LOCUS       BBa_K115036             1266 bp    DNA     linear       14-May-2024
DEFINITION  Expression of luciferase with RNA-thermometer K115020
ACCESSION   BBa_K115036
VERSION     BBa_K115036.1  
FEATURES             Location/Qualifiers
     misc_feature    4..29
                     /label=c-amp2
     misc_feature    6..44
                     /label=araO1
     misc_feature    43..72
                     /label=c-amp1
     misc_feature    73..101
                     /label=ara1 and ara2
     promoter        113..118
                     /label=-35
     promoter        136..141
                     /label=-10
     promoter        1..149
                     /label=BBa_R0080
     RBS             181..186
                     /label=SD
     misc_feature    158..187
                     /label=BBa_K115020
     misc_feature    194..1129
                     /label=Rluc
     CDS             194..1129
                     /label=Rluc
     misc_feature    194..1129
                     /label=BBa_J52008
     stem_loop       1149..1192
                     /label=stem_loop
     misc_feature    1138..1217
                     /label=BBa_B0010
     terminator      1138..1217
                     /label=BBa_B0010
     stem_loop       1233..1252
                     /label=T7 TE
     misc_feature    1259..1259
                     /label=stop
     polyA_site      1253..1266
                     /label=polya
     misc_feature    1226..1266
                     /label=BBa_B0012
     terminator      1226..1266
                     /label=BBa_B0012
     misc_feature    194..1266
                     /label=BBa_K115011
     misc_feature    158..1266
                     /label=BBa_K115028
ORIGIN
        1 gcgtaacaaa agtgtctata atcacggcag aaaagtccac attgattatt tgcacggcgt
       61 cacactttgc tatgccatag catttttatc cataagatta gcggatccta cctgacgctt
      121 tttatcgcaa ctctctactg tttctccatt actagagtgc tcgtcaagtt cttgctcctt
      181 ggaggattac tagatggctt ccaaggtgta cgaccccgag caacgcaaac gcatgatcac
      241 tgggcctcag tggtgggctc gctgcaagca aatgaacgtg ctggactcct tcatcaacta
      301 ctatgattcc gagaagcacg ccgagaacgc cgtgattttt ctgcatggta acgctgcctc
      361 cagctacctg tggaggcacg tcgtgcctca catcgagccc gtggctagat gcatcatccc
      421 tgatctgatc ggaatgggta agtccggcaa gagcgggaat ggctcatatc gcctcctgga
      481 tcactacaag tacctcaccg cttggttcga gctgctgaac cttccaaaga aaatcatctt
      541 tgtgggccac gactgggggg cttgtctggc ctttcactac tcctacgagc accaagacaa
      601 gatcaaggcc atcgtccatg ctgagagtgt cgtggacgtg atcgagtcct gggacgagtg
      661 gcctgacatc gaggaggata tcgccctgat caagagcgaa gagggcgaga aaatggtgct
      721 tgagaataac ttcttcgtcg agaccatgct cccaagcaag atcatgcgga aactggagcc
      781 tgaggagttc gctgcctacc tggagccatt caaggagaag ggcgaggtta gacggcctac
      841 cctctcctgg cctcgcgaga tccctctcgt taagggaggc aagcccgacg tcgtccagat
      901 tgtccgcaac tacaacgcct accttcgggc cagcgacgat ctgcctaaga tgttcatcga
      961 gtccgaccct gggttctttt ccaacgctat tgtcgaggga gctaagaagt tccctaacac
     1021 cgagttcgtg aaggtgaagg gcctccactt cagccaggag gacgctccag atgaaatggg
     1081 taagtacatc aagagcttcg tggagcgcgt gctgaagaac gagcagtaat actagagcca
     1141 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt
     1201 tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg
     1261 tttata
//