BBa_K115036
type: ComponentDefinition
persistent identity: BBa K115036
display id: BBa_K115036
version: 1
was generated by: iGEM to SBOL conversion
description: Expression of luciferase with RNA-thermometer K115020
created: 2008-09-03T11:00:00Z
modified: 2015-05-08T01:09:28Z
mutable provenance: See the subparts
owned by: james
owned by: myers
mutable description: Expression of luciferase with a ribosome binding site repressed by thermometer K115020. An arabinose inducible promoter is used.
discontinued: false
dominant: false
experience: experience/None
group u list: _223_
m user id: 0
owner id: 3007
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: see http://2008.igem.org/Team:TUDelft
star: true
creator: O.M.J.A. Stassen
type: DnaRegion
role: partType/Measurement
role: SO:0000804
component: BBa_K115028
component: BBa_R0080
sequence annotation: BBa_R0080
sequence annotation: BBa_K115028
sequence: https://synbiohub.org/public/igem/BBa_K115036_sequence/1

BBa_K115028
type: Component
persistent identity: component2222165
display id: component2222165
version: 1
top level: BBa_K115036
definition: BBa_K115028
access: public

BBa_R0080
type: Component
persistent identity: component2222152
display id: component2222152
version: 1
top level: BBa_K115036
definition: AraC
access: public

BBa_R0080
type: SequenceAnnotation
persistent identity: annotation2222152
display id: annotation2222152
version: 1
top level: BBa_K115036
location: https://synbiohub.org/public/igem/BBa_K115036/annotation2222152/range2222152/1
component: BBa_R0080

https://synbiohub.org/public/igem/BBa_K115036/annotation2222152/range2222152/1
type: Range
persistent identity: range2222152
display id: range2222152
version: 1
top level: BBa_K115036
direction: direction/reverse
start: 1
end: 149
orientation: inline

BBa_K115028
type: SequenceAnnotation
persistent identity: annotation2222165
display id: annotation2222165
version: 1
top level: BBa_K115036
location: https://synbiohub.org/public/igem/BBa_K115036/annotation2222165/range2222165/1
component: BBa_K115028

https://synbiohub.org/public/igem/BBa_K115036/annotation2222165/range2222165/1
type: Range
persistent identity: range2222165
display id: range2222165
version: 1
top level: BBa_K115036
direction: direction/reverse
start: 158
end: 1266
orientation: inline

AraC
type: ComponentDefinition
persistent identity: BBa R0080
display id: BBa_R0080
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (AraC regulated)
created: 2004-01-27T12:00:00Z
modified: 2015-05-08T01:14:15Z
mutable provenance: GenBank: J01641 (www.ncbi.nlm.nih.gov)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: AraC operator, truncated to include araO1, araI1, araI2, c-amp1, and c-amp2 sites. This operator should *activate* transcription in the presence of AraC; b/c the operator lacks the araO2 site, there should not be araC-mediated repression.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Sara Neves (Fighting Darwins)
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: c-amp2
sequence annotation: araO1
sequence annotation: c-amp1
sequence annotation: ara1 and ara2
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0080_sequence/1

c-amp2
type: SequenceAnnotation
persistent identity: annotation301456
display id: annotation301456
version: 1
top level: AraC
location: https://synbiohub.org/public/igem/BBa_R0080/annotation301456/range301456/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0080/annotation301456/range301456/1
type: Range
persistent identity: range301456
display id: range301456
version: 1
top level: AraC
direction: direction/forward
start: 4
end: 29
orientation: inline

araO1
type: SequenceAnnotation
persistent identity: annotation301457
display id: annotation301457
version: 1
top level: AraC
location: https://synbiohub.org/public/igem/BBa_R0080/annotation301457/range301457/1
role: feature/operator
role: SO:0000057

https://synbiohub.org/public/igem/BBa_R0080/annotation301457/range301457/1
type: Range
persistent identity: range301457
display id: range301457
version: 1
top level: AraC
direction: direction/forward
start: 6
end: 44
orientation: inline

