LOCUS BBa_K115028 1109 bp DNA linear 22-Dec-2024 DEFINITION construction of K115020 expression ACCESSION BBa_K115028 VERSION BBa_K115028.1 FEATURES Location/Qualifiers RBS 24..29 /label=SD misc_feature 1..30 /label=BBa_K115020 misc_feature 37..972 /label=Rluc CDS 37..972 /label=Rluc misc_feature 37..972 /label=BBa_J52008 stem_loop 992..1035 /label=stem_loop misc_feature 981..1060 /label=BBa_B0010 terminator 981..1060 /label=BBa_B0010 stem_loop 1076..1095 /label=T7 TE misc_feature 1102..1102 /label=stop polyA_site 1096..1109 /label=polya misc_feature 1069..1109 /label=BBa_B0012 terminator 1069..1109 /label=BBa_B0012 misc_feature 37..1109 /label=BBa_K115011 ORIGIN 1 tgctcgtcaa gttcttgctc cttggaggat tactagatgg cttccaaggt gtacgacccc 61 gagcaacgca aacgcatgat cactgggcct cagtggtggg ctcgctgcaa gcaaatgaac 121 gtgctggact ccttcatcaa ctactatgat tccgagaagc acgccgagaa cgccgtgatt 181 tttctgcatg gtaacgctgc ctccagctac ctgtggaggc acgtcgtgcc tcacatcgag 241 cccgtggcta gatgcatcat ccctgatctg atcggaatgg gtaagtccgg caagagcggg 301 aatggctcat atcgcctcct ggatcactac aagtacctca ccgcttggtt cgagctgctg 361 aaccttccaa agaaaatcat ctttgtgggc cacgactggg gggcttgtct ggcctttcac 421 tactcctacg agcaccaaga caagatcaag gccatcgtcc atgctgagag tgtcgtggac 481 gtgatcgagt cctgggacga gtggcctgac atcgaggagg atatcgccct gatcaagagc 541 gaagagggcg agaaaatggt gcttgagaat aacttcttcg tcgagaccat gctcccaagc 601 aagatcatgc ggaaactgga gcctgaggag ttcgctgcct acctggagcc attcaaggag 661 aagggcgagg ttagacggcc taccctctcc tggcctcgcg agatccctct cgttaaggga 721 ggcaagcccg acgtcgtcca gattgtccgc aactacaacg cctaccttcg ggccagcgac 781 gatctgccta agatgttcat cgagtccgac cctgggttct tttccaacgc tattgtcgag 841 ggagctaaga agttccctaa caccgagttc gtgaaggtga agggcctcca cttcagccag 901 gaggacgctc cagatgaaat gggtaagtac atcaagagct tcgtggagcg cgtgctgaag 961 aacgagcagt aatactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg 1021 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc 1081 accttcgggt gggcctttct gcgtttata //