LOCUS       BBa_K115028             1109 bp    DNA     linear       15-May-2024
DEFINITION  construction of K115020 expression
ACCESSION   BBa_K115028
VERSION     BBa_K115028.1  
FEATURES             Location/Qualifiers
     RBS             24..29
                     /label=SD
     misc_feature    1..30
                     /label=BBa_K115020
     misc_feature    37..972
                     /label=Rluc
     CDS             37..972
                     /label=Rluc
     misc_feature    37..972
                     /label=BBa_J52008
     stem_loop       992..1035
                     /label=stem_loop
     misc_feature    981..1060
                     /label=BBa_B0010
     terminator      981..1060
                     /label=BBa_B0010
     stem_loop       1076..1095
                     /label=T7 TE
     misc_feature    1102..1102
                     /label=stop
     polyA_site      1096..1109
                     /label=polya
     misc_feature    1069..1109
                     /label=BBa_B0012
     terminator      1069..1109
                     /label=BBa_B0012
     misc_feature    37..1109
                     /label=BBa_K115011
ORIGIN
        1 tgctcgtcaa gttcttgctc cttggaggat tactagatgg cttccaaggt gtacgacccc
       61 gagcaacgca aacgcatgat cactgggcct cagtggtggg ctcgctgcaa gcaaatgaac
      121 gtgctggact ccttcatcaa ctactatgat tccgagaagc acgccgagaa cgccgtgatt
      181 tttctgcatg gtaacgctgc ctccagctac ctgtggaggc acgtcgtgcc tcacatcgag
      241 cccgtggcta gatgcatcat ccctgatctg atcggaatgg gtaagtccgg caagagcggg
      301 aatggctcat atcgcctcct ggatcactac aagtacctca ccgcttggtt cgagctgctg
      361 aaccttccaa agaaaatcat ctttgtgggc cacgactggg gggcttgtct ggcctttcac
      421 tactcctacg agcaccaaga caagatcaag gccatcgtcc atgctgagag tgtcgtggac
      481 gtgatcgagt cctgggacga gtggcctgac atcgaggagg atatcgccct gatcaagagc
      541 gaagagggcg agaaaatggt gcttgagaat aacttcttcg tcgagaccat gctcccaagc
      601 aagatcatgc ggaaactgga gcctgaggag ttcgctgcct acctggagcc attcaaggag
      661 aagggcgagg ttagacggcc taccctctcc tggcctcgcg agatccctct cgttaaggga
      721 ggcaagcccg acgtcgtcca gattgtccgc aactacaacg cctaccttcg ggccagcgac
      781 gatctgccta agatgttcat cgagtccgac cctgggttct tttccaacgc tattgtcgag
      841 ggagctaaga agttccctaa caccgagttc gtgaaggtga agggcctcca cttcagccag
      901 gaggacgctc cagatgaaat gggtaagtac atcaagagct tcgtggagcg cgtgctgaag
      961 aacgagcagt aatactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg
     1021 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc
     1081 accttcgggt gggcctttct gcgtttata
//