c-amp1
type: SequenceAnnotation
persistent identity: annotation301458
display id: annotation301458
version: 1
top level: AraC
location: https://synbiohub.org/public/igem/BBa_R0080/annotation301458/range301458/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0080/annotation301458/range301458/1
type: Range
persistent identity: range301458
display id: range301458
version: 1
top level: AraC
direction: direction/forward
start: 43
end: 72
orientation: inline

ara1 and ara2
type: SequenceAnnotation
persistent identity: annotation301462
display id: annotation301462
version: 1
top level: AraC
location: https://synbiohub.org/public/igem/BBa_R0080/annotation301462/range301462/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0080/annotation301462/range301462/1
type: Range
persistent identity: range301462
display id: range301462
version: 1
top level: AraC
direction: direction/forward
start: 73
end: 101
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation308601
display id: annotation308601
version: 1
top level: AraC
location: https://synbiohub.org/public/igem/BBa_R0080/annotation308601/range308601/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0080/annotation308601/range308601/1
type: Range
persistent identity: range308601
display id: range308601
version: 1
top level: AraC
direction: direction/forward
start: 113
end: 118
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation308602
display id: annotation308602
version: 1
top level: AraC
location: https://synbiohub.org/public/igem/BBa_R0080/annotation308602/range308602/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0080/annotation308602/range308602/1
type: Range
persistent identity: range308602
display id: range308602
version: 1
top level: AraC
direction: direction/forward
start: 136
end: 141
orientation: inline

BBa_K115028
type: ComponentDefinition
persistent identity: BBa K115028
display id: BBa_K115028
version: 1
was generated by: iGEM to SBOL conversion
description: construction of K115020 expression
created: 2008-09-03T11:00:00Z
modified: 2015-05-08T01:09:28Z
mutable provenance: See the subparts
owned by: james
owned by: myers
mutable description: Construction Vector for interchangeable promoter
discontinued: false
dominant: false
experience: experience/None
group u list: _223_
m user id: 0
owner id: 3007
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: see http://2008.igem.org/Team:TUDelft
star: false
creator: O.M.J.A. Stassen
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_K115020
component: BBa_K115011
sequence annotation: BBa_K115020
sequence annotation: BBa_K115011
sequence: https://synbiohub.org/public/igem/BBa_K115028_sequence/1

BBa_K115020
type: Component
persistent identity: component2220421
display id: component2220421
version: 1
top level: BBa_K115028
definition: BBa_K115020
access: public

BBa_K115011
type: Component
persistent identity: component2220431
display id: component2220431
version: 1
top level: BBa_K115028
definition: BBa_K115011
access: public

BBa_K115020
type: SequenceAnnotation
persistent identity: annotation2220421
display id: annotation2220421
version: 1
top level: BBa_K115028
location: https://synbiohub.org/public/igem/BBa_K115028/annotation2220421/range2220421/1
component: BBa_K115020

https://synbiohub.org/public/igem/BBa_K115028/annotation2220421/range2220421/1
type: Range
persistent identity: range2220421
display id: range2220421
version: 1
top level: BBa_K115028
direction: direction/reverse
start: 1
end: 30
orientation: inline

BBa_K115011
type: SequenceAnnotation
persistent identity: annotation2220431
display id: annotation2220431
version: 1
top level: BBa_K115028
location: https://synbiohub.org/public/igem/BBa_K115028/annotation2220431/range2220431/1
component: BBa_K115011

https://synbiohub.org/public/igem/BBa_K115028/annotation2220431/range2220431/1
type: Range
persistent identity: range2220431
display id: range2220431
version: 1
top level: BBa_K115028
direction: direction/reverse
start: 37
end: 1109
orientation: inline

BBa_K115011
type: ComponentDefinition
persistent identity: BBa K115011
display id: BBa_K115011
version: 1
was generated by: iGEM to SBOL conversion
description: Luciferase, Terminator
created: 2008-08-17T11:00:00Z
modified: 2015-05-08T01:09:28Z
mutable provenance: Salmonella Enterica Tyhpy (CP000886.1) and Renilla Luciferase
owned by: james
owned by: myers
mutable description: This is a composite part to test the functionality of the RNA thermometer BBa_K115002 with the aid of Renilla luciferase.
discontinued: false
dominant: false
experience: experience/None
group u list: _223_
m user id: 0
owner id: 3007
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: none
star: false
creator: O.M.J.A. Stassen
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_J52008
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_J52008
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K115011_sequence/1

