LOCUS       BBa_K115030             1288 bp    DNA     linear       22-Dec-2024
DEFINITION  Expression of luciferase with RNA-thermometer K115002
ACCESSION   BBa_K115030
VERSION     BBa_K115030.1  
FEATURES             Location/Qualifiers
     misc_feature    4..29
                     /label=c-amp2
     misc_feature    6..44
                     /label=araO1
     misc_feature    43..72
                     /label=c-amp1
     misc_feature    73..101
                     /label=ara1 and ara2
     promoter        113..118
                     /label=-35
     promoter        136..141
                     /label=-10
     promoter        1..149
                     /label=BBa_R0080
     RBS             204..209
                     /label=rbs
     misc_feature    180..185
                     /label=scar adaptation
     stem_loop       158..175
                     /label=stem_loop
     stem_loop       181..209
                     /label=predicted stem-loop extending to one position 
                     before start codon
     misc_feature    158..209
                     /label=BBa_K115002
     misc_feature    216..1151
                     /label=Rluc
     CDS             216..1151
                     /label=Rluc
     misc_feature    216..1151
                     /label=BBa_J52008
     stem_loop       1171..1214
                     /label=stem_loop
     misc_feature    1160..1239
                     /label=BBa_B0010
     terminator      1160..1239
                     /label=BBa_B0010
     stem_loop       1255..1274
                     /label=T7 TE
     misc_feature    1281..1281
                     /label=stop
     polyA_site      1275..1288
                     /label=polya
     misc_feature    1248..1288
                     /label=BBa_B0012
     terminator      1248..1288
                     /label=BBa_B0012
ORIGIN
        1 gcgtaacaaa agtgtctata atcacggcag aaaagtccac attgattatt tgcacggcgt
       61 cacactttgc tatgccatag catttttatc cataagatta gcggatccta cctgacgctt
      121 tttatcgcaa ctctctactg tttctccatt actagaggga caagcaatgc ttgccttgaa
      181 tagtaacttt tgaatagtga ttcaggaggt actagatggc ttccaaggtg tacgaccccg
      241 agcaacgcaa acgcatgatc actgggcctc agtggtgggc tcgctgcaag caaatgaacg
      301 tgctggactc cttcatcaac tactatgatt ccgagaagca cgccgagaac gccgtgattt
      361 ttctgcatgg taacgctgcc tccagctacc tgtggaggca cgtcgtgcct cacatcgagc
      421 ccgtggctag atgcatcatc cctgatctga tcggaatggg taagtccggc aagagcggga
      481 atggctcata tcgcctcctg gatcactaca agtacctcac cgcttggttc gagctgctga
      541 accttccaaa gaaaatcatc tttgtgggcc acgactgggg ggcttgtctg gcctttcact
      601 actcctacga gcaccaagac aagatcaagg ccatcgtcca tgctgagagt gtcgtggacg
      661 tgatcgagtc ctgggacgag tggcctgaca tcgaggagga tatcgccctg atcaagagcg
      721 aagagggcga gaaaatggtg cttgagaata acttcttcgt cgagaccatg ctcccaagca
      781 agatcatgcg gaaactggag cctgaggagt tcgctgccta cctggagcca ttcaaggaga
      841 agggcgaggt tagacggcct accctctcct ggcctcgcga gatccctctc gttaagggag
      901 gcaagcccga cgtcgtccag attgtccgca actacaacgc ctaccttcgg gccagcgacg
      961 atctgcctaa gatgttcatc gagtccgacc ctgggttctt ttccaacgct attgtcgagg
     1021 gagctaagaa gttccctaac accgagttcg tgaaggtgaa gggcctccac ttcagccagg
     1081 aggacgctcc agatgaaatg ggtaagtaca tcaagagctt cgtggagcgc gtgctgaaga
     1141 acgagcagta atactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg
     1201 gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca
     1261 ccttcgggtg ggcctttctg cgtttata
//