LOCUS       BBa_K1151016            1877 bp    DNA     linear       14-Nov-2024
DEFINITION  LuxI-HpNikR
ACCESSION   BBa_K1151016
VERSION     BBa_K1151016.1  
FEATURES             Location/Qualifiers
     promoter        1..253
                     /label=BBa_K1151005
     misc_feature    266..269
                     /label=conserved
     RBS             262..273
                     /label=BBa_B0034
     misc_feature    859..890
                     /label=LVA
     CDS             280..858
                     /label=luxI
     misc_feature    280..897
                     /label=BBa_C0061
     misc_feature    898..922
                     /label=Help:Barcodes
     CDS             280..897
                     /label=BBa_C0061
     stem_loop       942..985
                     /label=stem_loop
     misc_feature    931..1010
                     /label=BBa_B0010
     terminator      931..1010
                     /label=BBa_B0010
     stem_loop       1026..1045
                     /label=T7 TE
     misc_feature    1052..1052
                     /label=stop
     polyA_site      1046..1059
                     /label=polya
     misc_feature    1019..1059
                     /label=BBa_B0012
     terminator      1019..1059
                     /label=BBa_B0012
     terminator      931..1059
                     /label=BBa_B0015
     misc_feature    262..1059
                     /label=BBa_K805016
     misc_feature    1..1059
                     /label=BBa_K1151012
     CDS             1068..1155
                     /label=end of LacI coding region (inactive)
     misc_feature    1068..1267
                     /label=BBa_R0010
     misc_feature    1156..1193
                     /label=CAP binding site
     promoter        1204..1209
                     /label=-35
     promoter        1228..1233
                     /label=-10
     misc_feature    1233..1267
                     /label=LacI binding site
     misc_feature    1240..1240
                     /label=start
     promoter        1068..1267
                     /label=BBa_R0010
     misc_feature    1280..1283
                     /label=conserved
     RBS             1276..1287
                     /label=BBa_B0034
     misc_feature    1068..1287
                     /label=BBa_J04500
     CDS             1294..1740
                     /label=BBa_K1151000
     stem_loop       1760..1803
                     /label=stem_loop
     misc_feature    1749..1828
                     /label=BBa_B0010
     terminator      1749..1828
                     /label=BBa_B0010
     stem_loop       1844..1863
                     /label=T7 TE
     misc_feature    1870..1870
                     /label=stop
     polyA_site      1864..1877
                     /label=polya
     misc_feature    1837..1877
                     /label=BBa_B0012
     terminator      1837..1877
                     /label=BBa_B0012
     terminator      1749..1877
                     /label=BBa_B0015
     misc_feature    1068..1877
                     /label=BBa_K1151006
ORIGIN
        1 ctcattctcc tattcttaaa gtgtttttcc ttgaagacat aacgaaatca aggttggatg
       61 taattgtagc aatgttttga tttactaaga ttaacagaag cgttcattaa ctaattatta
      121 tttaaaatga attagtgtta tatttttgaa gcgctataaa agcgttatta gaagcgcgtt
      181 taatacccct ttagaattta taaagatcaa aaactcgcaa aaaaaatgag aattaaaatt
      241 ggagtgataa tggtactaga gaaagaggag aaatactaga tgactataat gataaaaaaa
      301 tcggattttt tggcaattcc atcggaggag tataaaggta ttctaagtct tcgttatcaa
      361 gtgtttaagc aaagacttga gtgggactta gttgtagaaa ataaccttga atcagatgag
      421 tatgataact caaatgcaga atatatttat gcttgtgatg atactgaaaa tgtaagtgga
      481 tgctggcgtt tattacctac aacaggtgat tatatgctga aaagtgtttt tcctgaattg
      541 cttggtcaac agagtgctcc caaagatcct aatatagtcg aattaagtcg ttttgctgta
      601 ggtaaaaata gctcaaagat aaataactct gctagtgaaa ttacaatgaa actatttgaa
      661 gctatatata aacacgctgt tagtcaaggt attacagaat atgtaacagt aacatcaaca
      721 gcaatagagc gatttttaaa gcgtattaaa gttccttgtc atcgtattgg agacaaagaa
      781 attcatgtat taggtgatac taaatcggtt gtattgtcta tgcctattaa tgaacagttt
      841 aaaaaagcag tcttaaatgc tgcaaacgac gaaaactacg ctttagtagc ttaataactc
      901 tgatagtgct agtgtagatc tctactagag ccaggcatca aataaaacga aaggctcagt
      961 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc
     1021 acactggctc accttcgggt gggcctttct gcgtttatat actagagcaa tacgcaaacc
     1081 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
     1141 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
     1201 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
     1261 tcacacatac tagagaaaga ggagaaatac tagatggata cacccaataa agacgattca
     1321 atcatccgct tttcggtttc tttacaacaa aatttattag acgaattaga caaccgcatc
     1381 attaaaaacg gctattcttc tcgctcagaa ttagtgcgcg acatgatcag agaaaaatta
     1441 gtagaagaca attgggccga agataaccct aatgatgaga gcaaaatcgc cgtgcttgtg
     1501 gtgatttatg atcaccacca aagggaattg aaccagcgca tgatagacat tcagcatgcc
     1561 agcgggacgc atgttttatg caccactcac attcacatgg atgagcataa ttgtttagag
     1621 acgattattt tacaaggcaa ttcgtttgaa atccaacgct tgcaactaga aattgggggg
     1681 cttagggggg ttaaattcgc taaattgact aaggcgtcta gctttgaaca caatgaatag
     1741 tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt
     1801 tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg
     1861 gcctttctgc gtttata
//