LOCUS       BBa_K115031             1345 bp    DNA     linear       14-May-2024
DEFINITION  Expression of luciferase with RNA-thermometer K115003
ACCESSION   BBa_K115031
VERSION     BBa_K115031.1  
FEATURES             Location/Qualifiers
     misc_feature    4..29
                     /label=c-amp2
     misc_feature    6..44
                     /label=araO1
     misc_feature    43..72
                     /label=c-amp1
     misc_feature    73..101
                     /label=ara1 and ara2
     promoter        113..118
                     /label=-35
     promoter        136..141
                     /label=-10
     promoter        1..149
                     /label=BBa_R0080
     RBS             263..266
                     /label=SD
     stem_loop       185..266
                     /label=Predicted stem loop up to A of AUG
     misc_feature    158..266
                     /label=BBa_K115003
     misc_feature    273..1208
                     /label=Rluc
     CDS             273..1208
                     /label=Rluc
     misc_feature    273..1208
                     /label=BBa_J52008
     stem_loop       1228..1271
                     /label=stem_loop
     misc_feature    1217..1296
                     /label=BBa_B0010
     terminator      1217..1296
                     /label=BBa_B0010
     stem_loop       1312..1331
                     /label=T7 TE
     misc_feature    1338..1338
                     /label=stop
     polyA_site      1332..1345
                     /label=polya
     misc_feature    1305..1345
                     /label=BBa_B0012
     terminator      1305..1345
                     /label=BBa_B0012
ORIGIN
        1 gcgtaacaaa agtgtctata atcacggcag aaaagtccac attgattatt tgcacggcgt
       61 cacactttgc tatgccatag catttttatc cataagatta gcggatccta cctgacgctt
      121 tttatcgcaa ctctctactg tttctccatt actagagtgt aaaaaatatt atttagcgtg
      181 actttctagt aacagctaac aattgttgtt actgcctaat gtttttaggg tattttaaaa
      241 aagggcgata aaaaacgatt gggggatact agatggcttc caaggtgtac gaccccgagc
      301 aacgcaaacg catgatcact gggcctcagt ggtgggctcg ctgcaagcaa atgaacgtgc
      361 tggactcctt catcaactac tatgattccg agaagcacgc cgagaacgcc gtgatttttc
      421 tgcatggtaa cgctgcctcc agctacctgt ggaggcacgt cgtgcctcac atcgagcccg
      481 tggctagatg catcatccct gatctgatcg gaatgggtaa gtccggcaag agcgggaatg
      541 gctcatatcg cctcctggat cactacaagt acctcaccgc ttggttcgag ctgctgaacc
      601 ttccaaagaa aatcatcttt gtgggccacg actggggggc ttgtctggcc tttcactact
      661 cctacgagca ccaagacaag atcaaggcca tcgtccatgc tgagagtgtc gtggacgtga
      721 tcgagtcctg ggacgagtgg cctgacatcg aggaggatat cgccctgatc aagagcgaag
      781 agggcgagaa aatggtgctt gagaataact tcttcgtcga gaccatgctc ccaagcaaga
      841 tcatgcggaa actggagcct gaggagttcg ctgcctacct ggagccattc aaggagaagg
      901 gcgaggttag acggcctacc ctctcctggc ctcgcgagat ccctctcgtt aagggaggca
      961 agcccgacgt cgtccagatt gtccgcaact acaacgccta ccttcgggcc agcgacgatc
     1021 tgcctaagat gttcatcgag tccgaccctg ggttcttttc caacgctatt gtcgagggag
     1081 ctaagaagtt ccctaacacc gagttcgtga aggtgaaggg cctccacttc agccaggagg
     1141 acgctccaga tgaaatgggt aagtacatca agagcttcgt ggagcgcgtg ctgaagaacg
     1201 agcagtaata ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc
     1261 tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct
     1321 tcgggtgggc ctttctgcgt ttata
//