LOCUS       BBa_K1151018            2077 bp    DNA     linear       22-Nov-2024
DEFINITION  GFP-RFP nickel regulated 2 (without terminator)
ACCESSION   BBa_K1151018
VERSION     BBa_K1151018.1  
FEATURES             Location/Qualifiers
     promoter        1..159
                     /label=BBa_K1151004
     RBS             168..180
                     /label=RBS-3\Weak
     RBS             174..177
                     /label=RBS
     misc_feature    168..180
                     /label=BBa_B0032
     RBS             168..180
                     /label=BBa_B0032
     CDS             187..906
                     /label=GFP protein
     CDS             187..906
                     /label=BBa_E0040
     stem_loop       926..969
                     /label=stem_loop
     misc_feature    915..994
                     /label=BBa_B0010
     terminator      915..994
                     /label=BBa_B0010
     stem_loop       1010..1029
                     /label=T7 TE
     misc_feature    1036..1036
                     /label=stop
     polyA_site      1030..1043
                     /label=polya
     misc_feature    1003..1043
                     /label=BBa_B0012
     terminator      1003..1043
                     /label=BBa_B0012
     misc_feature    168..1043
                     /label=BBa_E0240
     misc_feature    1..1043
                     /label=BBa_K1151010
     promoter        1052..1304
                     /label=BBa_K1151005
     RBS             1313..1324
                     /label=B0034
     CDS             1331..2077
                     /label=mCherry-LVA
     misc_feature    1331..1333
                     /label=Start
     misc_feature    2042..2071
                     /label=ssrA degradation tag: LVA
     misc_feature    2072..2074
                     /label=Stop
     misc_feature    2075..2077
                     /label=Stop
     misc_feature    1313..2077
                     /label=BBa_K773003
     misc_feature    1052..2077
                     /label=BBa_K1151008
ORIGIN
        1 actggattta aatggttggc cataagagcg tttgaatttg attgtaatta ttagcttaat
       61 catcattgac ttgttattat taaaacaata caatcaacaa accaacattc ttttatcatt
      121 ttggagcatt tatgcgaacg gatgcttctt atagggtgct actagagtca cacaggaaag
      181 tactagatgc gtaaaggaga agaacttttc actggagttg tcccaattct tgttgaatta
      241 gatggtgatg ttaatgggca caaattttct gtcagtggag agggtgaagg tgatgcaaca
      301 tacggaaaac ttacccttaa atttatttgc actactggaa aactacctgt tccatggcca
      361 acacttgtca ctactttcgg ttatggtgtt caatgctttg cgagataccc agatcatatg
      421 aaacagcatg actttttcaa gagtgccatg cccgaaggtt atgtacagga aagaactata
      481 tttttcaaag atgacgggaa ctacaagaca cgtgctgaag tcaagtttga aggtgatacc
      541 cttgttaata gaatcgagtt aaaaggtatt gattttaaag aagatggaaa cattcttgga
      601 cacaaattgg aatacaacta taactcacac aatgtataca tcatggcaga caaacaaaag
      661 aatggaatca aagttaactt caaaattaga cacaacattg aagatggaag cgttcaacta
      721 gcagaccatt atcaacaaaa tactccaatt ggcgatggcc ctgtcctttt accagacaac
      781 cattacctgt ccacacaatc tgccctttcg aaagatccca acgaaaagag agaccacatg
      841 gtccttcttg agtttgtaac agctgctggg attacacatg gcatggatga actatacaaa
      901 taataatact agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt
      961 tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc
     1021 gggtgggcct ttctgcgttt atatactaga gctcattctc ctattcttaa agtgtttttc
     1081 cttgaagaca taacgaaatc aaggttggat gtaattgtag caatgttttg atttactaag
     1141 attaacagaa gcgttcatta actaattatt atttaaaatg aattagtgtt atatttttga
     1201 agcgctataa aagcgttatt agaagcgcgt ttaatacccc tttagaattt ataaagatca
     1261 aaaactcgca aaaaaaatga gaattaaaat tggagtgata atggtactag agaaagagga
     1321 gaaatactag atggtgagca agggcgagga ggataacatg gccatcatca aggagttcat
     1381 gcgcttcaag gtgcacatgg agggctccgt gaacggccac gagttcgaga tcgagggcga
     1441 gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggtgg
     1501 ccccctgccc ttcgcctggg acatcctgtc ccctcagttc atgtacggct ccaaggccta
     1561 cgtgaagcac cccgccgaca tccccgacta cttgaagctg tccttccccg agggcttcaa
     1621 gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc
     1681 cttgcaggac ggcgagttca tctacaaggt gaagctgcgc ggcaccaact tcccctccga
     1741 cggccccgta atgcagaaga agaccatggg ctgggaggcc tcctccgagc ggatgtaccc
     1801 cgaggacggc gccctgaagg gcgagatcaa gcagaggctg aagctgaagg acggcggcca
     1861 ctacgacgct gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc
     1921 ctacaacgtc aacatcaagt tggacatcac ctcccacaac gaggactaca ccatcgtgga
     1981 acagtacgaa cgcgccgagg gccgccactc caccggcggc atggacgagc tgtacaaggc
     2041 agcaaacgac gaaaactacg ctctagtagc ttaataa
//