LOCUS       BBa_K115034             1319 bp    DNA     linear       14-May-2024
DEFINITION  Expression of luciferase with RNA-thermometer K115016
ACCESSION   BBa_K115034
VERSION     BBa_K115034.1  
FEATURES             Location/Qualifiers
     misc_feature    4..29
                     /label=c-amp2
     misc_feature    6..44
                     /label=araO1
     misc_feature    43..72
                     /label=c-amp1
     misc_feature    73..101
                     /label=ara1 and ara2
     promoter        113..118
                     /label=-35
     promoter        136..141
                     /label=-10
     promoter        1..149
                     /label=BBa_R0080
     RBS             234..239
                     /label=SD
     misc_feature    158..240
                     /label=BBa_K115016
     misc_feature    247..1182
                     /label=Rluc
     CDS             247..1182
                     /label=Rluc
     misc_feature    247..1182
                     /label=BBa_J52008
     stem_loop       1202..1245
                     /label=stem_loop
     misc_feature    1191..1270
                     /label=BBa_B0010
     terminator      1191..1270
                     /label=BBa_B0010
     stem_loop       1286..1305
                     /label=T7 TE
     misc_feature    1312..1312
                     /label=stop
     polyA_site      1306..1319
                     /label=polya
     misc_feature    1279..1319
                     /label=BBa_B0012
     terminator      1279..1319
                     /label=BBa_B0012
ORIGIN
        1 gcgtaacaaa agtgtctata atcacggcag aaaagtccac attgattatt tgcacggcgt
       61 cacactttgc tatgccatag catttttatc cataagatta gcggatccta cctgacgctt
      121 tttatcgcaa ctctctactg tttctccatt actagagccg ggcgcccttc gggggcccgg
      181 cggagacggg cgccggaggt gtccgacgcc tgattattca gtaaatcatc ttgggaggat
      241 tactagatgg cttccaaggt gtacgacccc gagcaacgca aacgcatgat cactgggcct
      301 cagtggtggg ctcgctgcaa gcaaatgaac gtgctggact ccttcatcaa ctactatgat
      361 tccgagaagc acgccgagaa cgccgtgatt tttctgcatg gtaacgctgc ctccagctac
      421 ctgtggaggc acgtcgtgcc tcacatcgag cccgtggcta gatgcatcat ccctgatctg
      481 atcggaatgg gtaagtccgg caagagcggg aatggctcat atcgcctcct ggatcactac
      541 aagtacctca ccgcttggtt cgagctgctg aaccttccaa agaaaatcat ctttgtgggc
      601 cacgactggg gggcttgtct ggcctttcac tactcctacg agcaccaaga caagatcaag
      661 gccatcgtcc atgctgagag tgtcgtggac gtgatcgagt cctgggacga gtggcctgac
      721 atcgaggagg atatcgccct gatcaagagc gaagagggcg agaaaatggt gcttgagaat
      781 aacttcttcg tcgagaccat gctcccaagc aagatcatgc ggaaactgga gcctgaggag
      841 ttcgctgcct acctggagcc attcaaggag aagggcgagg ttagacggcc taccctctcc
      901 tggcctcgcg agatccctct cgttaaggga ggcaagcccg acgtcgtcca gattgtccgc
      961 aactacaacg cctaccttcg ggccagcgac gatctgccta agatgttcat cgagtccgac
     1021 cctgggttct tttccaacgc tattgtcgag ggagctaaga agttccctaa caccgagttc
     1081 gtgaaggtga agggcctcca cttcagccag gaggacgctc cagatgaaat gggtaagtac
     1141 atcaagagct tcgtggagcg cgtgctgaag aacgagcagt aatactagag ccaggcatca
     1201 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt
     1261 gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct gcgtttata
//