##gff-version 3
##sequence-region BBa_K1151017 1 2045
BBa_K1151017	.	engineered_region	1	1011	.	+	0	ID=BBa_K1151009;Name=BBa_K1151009
BBa_K1151017	.	engineered_region	136	1011	.	+	0	ID=BBa_E0240;Name=BBa_E0240;Parent=BBa_K1151009
BBa_K1151017	.	CDS	155	874	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_E0240
BBa_K1151017	.	CDS	155	874	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K1151017	.	ribosome_entry_site	136	148	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_E0240
BBa_K1151017	.	ribosome_entry_site	142	145	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K1151017	.	engineered_region	136	148	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K1151017	.	ribosome_entry_site	136	148	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K1151017	.	terminator	883	962	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0240
BBa_K1151017	.	engineered_region	883	962	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1151017	.	stem_loop	894	937	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1151017	.	terminator	971	1011	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0240
BBa_K1151017	.	engineered_region	971	1011	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1151017	.	stop_codon	1004	1004	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1151017	.	polyA_site	998	1011	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1151017	.	stem_loop	978	997	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1151017	.	promoter	1	127	.	+	0	ID=BBa_K1151003;Name=BBa_K1151003;Parent=BBa_K1151009
BBa_K1151017	.	engineered_region	1020	2045	.	+	0	ID=BBa_K1151008;Name=BBa_K1151008
BBa_K1151017	.	engineered_region	1281	2045	.	+	0	ID=BBa_K773003;Name=BBa_K773003;Parent=BBa_K1151008
BBa_K1151017	.	stop_codon	2043	2045	.	+	0	ID=annotation2209513;Name=Stop;Parent=BBa_K773003
BBa_K1151017	.	tag	2010	2039	.	+	0	ID=annotation2209511;Name=ssrA degradation tag: LVA;Parent=BBa_K773003
BBa_K1151017	.	CDS	1299	2045	.	+	0	ID=annotation2209509;Name=mCherry-LVA;Parent=BBa_K773003
BBa_K1151017	.	ribosome_entry_site	1281	1292	.	+	0	ID=annotation2209508;Name=B0034;Parent=BBa_K773003
BBa_K1151017	.	start_codon	1299	1301	.	+	0	ID=annotation2209510;Name=Start;Parent=BBa_K773003
BBa_K1151017	.	stop_codon	2040	2042	.	+	0	ID=annotation2209512;Name=Stop;Parent=BBa_K773003
BBa_K1151017	.	promoter	1020	1272	.	+	0	ID=BBa_K1151005;Name=BBa_K1151005;Parent=BBa_K1151008
>BBa_K1151017
aattcaaacgctcttatggccaaccatttaaatccagtttgtattataattgttaattttaaattaattcaatcatact
agcatgagcggcttttaacgaactcatgccaaaaaaggatttttctcatactagagtcacacaggaaagtactagatgc
gtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttc
tgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacct
gttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagc
atgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaa
gacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagat
ggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatg
gaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatac
tccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaac
gaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaat
aataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgt
cggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagctcattct
cctattcttaaagtgtttttccttgaagacataacgaaatcaaggttggatgtaattgtagcaatgttttgatttacta
agattaacagaagcgttcattaactaattattatttaaaatgaattagtgttatatttttgaagcgctataaaagcgtt
attagaagcgcgtttaatacccctttagaatttataaagatcaaaaactcgcaaaaaaaatgagaattaaaattggagt
gataatggtactagagaaagaggagaaatactagatggtgagcaagggcgaggaggataacatggccatcatcaaggag
ttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcc
cctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccc
tcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgag
ggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacg
gcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatggg
ctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaag
gacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacg
tcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgcca
ctccaccggcggcatggacgagctgtacaaggcagcaaacgacgaaaactacgctctagtagcttaataa