BBa_K1151029
type: ComponentDefinition
persistent identity: BBa K1151029
display id: BBa_K1151029
version: 1
was generated by: iGEM to SBOL conversion
description: pnikR-RBS-GFP-TER-pUreA-RBS-RFP (LVAtag)
created: 2013-09-23T11:00:00Z
modified: 2015-05-08T01:09:29Z
mutable provenance: none
owned by: james
owned by: myers
mutable description: Fusion of BBa_K1151009 and BBa_K1151008
discontinued: false
dominant: false
group u list: _1463_
m user id: 0
owner id: 16629
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: none
star: false
creator: Davide Magr??
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1151009
component: BBa_K1151008
sequence annotation: BBa_K1151009
sequence annotation: BBa_K1151008
sequence: https://synbiohub.org/public/igem/BBa_K1151029_sequence/1

BBa_K1151009
type: Component
persistent identity: component2361207
display id: component2361207
version: 1
top level: BBa_K1151029
definition: BBa_K1151009
access: public

BBa_K1151008
type: Component
persistent identity: component2361216
display id: component2361216
version: 1
top level: BBa_K1151029
definition: BBa_K1151008
access: public

BBa_K1151009
type: SequenceAnnotation
persistent identity: annotation2361207
display id: annotation2361207
version: 1
top level: BBa_K1151029
location: https://synbiohub.org/public/igem/BBa_K1151029/annotation2361207/range2361207/1
component: BBa_K1151009

https://synbiohub.org/public/igem/BBa_K1151029/annotation2361207/range2361207/1
type: Range
persistent identity: range2361207
display id: range2361207
version: 1
top level: BBa_K1151029
direction: direction/reverse
start: 1
end: 1011
orientation: inline

BBa_K1151008
type: SequenceAnnotation
persistent identity: annotation2361216
display id: annotation2361216
version: 1
top level: BBa_K1151029
location: https://synbiohub.org/public/igem/BBa_K1151029/annotation2361216/range2361216/1
component: BBa_K1151008

https://synbiohub.org/public/igem/BBa_K1151029/annotation2361216/range2361216/1
type: Range
persistent identity: range2361216
display id: range2361216
version: 1
top level: BBa_K1151029
direction: direction/reverse
start: 1020
end: 2045
orientation: inline

BBa_K1151008
type: ComponentDefinition
persistent identity: BBa K1151008
display id: BBa_K1151008
version: 1
was generated by: iGEM to SBOL conversion
description: RFP nickel regulated
created: 2013-09-22T11:00:00Z
modified: 2015-05-08T01:09:29Z
mutable provenance: pUreA was synthesized starting from the genome sequence of H. pylori, instead the other parts coming from iGEM kit.
owned by: james
owned by: myers
mutable description: This part was built as a ligation product between the promoter of our design, pUreA, RBS (B0034), RFP (LVA tag) and the terminator B0015.
discontinued: false
dominant: false
experience: experience/None
group u list: _1463_
m user id: 0
owner id: 16631
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: None
star: false
creator: Gian Piero Lazzari
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K773003
component: BBa_K1151005
sequence annotation: BBa_K1151005
sequence annotation: BBa_K773003
sequence: https://synbiohub.org/public/igem/BBa_K1151008_sequence/1

BBa_K773003
type: Component
persistent identity: component2360860
display id: component2360860
version: 1
top level: BBa_K1151008
definition: BBa_K773003
access: public

BBa_K1151005
type: Component
persistent identity: component2360853
display id: component2360853
version: 1
top level: BBa_K1151008
definition: BBa_K1151005
access: public

BBa_K1151005
type: SequenceAnnotation
persistent identity: annotation2360853
display id: annotation2360853
version: 1
top level: BBa_K1151008
location: https://synbiohub.org/public/igem/BBa_K1151008/annotation2360853/range2360853/1
component: BBa_K1151005

https://synbiohub.org/public/igem/BBa_K1151008/annotation2360853/range2360853/1
type: Range
persistent identity: range2360853
display id: range2360853
version: 1
top level: BBa_K1151008
direction: direction/reverse
start: 1
end: 253
orientation: inline

BBa_K773003
type: SequenceAnnotation
persistent identity: annotation2360860
display id: annotation2360860
version: 1
top level: BBa_K1151008
location: https://synbiohub.org/public/igem/BBa_K1151008/annotation2360860/range2360860/1
component: BBa_K773003

https://synbiohub.org/public/igem/BBa_K1151008/annotation2360860/range2360860/1
type: Range
persistent identity: range2360860
display id: range2360860
version: 1
top level: BBa_K1151008
direction: direction/reverse
start: 262
end: 1026
orientation: inline

