LOCUS BBa_K1151016 1877 bp DNA linear 26-Nov-2024 DEFINITION LuxI-HpNikR ACCESSION BBa_K1151016 VERSION BBa_K1151016.1 FEATURES Location/Qualifiers promoter 1..253 /label=BBa_K1151005 misc_feature 266..269 /label=conserved RBS 262..273 /label=BBa_B0034 misc_feature 859..890 /label=LVA CDS 280..858 /label=luxI misc_feature 280..897 /label=BBa_C0061 misc_feature 898..922 /label=Help:Barcodes CDS 280..897 /label=BBa_C0061 stem_loop 942..985 /label=stem_loop misc_feature 931..1010 /label=BBa_B0010 terminator 931..1010 /label=BBa_B0010 stem_loop 1026..1045 /label=T7 TE misc_feature 1052..1052 /label=stop polyA_site 1046..1059 /label=polya misc_feature 1019..1059 /label=BBa_B0012 terminator 1019..1059 /label=BBa_B0012 terminator 931..1059 /label=BBa_B0015 misc_feature 262..1059 /label=BBa_K805016 misc_feature 1..1059 /label=BBa_K1151012 CDS 1068..1155 /label=end of LacI coding region (inactive) misc_feature 1068..1267 /label=BBa_R0010 misc_feature 1156..1193 /label=CAP binding site promoter 1204..1209 /label=-35 promoter 1228..1233 /label=-10 misc_feature 1233..1267 /label=LacI binding site misc_feature 1240..1240 /label=start promoter 1068..1267 /label=BBa_R0010 misc_feature 1280..1283 /label=conserved RBS 1276..1287 /label=BBa_B0034 misc_feature 1068..1287 /label=BBa_J04500 CDS 1294..1740 /label=BBa_K1151000 stem_loop 1760..1803 /label=stem_loop misc_feature 1749..1828 /label=BBa_B0010 terminator 1749..1828 /label=BBa_B0010 stem_loop 1844..1863 /label=T7 TE misc_feature 1870..1870 /label=stop polyA_site 1864..1877 /label=polya misc_feature 1837..1877 /label=BBa_B0012 terminator 1837..1877 /label=BBa_B0012 terminator 1749..1877 /label=BBa_B0015 misc_feature 1068..1877 /label=BBa_K1151006 ORIGIN 1 ctcattctcc tattcttaaa gtgtttttcc ttgaagacat aacgaaatca aggttggatg 61 taattgtagc aatgttttga tttactaaga ttaacagaag cgttcattaa ctaattatta 121 tttaaaatga attagtgtta tatttttgaa gcgctataaa agcgttatta gaagcgcgtt 181 taatacccct ttagaattta taaagatcaa aaactcgcaa aaaaaatgag aattaaaatt 241 ggagtgataa tggtactaga gaaagaggag aaatactaga tgactataat gataaaaaaa 301 tcggattttt tggcaattcc atcggaggag tataaaggta ttctaagtct tcgttatcaa 361 gtgtttaagc aaagacttga gtgggactta gttgtagaaa ataaccttga atcagatgag 421 tatgataact caaatgcaga atatatttat gcttgtgatg atactgaaaa tgtaagtgga 481 tgctggcgtt tattacctac aacaggtgat tatatgctga aaagtgtttt tcctgaattg 541 cttggtcaac agagtgctcc caaagatcct aatatagtcg aattaagtcg ttttgctgta 601 ggtaaaaata gctcaaagat aaataactct gctagtgaaa ttacaatgaa actatttgaa 661 gctatatata aacacgctgt tagtcaaggt attacagaat atgtaacagt aacatcaaca 721 gcaatagagc gatttttaaa gcgtattaaa gttccttgtc atcgtattgg agacaaagaa 781 attcatgtat taggtgatac taaatcggtt gtattgtcta tgcctattaa tgaacagttt 841 aaaaaagcag tcttaaatgc tgcaaacgac gaaaactacg ctttagtagc ttaataactc 901 tgatagtgct agtgtagatc tctactagag ccaggcatca aataaaacga aaggctcagt 961 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc 1021 acactggctc accttcgggt gggcctttct gcgtttatat actagagcaa tacgcaaacc 1081 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg 1141 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca 1201 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt 1261 tcacacatac tagagaaaga ggagaaatac tagatggata cacccaataa agacgattca 1321 atcatccgct tttcggtttc tttacaacaa aatttattag acgaattaga caaccgcatc 1381 attaaaaacg gctattcttc tcgctcagaa ttagtgcgcg acatgatcag agaaaaatta 1441 gtagaagaca attgggccga agataaccct aatgatgaga gcaaaatcgc cgtgcttgtg 1501 gtgatttatg atcaccacca aagggaattg aaccagcgca tgatagacat tcagcatgcc 1561 agcgggacgc atgttttatg caccactcac attcacatgg atgagcataa ttgtttagag 1621 acgattattt tacaaggcaa ttcgtttgaa atccaacgct tgcaactaga aattgggggg 1681 cttagggggg ttaaattcgc taaattgact aaggcgtcta gctttgaaca caatgaatag 1741 tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt 1801 tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg 1861 gcctttctgc gtttata //