LOCUS       BBa_K1151017            2045 bp    DNA     linear       22-Dec-2024
DEFINITION  GFP-RFP nickel regulated 1 (without terminator)
ACCESSION   BBa_K1151017
VERSION     BBa_K1151017.1  
FEATURES             Location/Qualifiers
     promoter        1..127
                     /label=BBa_K1151003
     RBS             136..148
                     /label=RBS-3\Weak
     RBS             142..145
                     /label=RBS
     misc_feature    136..148
                     /label=BBa_B0032
     RBS             136..148
                     /label=BBa_B0032
     CDS             155..874
                     /label=GFP protein
     CDS             155..874
                     /label=BBa_E0040
     stem_loop       894..937
                     /label=stem_loop
     misc_feature    883..962
                     /label=BBa_B0010
     terminator      883..962
                     /label=BBa_B0010
     stem_loop       978..997
                     /label=T7 TE
     misc_feature    1004..1004
                     /label=stop
     polyA_site      998..1011
                     /label=polya
     misc_feature    971..1011
                     /label=BBa_B0012
     terminator      971..1011
                     /label=BBa_B0012
     misc_feature    136..1011
                     /label=BBa_E0240
     misc_feature    1..1011
                     /label=BBa_K1151009
     promoter        1020..1272
                     /label=BBa_K1151005
     RBS             1281..1292
                     /label=B0034
     CDS             1299..2045
                     /label=mCherry-LVA
     misc_feature    1299..1301
                     /label=Start
     misc_feature    2010..2039
                     /label=ssrA degradation tag: LVA
     misc_feature    2040..2042
                     /label=Stop
     misc_feature    2043..2045
                     /label=Stop
     misc_feature    1281..2045
                     /label=BBa_K773003
     misc_feature    1020..2045
                     /label=BBa_K1151008
ORIGIN
        1 aattcaaacg ctcttatggc caaccattta aatccagttt gtattataat tgttaatttt
       61 aaattaattc aatcatacta gcatgagcgg cttttaacga actcatgcca aaaaaggatt
      121 tttctcatac tagagtcaca caggaaagta ctagatgcgt aaaggagaag aacttttcac
      181 tggagttgtc ccaattcttg ttgaattaga tggtgatgtt aatgggcaca aattttctgt
      241 cagtggagag ggtgaaggtg atgcaacata cggaaaactt acccttaaat ttatttgcac
      301 tactggaaaa ctacctgttc catggccaac acttgtcact actttcggtt atggtgttca
      361 atgctttgcg agatacccag atcatatgaa acagcatgac tttttcaaga gtgccatgcc
      421 cgaaggttat gtacaggaaa gaactatatt tttcaaagat gacgggaact acaagacacg
      481 tgctgaagtc aagtttgaag gtgataccct tgttaataga atcgagttaa aaggtattga
      541 ttttaaagaa gatggaaaca ttcttggaca caaattggaa tacaactata actcacacaa
      601 tgtatacatc atggcagaca aacaaaagaa tggaatcaaa gttaacttca aaattagaca
      661 caacattgaa gatggaagcg ttcaactagc agaccattat caacaaaata ctccaattgg
      721 cgatggccct gtccttttac cagacaacca ttacctgtcc acacaatctg ccctttcgaa
      781 agatcccaac gaaaagagag accacatggt ccttcttgag tttgtaacag ctgctgggat
      841 tacacatggc atggatgaac tatacaaata ataatactag agccaggcat caaataaaac
      901 gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc
      961 tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat atactagagc
     1021 tcattctcct attcttaaag tgtttttcct tgaagacata acgaaatcaa ggttggatgt
     1081 aattgtagca atgttttgat ttactaagat taacagaagc gttcattaac taattattat
     1141 ttaaaatgaa ttagtgttat atttttgaag cgctataaaa gcgttattag aagcgcgttt
     1201 aatacccctt tagaatttat aaagatcaaa aactcgcaaa aaaaatgaga attaaaattg
     1261 gagtgataat ggtactagag aaagaggaga aatactagat ggtgagcaag ggcgaggagg
     1321 ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag ggctccgtga
     1381 acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga
     1441 ccgccaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac atcctgtccc
     1501 ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactact
     1561 tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg
     1621 gcgtggtgac cgtgacccag gactcctcct tgcaggacgg cgagttcatc tacaaggtga
     1681 agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag accatgggct
     1741 gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc gagatcaagc
     1801 agaggctgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc acctacaagg
     1861 ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa catcaagttg gacatcacct
     1921 cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc cgccactcca
     1981 ccggcggcat ggacgagctg tacaaggcag caaacgacga aaactacgct ctagtagctt
     2041 aataa
//