=BBa_K115002
     misc_feature    59..994
                     /label=Rluc
     CDS             59..994
                     /label=Rluc
     misc_feature    59..994
                     /label=BBa_J52008
     stem_loop       1014..1057
                     /label=stem_loop
     misc_feature    1003..1082
                     /label=BBa_B0010
     terminator      1003..1082
                     /label=BBa_B0010
     stem_loop       1098..1117
                     /label=T7 TE
     misc_feature    1124..1124
                     /label=stop
     polyA_site      1118..1131
                     /label=polya
     misc_feature    1091..1131
                     /label=BBa_B0012
     terminator      1091..1131
                     /label=BBa_B0012
ORIGIN
        1 ggacaagcaa tgcttgcctt gaatagtaac ttttgaatag tgattcagga ggtactagat
       61 ggcttccaag gtgtacgacc ccgagcaacg caaacgcatg atcactgggc ctcagtggtg
      121 ggctcgctgc aagcaaatga acgtgctgga ctccttcatc aactactatg attccgagaa
      181 gcacgccgag aacgccgtga tttttctgca tggtaacgct gcctccagct acctgtggag
      241 gcacgtcgtg cctcacatcg agcccgtggc tagatgcatc atccctgatc tgatcggaat
      301 gggtaagtcc ggcaagagcg ggaatggctc atatcgcctc ctggatcact acaagtacct
      361 caccgcttgg ttcgagctgc tgaaccttcc aaagaaaatc atctttgtgg gccacgactg
      421 gggggcttgt ctggcctttc actactccta cgagcaccaa gacaagatca aggccatcgt
      481 ccatgctgag agtgtcgtgg acgtgatcga gtcctgggac gagtggcctg acatcgagga
      541 ggatatcgcc ctgatcaaga gcgaagaggg cgagaaaatg gtgcttgaga ataacttctt
      601 cgtcgagacc atgctcccaa gcaagatcat gcggaaactg gagcctgagg agttcgctgc
      661 ctacctggag ccattcaagg agaagggcga ggttagacgg cctaccctct cctggcctcg
      721 cgagatccct ctcgttaagg gaggcaagcc cgacgtcgtc cagattgtcc gcaactacaa
      781 cgcctacctt cgggccagcg acgatctgcc taagatgttc atcgagtccg accctgggtt
      841 cttttccaac gctattgtcg agggagctaa gaagttccct aacaccgagt tcgtgaaggt
      901 gaagggcctc cacttcagcc aggaggacgc tccagatgaa atgggtaagt acatcaagag
      961 cttcgtggag cgcgtgctga agaacgagca gtaatactag agccaggcat caaataaaac
     1021 gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc
     1081 tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat a
//