BBa_J52008
type: Component
persistent identity: component1974248
display id: component1974248
version: 1
top level: BBa_K115011
definition: BBa_J52008
access: public

BBa_B0012
type: Component
persistent identity: component1974251
display id: component1974251
version: 1
top level: BBa_K115011
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1974249
display id: component1974249
version: 1
top level: BBa_K115011
definition: BBa_B0010
access: public

BBa_J52008
type: SequenceAnnotation
persistent identity: annotation1974248
display id: annotation1974248
version: 1
top level: BBa_K115011
location: https://synbiohub.org/public/igem/BBa_K115011/annotation1974248/range1974248/1
component: BBa_J52008

https://synbiohub.org/public/igem/BBa_K115011/annotation1974248/range1974248/1
type: Range
persistent identity: range1974248
display id: range1974248
version: 1
top level: BBa_K115011
direction: direction/reverse
start: 1
end: 936
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1974249
display id: annotation1974249
version: 1
top level: BBa_K115011
location: https://synbiohub.org/public/igem/BBa_K115011/annotation1974249/range1974249/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K115011/annotation1974249/range1974249/1
type: Range
persistent identity: range1974249
display id: range1974249
version: 1
top level: BBa_K115011
direction: direction/forward
start: 945
end: 1024
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1974251
display id: annotation1974251
version: 1
top level: BBa_K115011
location: https://synbiohub.org/public/igem/BBa_K115011/annotation1974251/range1974251/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K115011/annotation1974251/range1974251/1
type: Range
persistent identity: range1974251
display id: range1974251
version: 1
top level: BBa_K115011
direction: direction/forward
start: 1033
end: 1073
orientation: inline

BBa_K115020
type: ComponentDefinition
persistent identity: BBa K115020
display id: BBa_K115020
version: 1
was generated by: iGEM to SBOL conversion
description: RNA thermometer (ROSE hairpin 37??C)
created: 2008-08-19T11:00:00Z
modified: 2015-05-08T01:09:28Z
mutable provenance: ROSE hairpin.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: An RNA thermometer that theoretically switches on translation at 37 degrees Celcius and switched of translation below that temperature. Still to be tested.
discontinued: false
dominant: false
experience: experience/None
group u list: _223_
m user id: 0
owner id: 3006
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: later.
star: true
creator: Bastiaan van den Berg
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence annotation: SD
sequence: https://synbiohub.org/public/igem/BBa_K115020_sequence/1

SD
type: SequenceAnnotation
persistent identity: annotation1972923
display id: annotation1972923
version: 1
top level: BBa_K115020
location: https://synbiohub.org/public/igem/BBa_K115020/annotation1972923/range1972923/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_K115020/annotation1972923/range1972923/1
type: Range
persistent identity: range1972923
display id: range1972923
version: 1
top level: BBa_K115020
direction: direction/forward
start: 24
end: 29
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

BBa_J52008
type: ComponentDefinition
persistent identity: BBa J52008
display id: BBa_J52008
version: 1
was generated by: iGEM to SBOL conversion
description: luciferase: luciferin 2-monooxygenase from Renilla reniformis (EC 1.13.12.5; SwissProt:: P27652)
created: 2006-10-13T11:00:00Z
modified: 2015-08-31T03:54:04Z
mutable provenance: -
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Rluc is a protein called Renilla`s luciferase and it emits light when adding the right substrate. That is why it can bi used as a reported to track other proteins or to monitor the activity of a promotor.
discontinued: false
dominant: true
experience: experience/Works
group u list: _80_
m user id: 0
owner id: 800
owning group id: 80
sample status: In stock
status: status/Available
bookmark: true
mutable notes: Rluc is cloned in BioBrick vector pSB1AK3.
star: true
creator: Monika Ciglic
type: DnaRegion
role: SO:0000804
role: partType/Reporter
sequence annotation: Rluc
sequence: https://synbiohub.org/public/igem/BBa_J52008_sequence/1