BBa_K1151009
type: ComponentDefinition
persistent identity: BBa K1151009
display id: BBa_K1151009
version: 1
was generated by: iGEM to SBOL conversion
description: GFP nickel regulated 1
created: 2013-09-22T11:00:00Z
modified: 2015-05-08T01:09:29Z
mutable provenance: None
owned by: james
owned by: myers
mutable description: This is a nickel sensitive system driven by the promoter pnikR (BBa_K1151003) with a GFP reporter. Normally the production of GFP is switched on, it is possible to repress the GFP expression using nickel ions in medium and in presence of HpNikR (BBa_K1151000) as trascription factor.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1463_
m user id: 0
owner id: 16631
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: None
star: false
creator: Gian Piero Lazzari
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_K1151003
component: BBa_E0240
sequence annotation: BBa_K1151003
sequence annotation: BBa_E0240
sequence: https://synbiohub.org/public/igem/BBa_K1151009_sequence/1

BBa_K1151003
type: Component
persistent identity: component2358764
display id: component2358764
version: 1
top level: BBa_K1151009
definition: BBa_K1151003
access: public

BBa_E0240
type: Component
persistent identity: component2358775
display id: component2358775
version: 1
top level: BBa_K1151009
definition: GFP report
access: public

BBa_K1151003
type: SequenceAnnotation
persistent identity: annotation2358764
display id: annotation2358764
version: 1
top level: BBa_K1151009
location: https://synbiohub.org/public/igem/BBa_K1151009/annotation2358764/range2358764/1
component: BBa_K1151003

https://synbiohub.org/public/igem/BBa_K1151009/annotation2358764/range2358764/1
type: Range
persistent identity: range2358764
display id: range2358764
version: 1
top level: BBa_K1151009
direction: direction/reverse
start: 1
end: 127
orientation: inline

BBa_E0240
type: SequenceAnnotation
persistent identity: annotation2358775
display id: annotation2358775
version: 1
top level: BBa_K1151009
location: https://synbiohub.org/public/igem/BBa_K1151009/annotation2358775/range2358775/1
component: BBa_E0240

https://synbiohub.org/public/igem/BBa_K1151009/annotation2358775/range2358775/1
type: Range
persistent identity: range2358775
display id: range2358775
version: 1
top level: BBa_K1151009
direction: direction/reverse
start: 136
end: 1011
orientation: inline

GFP report
type: ComponentDefinition
persistent identity: BBa E0240
display id: BBa_E0240
version: 1
was generated by: iGEM to SBOL conversion
description: GFP generator
created: 2004-10-17T11:00:00Z
modified: 2015-08-31T04:07:26Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: B0032.E0040.B0015
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Jennifer Braff
type: DnaRegion
role: SO:0000804
role: partType/Reporter
component: BBa_B0032
component: BBa_B0010
component: BBa_E0040
component: BBa_B0012
sequence annotation: BBa_B0032
sequence annotation: BBa_E0040
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_E0240_sequence/1

BBa_B0032
type: Component
persistent identity: component1249213
display id: component1249213
version: 1
top level: GFP report
definition: BBa_B0032
access: public

BBa_B0010
type: Component
persistent identity: component1249221
display id: component1249221
version: 1
top level: GFP report
definition: BBa_B0010
access: public

BBa_E0040
type: Component
persistent identity: component1249216
display id: component1249216
version: 1
top level: GFP report
definition: GFP
access: public

BBa_B0012
type: Component
persistent identity: component1249231
display id: component1249231
version: 1
top level: GFP report
definition: BBa_B0012
access: public

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1249213
display id: annotation1249213
version: 1
top level: GFP report
location: https://synbiohub.org/public/igem/BBa_E0240/annotation1249213/range1249213/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_E0240/annotation1249213/range1249213/1
type: Range
persistent identity: range1249213
display id: range1249213
version: 1
top level: GFP report
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1249216
display id: annotation1249216
version: 1
top level: GFP report
location: https://synbiohub.org/public/igem/BBa_E0240/annotation1249216/range1249216/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_E0240/annotation1249216/range1249216/1
type: Range
persistent identity: range1249216
display id: range1249216
version: 1
top level: GFP report
direction: direction/forward
start: 20
end: 739
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1249221
display id: annotation1249221
version: 1
top level: GFP report
location: https://synbiohub.org/public/igem/BBa_E0240/annotation1249221/range1249221/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_E0240/annotation1249221/range1249221/1
type: Range
persistent identity: range1249221
display id: range1249221
version: 1
top level: GFP report
direction: direction/forward
start: 748
end: 827
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1249231
display id: annotation1249231
version: 1
top level: GFP report
location: https://synbiohub.org/public/igem/BBa_E0240/annotation1249231/range1249231/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_E0240/annotation1249231/range1249231/1
type: Range
persistent identity: range1249231
display id: range1249231
version: 1
top level: GFP report
direction: direction/forward
start: 836
end: 876
orientation: inline

