BBa_K1151000
1
BBa_K1151000
Nickel-responsive pleiotropic regulator (HpNikR)
2013-09-20T11:00:00Z
2015-05-08T01:09:28Z
It was amplified by PCR from the genomic sequence of Helicobacter pylori G27.
The HpNikR protein is a pleiotropic regulator from Helicobacter pylori. In presence of nickel it can acts as an activator or a repressor depending of the specific promoter that contains its operator sites. Each monomer consists in 133aa for a molecular weight of approximately 17.2 kDa. Apo-HpNikR is a tetramer able to bind up to six Ni2+ ions and then a conformational change allows it to activate or repress trascription.
false
false
_1463_
0
16632
9
Not in stock
false
It was inserted in a pET-15b plasmid vector, in order to have a better storage.
false
Emanuele Rizzo
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1690
1
polya
range1690
1
28
41
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1686
1
T7 TE
range1686
1
8
27
annotation1687
1
stop
range1687
1
34
34
BBa_K1151026
1
BBa_K1151026
HpNikR-TER
2013-09-20T11:00:00Z
2015-05-08T01:09:29Z
None
It's a biobrick composed of HpNikR-TER.
false
false
_1463_
0
16632
9
It's complicated
false
None
false
Emanuele Rizzo
component2357442
1
BBa_K1151000
component2357449
1
BBa_B0015
annotation2357449
1
BBa_B0015
range2357449
1
456
584
annotation2357442
1
BBa_K1151000
range2357442
1
1
447
BBa_B0015
1
BBa_B0015
double terminator (B0010-B0012)
2003-07-16T11:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Double terminator consisting of BBa_B0010 and BBa_B0012
false
true
_1_
0
24
7
In stock
false
true
Reshma Shetty
component1916612
1
BBa_B0012
component1916610
1
BBa_B0010
annotation1916612
1
BBa_B0012
range1916612
1
89
129
annotation1916610
1
BBa_B0010
range1916610
1
1
80
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation4184
1
stem_loop
range4184
1
12
55
annotation7018
1
BBa_B0010
range7018
1
1
80
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_K1151026_sequence
1
atggatacacccaataaagacgattcaatcatccgcttttcggtttctttacaacaaaatttattagacgaattagacaaccgcatcattaaaaacggctattcttctcgctcagaattagtgcgcgacatgatcagagaaaaattagtagaagacaattgggccgaagataaccctaatgatgagagcaaaatcgccgtgcttgtggtgatttatgatcaccaccaaagggaattgaaccagcgcatgatagacattcagcatgccagcgggacgcatgttttatgcaccactcacattcacatggatgagcataattgtttagagacgattattttacaaggcaattcgtttgaaatccaacgcttgcaactagaaattggggggcttaggggggttaaattcgctaaattgactaaggcgtctagctttgaacacaatgaatagtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_K1151000_sequence
1
atggatacacccaataaagacgattcaatcatccgcttttcggtttctttacaacaaaatttattagacgaattagacaaccgcatcattaaaaacggctattcttctcgctcagaattagtgcgcgacatgatcagagaaaaattagtagaagacaattgggccgaagataaccctaatgatgagagcaaaatcgccgtgcttgtggtgatttatgatcaccaccaaagggaattgaaccagcgcatgatagacattcagcatgccagcgggacgcatgttttatgcaccactcacattcacatggatgagcataattgtttagagacgattattttacaaggcaattcgtttgaaatccaacgcttgcaactagaaattggggggcttaggggggttaaattcgctaaattgactaaggcgtctagctttgaacacaatgaatag
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_B0015_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z