Expression of luciferase with RNA-thermometer K115020
Expression of luciferase with a ribosome binding site repressed by thermometer K115020. An arabinose inducible promoter is used.
AraC operator, truncated to include araO1, araI1, araI2, c-amp1, and c-amp2 sites. This operator should *activate* transcription in the presence of AraC; b/c the operator lacks the araO2 site, there should not be araC-mediated repression.
Construction Vector for interchangeable promoter
Salmonella Enterica Tyhpy (CP000886.1) and Renilla Luciferase
This is a composite part to test the functionality of the RNA thermometer BBa_K115002 with the aid of Renilla luciferase.
An RNA thermometer that theoretically switches on translation at 37 degrees Celcius and switched of translation below that temperature. Still to be tested.
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Transcription terminator for the E.coli RNA polymerase.
Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
Transcriptional terminator consisting of a 64 bp stem-loop.
luciferase: luciferin 2-monooxygenase from Renilla reniformis (EC 1.13.12.5; SwissProt:: P27652)
Rluc is a protein called Renilla`s luciferase and it emits light when adding the right substrate. That is why it can bi used as a reported to track other proteins or to monitor the activity of a promotor.
Rluc is cloned in BioBrick vector pSB1AK3.
Conversion of the iGEM parts registry to SBOL2.1