LOCUS       BBa_K115032             1332 bp    DNA     linear       14-May-2024
DEFINITION  Expression of luciferase with RNA-thermometer K115008
ACCESSION   BBa_K115032
VERSION     BBa_K115032.1  
FEATURES             Location/Qualifiers
     misc_feature    4..29
                     /label=c-amp2
     misc_feature    6..44
                     /label=araO1
     misc_feature    43..72
                     /label=c-amp1
     misc_feature    73..101
                     /label=ara1 and ara2
     promoter        113..118
                     /label=-35
     promoter        136..141
                     /label=-10
     promoter        1..149
                     /label=BBa_R0080
     stem_loop       158..172
                     /label=Predicted stem loop
     stem_loop       172..193
                     /label=Predicted stem loop
     stem_loop       195..228
                     /label=Predicted stem loop
     stem_loop       228..253
                     /label=Predicted stem loop extending to start codon
     RBS             246..252
                     /label=SD
     misc_feature    231..237
                     /label=Scar adaptation
     misc_feature    158..253
                     /label=BBa_K115008
     misc_feature    260..1195
                     /label=Rluc
     CDS             260..1195
                     /label=Rluc
     misc_feature    260..1195
                     /label=BBa_J52008
     stem_loop       1215..1258
                     /label=stem_loop
     misc_feature    1204..1283
                     /label=BBa_B0010
     terminator      1204..1283
                     /label=BBa_B0010
     stem_loop       1299..1318
                     /label=T7 TE
     misc_feature    1325..1325
                     /label=stop
     polyA_site      1319..1332
                     /label=polya
     misc_feature    1292..1332
                     /label=BBa_B0012
     terminator      1292..1332
                     /label=BBa_B0012
ORIGIN
        1 gcgtaacaaa agtgtctata atcacggcag aaaagtccac attgattatt tgcacggcgt
       61 cacactttgc tatgccatag catttttatc cataagatta gcggatccta cctgacgctt
      121 tttatcgcaa ctctctactg tttctccatt actagaggcc gcgacaagcg gtccgggcgc
      181 cctaggggcc cggcggagac gggcgccgga ggtgtccgac gcctgctcgt caagtacttg
      241 ctccttggag gattactaga tggcttccaa ggtgtacgac cccgagcaac gcaaacgcat
      301 gatcactggg cctcagtggt gggctcgctg caagcaaatg aacgtgctgg actccttcat
      361 caactactat gattccgaga agcacgccga gaacgccgtg atttttctgc atggtaacgc
      421 tgcctccagc tacctgtgga ggcacgtcgt gcctcacatc gagcccgtgg ctagatgcat
      481 catccctgat ctgatcggaa tgggtaagtc cggcaagagc gggaatggct catatcgcct
      541 cctggatcac tacaagtacc tcaccgcttg gttcgagctg ctgaaccttc caaagaaaat
      601 catctttgtg ggccacgact ggggggcttg tctggccttt cactactcct acgagcacca
      661 agacaagatc aaggccatcg tccatgctga gagtgtcgtg gacgtgatcg agtcctggga
      721 cgagtggcct gacatcgagg aggatatcgc cctgatcaag agcgaagagg gcgagaaaat
      781 ggtgcttgag aataacttct tcgtcgagac catgctccca agcaagatca tgcggaaact
      841 ggagcctgag gagttcgctg cctacctgga gccattcaag gagaagggcg aggttagacg
      901 gcctaccctc tcctggcctc gcgagatccc tctcgttaag ggaggcaagc ccgacgtcgt
      961 ccagattgtc cgcaactaca acgcctacct tcgggccagc gacgatctgc ctaagatgtt
     1021 catcgagtcc gaccctgggt tcttttccaa cgctattgtc gagggagcta agaagttccc
     1081 taacaccgag ttcgtgaagg tgaagggcct ccacttcagc caggaggacg ctccagatga
     1141 aatgggtaag tacatcaaga gcttcgtgga gcgcgtgctg aagaacgagc agtaatacta
     1201 gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct
     1261 gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt
     1321 tctgcgttta ta
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