Rluc
type: SequenceAnnotation
persistent identity: annotation1902845
display id: annotation1902845
version: 1
top level: BBa_J52008
location: https://synbiohub.org/public/igem/BBa_J52008/annotation1902845/range1902845/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_J52008/annotation1902845/range1902845/1
type: Range
persistent identity: range1902845
display id: range1902845
version: 1
top level: BBa_J52008
direction: direction/forward
start: 1
end: 936
orientation: inline

Rluc
type: SequenceAnnotation
persistent identity: annotation1902833
display id: annotation1902833
version: 1
top level: BBa_J52008
location: https://synbiohub.org/public/igem/BBa_J52008/annotation1902833/range1902833/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_J52008/annotation1902833/range1902833/1
type: Range
persistent identity: range1902833
display id: range1902833
version: 1
top level: BBa_J52008
direction: direction/forward
start: 1
end: 936
orientation: inline

https://synbiohub.org/public/igem/BBa_K115036_sequence/1
type: Sequence
persistent identity: BBa K115036 sequence
display id: BBa_K115036_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccattactagagtgctcgtcaagttcttgctccttggaggattactagatggcttccaaggtgtacgaccccgagcaacgcaaacgcatgatcactgggcctcagtggtgggctcgctgcaagcaaatgaacgtgctggactccttcatcaactactatgattccgagaagcacgccgagaacgccgtgatttttctgcatggtaacgctgcctccagctacctgtggaggcacgtcgtgcctcacatcgagcccgtggctagatgcatcatccctgatctgatcggaatgggtaagtccggcaagagcgggaatggctcatatcgcctcctggatcactacaagtacctcaccgcttggttcgagctgctgaaccttccaaagaaaatcatctttgtgggccacgactggggggcttgtctggcctttcactactcctacgagcaccaagacaagatcaaggccatcgtccatgctgagagtgtcgtggacgtgatcgagtcctgggacgagtggcctgacatcgaggaggatatcgccctgatcaagagcgaagagggcgagaaaatggtgcttgagaataacttcttcgtcgagaccatgctcccaagcaagatcatgcggaaactggagcctgaggagttcgctgcctacctggagccattcaaggagaagggcgaggttagacggcctaccctctcctggcctcgcgagatccctctcgttaagggaggcaagcccgacgtcgtccagattgtccgcaactacaacgcctaccttcgggccagcgacgatctgcctaagatgttcatcgagtccgaccctgggttcttttccaacgctattgtcgagggagctaagaagttccctaacaccgagttcgtgaaggtgaagggcctccacttcagccaggaggacgctccagatgaaatgggtaagtacatcaagagcttcgtggagcgcgtgctgaagaacgagcagtaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0080_sequence/1
type: Sequence
persistent identity: BBa R0080 sequence
display id: BBa_R0080_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K115028_sequence/1
type: Sequence
persistent identity: BBa K115028 sequence
display id: BBa_K115028_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tgctcgtcaagttcttgctccttggaggattactagatggcttccaaggtgtacgaccccgagcaacgcaaacgcatgatcactgggcctcagtggtgggctcgctgcaagcaaatgaacgtgctggactccttcatcaactactatgattccgagaagcacgccgagaacgccgtgatttttctgcatggtaacgctgcctccagctacctgtggaggcacgtcgtgcctcacatcgagcccgtggctagatgcatcatccctgatctgatcggaatgggtaagtccggcaagagcgggaatggctcatatcgcctcctggatcactacaagtacctcaccgcttggttcgagctgctgaaccttccaaagaaaatcatctttgtgggccacgactggggggcttgtctggcctttcactactcctacgagcaccaagacaagatcaaggccatcgtccatgctgagagtgtcgtggacgtgatcgagtcctgggacgagtggcctgacatcgaggaggatatcgccctgatcaagagcgaagagggcgagaaaatggtgcttgagaataacttcttcgtcgagaccatgctcccaagcaagatcatgcggaaactggagcctgaggagttcgctgcctacctggagccattcaaggagaagggcgaggttagacggcctaccctctcctggcctcgcgagatccctctcgttaagggaggcaagcccgacgtcgtccagattgtccgcaactacaacgcctaccttcgggccagcgacgatctgcctaagatgttcatcgagtccgaccctgggttcttttccaacgctattgtcgagggagctaagaagttccctaacaccgagttcgtgaaggtgaagggcctccacttcagccaggaggacgctccagatgaaatgggtaagtacatcaagagcttcgtggagcgcgtgctgaagaacgagcagtaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K115011_sequence/1
type: Sequence
persistent identity: BBa K115011 sequence
display id: BBa_K115011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcttccaaggtgtacgaccccgagcaacgcaaacgcatgatcactgggcctcagtggtgggctcgctgcaagcaaatgaacgtgctggactccttcatcaactactatgattccgagaagcacgccgagaacgccgtgatttttctgcatggtaacgctgcctccagctacctgtggaggcacgtcgtgcctcacatcgagcccgtggctagatgcatcatccctgatctgatcggaatgggtaagtccggcaagagcgggaatggctcatatcgcctcctggatcactacaagtacctcaccgcttggttcgagctgctgaaccttccaaagaaaatcatctttgtgggccacgactggggggcttgtctggcctttcactactcctacgagcaccaagacaagatcaaggccatcgtccatgctgagagtgtcgtggacgtgatcgagtcctgggacgagtggcctgacatcgaggaggatatcgccctgatcaagagcgaagagggcgagaaaatggtgcttgagaataacttcttcgtcgagaccatgctcccaagcaagatcatgcggaaactggagcctgaggagttcgctgcctacctggagccattcaaggagaagggcgaggttagacggcctaccctctcctggcctcgcgagatccctctcgttaagggaggcaagcccgacgtcgtccagattgtccgcaactacaacgcctaccttcgggccagcgacgatctgcctaagatgttcatcgagtccgaccctgggttcttttccaacgctattgtcgagggagctaagaagttccctaacaccgagttcgtgaaggtgaagggcctccacttcagccaggaggacgctccagatgaaatgggtaagtacatcaagagcttcgtggagcgcgtgctgaagaacgagcagtaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K115020_sequence/1
type: Sequence
persistent identity: BBa K115020 sequence
display id: BBa_K115020_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tgctcgtcaagttcttgctccttggaggat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J52008_sequence/1
type: Sequence
persistent identity: BBa J52008 sequence
display id: BBa_J52008_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcttccaaggtgtacgaccccgagcaacgcaaacgcatgatcactgggcctcagtggtgggctcgctgcaagcaaatgaacgtgctggactccttcatcaactactatgattccgagaagcacgccgagaacgccgtgatttttctgcatggtaacgctgcctccagctacctgtggaggcacgtcgtgcctcacatcgagcccgtggctagatgcatcatccctgatctgatcggaatgggtaagtccggcaagagcgggaatggctcatatcgcctcctggatcactacaagtacctcaccgcttggttcgagctgctgaaccttccaaagaaaatcatctttgtgggccacgactggggggcttgtctggcctttcactactcctacgagcaccaagacaagatcaaggccatcgtccatgctgagagtgtcgtggacgtgatcgagtcctgggacgagtggcctgacatcgaggaggatatcgccctgatcaagagcgaagagggcgagaaaatggtgcttgagaataacttcttcgtcgagaccatgctcccaagcaagatcatgcggaaactggagcctgaggagttcgctgcctacctggagccattcaaggagaagggcgaggttagacggcctaccctctcctggcctcgcgagatccctctcgttaagggaggcaagcccgacgtcgtccagattgtccgcaactacaacgcctaccttcgggccagcgacgatctgcctaagatgttcatcgagtccgaccctgggttcttttccaacgctattgtcgagggagctaagaagttccctaacaccgagttcgtgaaggtgaagggcctccacttcagccaggaggacgctccagatgaaatgggtaagtacatcaagagcttcgtggagcgcgtgctgaagaacgagcagtaa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z