##gff-version 3
##sequence-region BBa_K1151023 1 2015
BBa_K1151023	.	engineered_region	819	2015	.	+	0	ID=BBa_K1151014;Name=BBa_K1151014
BBa_K1151023	.	engineered_region	1080	2015	.	+	0	ID=BBa_I0462;Name=BBa_I0462;Parent=BBa_K1151014
BBa_K1151023	.	terminator	1975	2015	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I0462
BBa_K1151023	.	stop_codon	2008	2008	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1151023	.	polyA_site	2002	2015	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1151023	.	stem_loop	1982	2001	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1151023	.	engineered_region	1975	2015	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1151023	.	CDS	1098	1853	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_I0462
BBa_K1151023	.	engineered_tag	1854	1878	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K1151023	.	engineered_region	1098	1853	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K1151023	.	engineered_region	1098	1099	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K1151023	.	point_mutation	1271	1271	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K1151023	.	CDS	1098	1847	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K1151023	.	point_mutation	1589	1589	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K1151023	.	terminator	1887	1966	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I0462
BBa_K1151023	.	engineered_region	1887	1966	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1151023	.	stem_loop	1898	1941	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1151023	.	ribosome_entry_site	1080	1091	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I0462
BBa_K1151023	.	conserved_region	1084	1087	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1151023	.	promoter	819	1071	.	+	0	ID=BBa_K1151005;Name=BBa_K1151005;Parent=BBa_K1151014
BBa_K1151023	.	engineered_region	1	810	.	+	0	ID=BBa_K1151006;Name=BBa_K1151006
BBa_K1151023	.	engineered_region	1	220	.	+	0	ID=BBa_J04500;Name=BBa_J04500;Parent=BBa_K1151006
BBa_K1151023	.	ribosome_entry_site	209	220	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J04500
BBa_K1151023	.	conserved_region	213	216	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1151023	.	promoter	1	200	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_J04500
BBa_K1151023	.	non_covalent_binding_site	89	126	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K1151023	.	non_covalent_binding_site	166	200	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K1151023	.	promoter	137	142	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K1151023	.	engineered_region	1	200	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K1151023	.	promoter	161	166	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K1151023	.	start_codon	173	173	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K1151023	.	CDS	1	88	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K1151023	.	CDS	227	673	.	+	0	ID=BBa_K1151000;Name=BBa_K1151000;Parent=BBa_K1151006
BBa_K1151023	.	terminator	682	810	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K1151006
BBa_K1151023	.	terminator	682	761	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1151023	.	engineered_region	682	761	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1151023	.	stem_loop	693	736	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1151023	.	terminator	770	810	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1151023	.	stop_codon	803	803	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1151023	.	polyA_site	797	810	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1151023	.	stem_loop	777	796	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1151023	.	engineered_region	770	810	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
>BBa_K1151023
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatggatacacc
caataaagacgattcaatcatccgcttttcggtttctttacaacaaaatttattagacgaattagacaaccgcatcatt
aaaaacggctattcttctcgctcagaattagtgcgcgacatgatcagagaaaaattagtagaagacaattgggccgaag
ataaccctaatgatgagagcaaaatcgccgtgcttgtggtgatttatgatcaccaccaaagggaattgaaccagcgcat
gatagacattcagcatgccagcgggacgcatgttttatgcaccactcacattcacatggatgagcataattgtttagag
acgattattttacaaggcaattcgtttgaaatccaacgcttgcaactagaaattggggggcttaggggggttaaattcg
ctaaattgactaaggcgtctagctttgaacacaatgaatagtactagagccaggcatcaaataaaacgaaaggctcagt
cgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcggg
tgggcctttctgcgtttatatactagagctcattctcctattcttaaagtgtttttccttgaagacataacgaaatcaa
ggttggatgtaattgtagcaatgttttgatttactaagattaacagaagcgttcattaactaattattatttaaaatga
attagtgttatatttttgaagcgctataaaagcgttattagaagcgcgtttaatacccctttagaatttataaagatca
aaaactcgcaaaaaaaatgagaattaaaattggagtgataatggtactagagaaagaggagaaatactagatgaaaaac
ataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttat
ctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatat
ttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagta
gattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatg
taattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaat
gcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaatt
gttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaa
aagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgt
cactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaaca
ggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatca
aataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagt
cacactggctcaccttcgggtgggcctttctgcgtttata