LOCUS       BBa_K1151006             810 bp    DNA     linear       22-Dec-2024
DEFINITION  HpNikR coding device
ACCESSION   BBa_K1151006
VERSION     BBa_K1151006.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    1..220
                     /label=BBa_J04500
     CDS             227..673
                     /label=BBa_K1151000
     stem_loop       693..736
                     /label=stem_loop
     misc_feature    682..761
                     /label=BBa_B0010
     terminator      682..761
                     /label=BBa_B0010
     stem_loop       777..796
                     /label=T7 TE
     misc_feature    803..803
                     /label=stop
     polyA_site      797..810
                     /label=polya
     misc_feature    770..810
                     /label=BBa_B0012
     terminator      770..810
                     /label=BBa_B0012
     terminator      682..810
                     /label=BBa_B0015
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgg atacacccaa
      241 taaagacgat tcaatcatcc gcttttcggt ttctttacaa caaaatttat tagacgaatt
      301 agacaaccgc atcattaaaa acggctattc ttctcgctca gaattagtgc gcgacatgat
      361 cagagaaaaa ttagtagaag acaattgggc cgaagataac cctaatgatg agagcaaaat
      421 cgccgtgctt gtggtgattt atgatcacca ccaaagggaa ttgaaccagc gcatgataga
      481 cattcagcat gccagcggga cgcatgtttt atgcaccact cacattcaca tggatgagca
      541 taattgttta gagacgatta ttttacaagg caattcgttt gaaatccaac gcttgcaact
      601 agaaattggg gggcttaggg gggttaaatt cgctaaattg actaaggcgt ctagctttga
      661 acacaatgaa tagtactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact
      721 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct
      781 caccttcggg tgggcctttc tgcgtttata
//