##gff-version 3
##sequence-region BBa_K1151016 1 1877
BBa_K1151016	.	engineered_region	1	1059	.	+	0	ID=BBa_K1151012;Name=BBa_K1151012
BBa_K1151016	.	promoter	1	253	.	+	0	ID=BBa_K1151005;Name=BBa_K1151005;Parent=BBa_K1151012
BBa_K1151016	.	engineered_region	262	1059	.	+	0	ID=BBa_K805016;Name=BBa_K805016;Parent=BBa_K1151012
BBa_K1151016	.	CDS	280	897	.	+	0	ID=BBa_C0061;Name=BBa_C0061;Parent=BBa_K805016
BBa_K1151016	.	CDS	280	858	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_K1151016	.	engineered_tag	898	922	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_K1151016	.	engineered_region	280	897	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_K1151016	.	tag	859	890	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_K1151016	.	ribosome_entry_site	262	273	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K805016
BBa_K1151016	.	conserved_region	266	269	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1151016	.	terminator	931	1059	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K805016
BBa_K1151016	.	terminator	931	1010	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1151016	.	engineered_region	931	1010	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1151016	.	stem_loop	942	985	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1151016	.	terminator	1019	1059	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1151016	.	stop_codon	1052	1052	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1151016	.	polyA_site	1046	1059	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1151016	.	stem_loop	1026	1045	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1151016	.	engineered_region	1019	1059	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1151016	.	engineered_region	1068	1877	.	+	0	ID=BBa_K1151006;Name=BBa_K1151006
BBa_K1151016	.	engineered_region	1068	1287	.	+	0	ID=BBa_J04500;Name=BBa_J04500;Parent=BBa_K1151006
BBa_K1151016	.	ribosome_entry_site	1276	1287	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J04500
BBa_K1151016	.	conserved_region	1280	1283	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1151016	.	promoter	1068	1267	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_J04500
BBa_K1151016	.	non_covalent_binding_site	1156	1193	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K1151016	.	non_covalent_binding_site	1233	1267	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K1151016	.	promoter	1204	1209	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K1151016	.	engineered_region	1068	1267	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K1151016	.	promoter	1228	1233	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K1151016	.	start_codon	1240	1240	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K1151016	.	CDS	1068	1155	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K1151016	.	CDS	1294	1740	.	+	0	ID=BBa_K1151000;Name=BBa_K1151000;Parent=BBa_K1151006
BBa_K1151016	.	terminator	1749	1877	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K1151006
BBa_K1151016	.	terminator	1749	1828	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1151016	.	engineered_region	1749	1828	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1151016	.	stem_loop	1760	1803	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1151016	.	terminator	1837	1877	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1151016	.	stop_codon	1870	1870	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1151016	.	polyA_site	1864	1877	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1151016	.	stem_loop	1844	1863	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1151016	.	engineered_region	1837	1877	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
>BBa_K1151016
ctcattctcctattcttaaagtgtttttccttgaagacataacgaaatcaaggttggatgtaattgtagcaatgttttg
atttactaagattaacagaagcgttcattaactaattattatttaaaatgaattagtgttatatttttgaagcgctata
aaagcgttattagaagcgcgtttaatacccctttagaatttataaagatcaaaaactcgcaaaaaaaatgagaattaaa
attggagtgataatggtactagagaaagaggagaaatactagatgactataatgataaaaaaatcggattttttggcaa
ttccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgt
agaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgta
agtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacaga
gtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgc
tagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagta
acatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtat
taggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacga
cgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaac
gaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactgg
ctcaccttcgggtgggcctttctgcgtttatatactagagcaatacgcaaaccgcctctccccgcgcgttggccgattc
attaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcac
tcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcac
acatactagagaaagaggagaaatactagatggatacacccaataaagacgattcaatcatccgcttttcggtttcttt
acaacaaaatttattagacgaattagacaaccgcatcattaaaaacggctattcttctcgctcagaattagtgcgcgac
atgatcagagaaaaattagtagaagacaattgggccgaagataaccctaatgatgagagcaaaatcgccgtgcttgtgg
tgatttatgatcaccaccaaagggaattgaaccagcgcatgatagacattcagcatgccagcgggacgcatgttttatg
caccactcacattcacatggatgagcataattgtttagagacgattattttacaaggcaattcgtttgaaatccaacgc
ttgcaactagaaattggggggcttaggggggttaaattcgctaaattgactaaggcgtctagctttgaacacaatgaat
agtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcgg
tgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata