##gff-version 3
##sequence-region BBa_K1151028 1 2077
BBa_K1151028	.	engineered_region	1052	2077	.	+	0	ID=BBa_K1151008;Name=BBa_K1151008
BBa_K1151028	.	engineered_region	1313	2077	.	+	0	ID=BBa_K773003;Name=BBa_K773003;Parent=BBa_K1151008
BBa_K1151028	.	stop_codon	2075	2077	.	+	0	ID=annotation2209513;Name=Stop;Parent=BBa_K773003
BBa_K1151028	.	CDS	1331	2077	.	+	0	ID=annotation2209509;Name=mCherry-LVA;Parent=BBa_K773003
BBa_K1151028	.	tag	2042	2071	.	+	0	ID=annotation2209511;Name=ssrA degradation tag: LVA;Parent=BBa_K773003
BBa_K1151028	.	start_codon	1331	1333	.	+	0	ID=annotation2209510;Name=Start;Parent=BBa_K773003
BBa_K1151028	.	stop_codon	2072	2074	.	+	0	ID=annotation2209512;Name=Stop;Parent=BBa_K773003
BBa_K1151028	.	ribosome_entry_site	1313	1324	.	+	0	ID=annotation2209508;Name=B0034;Parent=BBa_K773003
BBa_K1151028	.	promoter	1052	1304	.	+	0	ID=BBa_K1151005;Name=BBa_K1151005;Parent=BBa_K1151008
BBa_K1151028	.	engineered_region	1	1043	.	+	0	ID=BBa_K1151010;Name=BBa_K1151010
BBa_K1151028	.	engineered_region	168	1043	.	+	0	ID=BBa_E0240;Name=BBa_E0240;Parent=BBa_K1151010
BBa_K1151028	.	terminator	915	994	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0240
BBa_K1151028	.	stem_loop	926	969	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1151028	.	engineered_region	915	994	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1151028	.	terminator	1003	1043	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0240
BBa_K1151028	.	stem_loop	1010	1029	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1151028	.	polyA_site	1030	1043	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1151028	.	stop_codon	1036	1036	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1151028	.	engineered_region	1003	1043	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1151028	.	CDS	187	906	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_E0240
BBa_K1151028	.	CDS	187	906	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K1151028	.	ribosome_entry_site	168	180	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_E0240
BBa_K1151028	.	engineered_region	168	180	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K1151028	.	ribosome_entry_site	174	177	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K1151028	.	ribosome_entry_site	168	180	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K1151028	.	promoter	1	159	.	+	0	ID=BBa_K1151004;Name=BBa_K1151004;Parent=BBa_K1151010
>BBa_K1151028
actggatttaaatggttggccataagagcgtttgaatttgattgtaattattagcttaatcatcattgacttgttatta
ttaaaacaatacaatcaacaaaccaacattcttttatcattttggagcatttatgcgaacggatgcttcttatagggtg
ctactagagtcacacaggaaagtactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaa
ttagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttaccc
ttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatg
ctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaaga
actatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaa
tcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaa
tgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagc
gttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgt
ccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgg
gattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaa
agactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtggg
cctttctgcgtttatatactagagctcattctcctattcttaaagtgtttttccttgaagacataacgaaatcaaggtt
ggatgtaattgtagcaatgttttgatttactaagattaacagaagcgttcattaactaattattatttaaaatgaatta
gtgttatatttttgaagcgctataaaagcgttattagaagcgcgtttaatacccctttagaatttataaagatcaaaaa
ctcgcaaaaaaaatgagaattaaaattggagtgataatggtactagagaaagaggagaaatactagatggtgagcaagg
gcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacga
gttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggc
cccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgaca
tccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggt
gaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctcc
gacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctga
agggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaa
gaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatc
gtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaaggcagcaaacgacgaaa
actacgctctagtagcttaataa