LOCUS       BBa_K1151019            2166 bp    DNA     linear       22-Dec-2024
DEFINITION  GFP-RFP nickel regulated 3 (without terminator)
ACCESSION   BBa_K1151019
VERSION     BBa_K1151019.1  
FEATURES             Location/Qualifiers
     promoter        1..248
                     /label=BBa_K1151002
     RBS             257..269
                     /label=RBS-3\Weak
     RBS             263..266
                     /label=RBS
     misc_feature    257..269
                     /label=BBa_B0032
     RBS             257..269
                     /label=BBa_B0032
     CDS             276..995
                     /label=GFP protein
     CDS             276..995
                     /label=BBa_E0040
     stem_loop       1015..1058
                     /label=stem_loop
     misc_feature    1004..1083
                     /label=BBa_B0010
     terminator      1004..1083
                     /label=BBa_B0010
     stem_loop       1099..1118
                     /label=T7 TE
     misc_feature    1125..1125
                     /label=stop
     polyA_site      1119..1132
                     /label=polya
     misc_feature    1092..1132
                     /label=BBa_B0012
     terminator      1092..1132
                     /label=BBa_B0012
     misc_feature    257..1132
                     /label=BBa_E0240
     misc_feature    1..1132
                     /label=BBa_K1151011
     promoter        1141..1393
                     /label=BBa_K1151005
     RBS             1402..1413
                     /label=B0034
     CDS             1420..2166
                     /label=mCherry-LVA
     misc_feature    1420..1422
                     /label=Start
     misc_feature    2131..2160
                     /label=ssrA degradation tag: LVA
     misc_feature    2161..2163
                     /label=Stop
     misc_feature    2164..2166
                     /label=Stop
     misc_feature    1402..2166
                     /label=BBa_K773003
     misc_feature    1141..2166
                     /label=BBa_K1151008
ORIGIN
        1 tgagaaaaat ccttttttgg catgagttcg ttaaaagccg ctcatgctag tatgattgaa
       61 ttaatttaaa attaacaatt ataatacaaa ctggatttaa atggttggcc ataagagcgt
      121 ttgaatttga ttgtaattat tagcttaatc atcattgact tgttattatt aaaacaatac
      181 aatcaacaaa ccaacattct tttatcattt tggagcattt atgcgaacgg atgcttctta
      241 tagggtgcta ctagagtcac acaggaaagt actagatgcg taaaggagaa gaacttttca
      301 ctggagttgt cccaattctt gttgaattag atggtgatgt taatgggcac aaattttctg
      361 tcagtggaga gggtgaaggt gatgcaacat acggaaaact tacccttaaa tttatttgca
      421 ctactggaaa actacctgtt ccatggccaa cacttgtcac tactttcggt tatggtgttc
      481 aatgctttgc gagataccca gatcatatga aacagcatga ctttttcaag agtgccatgc
      541 ccgaaggtta tgtacaggaa agaactatat ttttcaaaga tgacgggaac tacaagacac
      601 gtgctgaagt caagtttgaa ggtgataccc ttgttaatag aatcgagtta aaaggtattg
      661 attttaaaga agatggaaac attcttggac acaaattgga atacaactat aactcacaca
      721 atgtatacat catggcagac aaacaaaaga atggaatcaa agttaacttc aaaattagac
      781 acaacattga agatggaagc gttcaactag cagaccatta tcaacaaaat actccaattg
      841 gcgatggccc tgtcctttta ccagacaacc attacctgtc cacacaatct gccctttcga
      901 aagatcccaa cgaaaagaga gaccacatgg tccttcttga gtttgtaaca gctgctggga
      961 ttacacatgg catggatgaa ctatacaaat aataatacta gagccaggca tcaaataaaa
     1021 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct
     1081 ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta tatactagag
     1141 ctcattctcc tattcttaaa gtgtttttcc ttgaagacat aacgaaatca aggttggatg
     1201 taattgtagc aatgttttga tttactaaga ttaacagaag cgttcattaa ctaattatta
     1261 tttaaaatga attagtgtta tatttttgaa gcgctataaa agcgttatta gaagcgcgtt
     1321 taatacccct ttagaattta taaagatcaa aaactcgcaa aaaaaatgag aattaaaatt
     1381 ggagtgataa tggtactaga gaaagaggag aaatactaga tggtgagcaa gggcgaggag
     1441 gataacatgg ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga gggctccgtg
     1501 aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag
     1561 accgccaagc tgaaggtgac caagggtggc cccctgccct tcgcctggga catcctgtcc
     1621 cctcagttca tgtacggctc caaggcctac gtgaagcacc ccgccgacat ccccgactac
     1681 ttgaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc
     1741 ggcgtggtga ccgtgaccca ggactcctcc ttgcaggacg gcgagttcat ctacaaggtg
     1801 aagctgcgcg gcaccaactt cccctccgac ggccccgtaa tgcagaagaa gaccatgggc
     1861 tgggaggcct cctccgagcg gatgtacccc gaggacggcg ccctgaaggg cgagatcaag
     1921 cagaggctga agctgaagga cggcggccac tacgacgctg aggtcaagac cacctacaag
     1981 gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt ggacatcacc
     2041 tcccacaacg aggactacac catcgtggaa cagtacgaac gcgccgaggg ccgccactcc
     2101 accggcggca tggacgagct gtacaaggca gcaaacgacg aaaactacgc tctagtagct
     2161 taataa
//