BBa_K1151008
1
BBa_K1151008
RFP nickel regulated
2013-09-22T11:00:00Z
2015-05-08T01:09:29Z
pUreA was synthesized starting from the genome sequence of H. pylori, instead the other parts coming from iGEM kit.
This part was built as a ligation product between the promoter of our design, pUreA, RBS (B0034), RFP (LVA tag) and the terminator B0015.
false
false
_1463_
0
16631
9
It's complicated
false
None
false
Gian Piero Lazzari
component2360860
1
BBa_K773003
component2360853
1
BBa_K1151005
annotation2360853
1
BBa_K1151005
range2360853
1
1
253
annotation2360860
1
BBa_K773003
range2360860
1
262
1026
BBa_K773003
1
BBa_K773003
mCherry-LVA
2012-09-30T11:00:00Z
2015-05-08T01:13:15Z
This part was modified from a plasmid provided by the Murray lab at Caltech.
Released HQ 2013
This part contains the fluorescent protein mCherry attached to a degradation tag. It will decrease the amount of fluorescent protein over time.
false
false
_1025_
0
11646
9
In stock
false
We modified the AAV sequence to LVA with PCR.
false
Katie Knister
annotation2209512
1
Stop
range2209512
1
760
762
annotation2209511
1
ssrA degradation tag: LVA
range2209511
1
730
759
annotation2209513
1
Stop
range2209513
1
763
765
annotation2209509
1
mCherry-LVA
range2209509
1
19
765
annotation2209510
1
Start
range2209510
1
19
21
annotation2209508
1
B0034
range2209508
1
1
12
BBa_K1151005
1
BBa_K1151005
pUreA
2013-09-20T11:00:00Z
2015-05-08T01:09:29Z
This part was synthesized by IDT.
This promoter derived from ureAB operon of Helicobacter pylori. It can be linked by HpNikR (BBa_K1151000) in nickel presence for promoting trascription of the downstream gene
false
false
_1463_
0
16632
9
It's complicated
false
None
false
Emanuele Rizzo
BBa_K1151008_sequence
1
ctcattctcctattcttaaagtgtttttccttgaagacataacgaaatcaaggttggatgtaattgtagcaatgttttgatttactaagattaacagaagcgttcattaactaattattatttaaaatgaattagtgttatatttttgaagcgctataaaagcgttattagaagcgcgtttaatacccctttagaatttataaagatcaaaaactcgcaaaaaaaatgagaattaaaattggagtgataatggtactagagaaagaggagaaatactagatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaaggcagcaaacgacgaaaactacgctctagtagcttaataa
BBa_K773003_sequence
1
aaagaggagaaatactagatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaaggcagcaaacgacgaaaactacgctctagtagcttaataa
BBa_K1151005_sequence
1
ctcattctcctattcttaaagtgtttttccttgaagacataacgaaatcaaggttggatgtaattgtagcaatgttttgatttactaagattaacagaagcgttcattaactaattattatttaaaatgaattagtgttatatttttgaagcgctataaaagcgttattagaagcgcgtttaatacccctttagaatttataaagatcaaaaactcgcaaaaaaaatgagaattaaaattggagtgataatgg
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z