BBa_K773003
type: ComponentDefinition
persistent identity: BBa K773003
display id: BBa_K773003
version: 1
was generated by: iGEM to SBOL conversion
description: mCherry-LVA
created: 2012-09-30T11:00:00Z
modified: 2015-05-08T01:13:15Z
mutable provenance: This part was modified from a plasmid provided by the Murray lab at Caltech.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: This part contains the fluorescent protein mCherry attached to a degradation tag. It will decrease the amount of fluorescent protein over time.
discontinued: false
dominant: false
group u list: _1025_
m user id: 0
owner id: 11646
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: We modified the AAV sequence to LVA with PCR.
star: false
creator: Katie Knister
type: DnaRegion
role: SO:0000804
role: partType/Translational Unit
sequence annotation: B0034
sequence annotation: mCherry-LVA
sequence annotation: Start
sequence annotation: ssrA degradation tag: LVA
sequence annotation: Stop
sequence: https://synbiohub.org/public/igem/BBa_K773003_sequence/1

B0034
type: SequenceAnnotation
persistent identity: annotation2209508
display id: annotation2209508
version: 1
top level: BBa_K773003
location: https://synbiohub.org/public/igem/BBa_K773003/annotation2209508/range2209508/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_K773003/annotation2209508/range2209508/1
type: Range
persistent identity: range2209508
display id: range2209508
version: 1
top level: BBa_K773003
direction: direction/forward
start: 1
end: 12
orientation: inline

mCherry-LVA
type: SequenceAnnotation
persistent identity: annotation2209509
display id: annotation2209509
version: 1
top level: BBa_K773003
location: https://synbiohub.org/public/igem/BBa_K773003/annotation2209509/range2209509/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K773003/annotation2209509/range2209509/1
type: Range
persistent identity: range2209509
display id: range2209509
version: 1
top level: BBa_K773003
direction: direction/forward
start: 19
end: 765
orientation: inline

Start
type: SequenceAnnotation
persistent identity: annotation2209510
display id: annotation2209510
version: 1
top level: BBa_K773003
location: https://synbiohub.org/public/igem/BBa_K773003/annotation2209510/range2209510/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_K773003/annotation2209510/range2209510/1
type: Range
persistent identity: range2209510
display id: range2209510
version: 1
top level: BBa_K773003
direction: direction/forward
start: 19
end: 21
orientation: inline

ssrA degradation tag: LVA
type: SequenceAnnotation
persistent identity: annotation2209511
display id: annotation2209511
version: 1
top level: BBa_K773003
location: https://synbiohub.org/public/igem/BBa_K773003/annotation2209511/range2209511/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_K773003/annotation2209511/range2209511/1
type: Range
persistent identity: range2209511
display id: range2209511
version: 1
top level: BBa_K773003
direction: direction/forward
start: 730
end: 759
orientation: inline

Stop
type: SequenceAnnotation
persistent identity: annotation2209512
display id: annotation2209512
version: 1
top level: BBa_K773003
location: https://synbiohub.org/public/igem/BBa_K773003/annotation2209512/range2209512/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_K773003/annotation2209512/range2209512/1
type: Range
persistent identity: range2209512
display id: range2209512
version: 1
top level: BBa_K773003
direction: direction/forward
start: 760
end: 762
orientation: inline

Stop
type: SequenceAnnotation
persistent identity: annotation2209513
display id: annotation2209513
version: 1
top level: BBa_K773003
location: https://synbiohub.org/public/igem/BBa_K773003/annotation2209513/range2209513/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_K773003/annotation2209513/range2209513/1
type: Range
persistent identity: range2209513
display id: range2209513
version: 1
top level: BBa_K773003
direction: direction/forward
start: 763
end: 765
orientation: inline

BBa_K1151003
type: ComponentDefinition
persistent identity: BBa K1151003
display id: BBa_K1151003
version: 1
was generated by: iGEM to SBOL conversion
description: pnikR
created: 2013-09-20T11:00:00Z
modified: 2015-05-08T01:09:29Z
mutable provenance: This part derives from H. pylori genomic sequence, and it was amplified by PCR.
owned by: james
owned by: myers
mutable description: This promoter derived from Helicobacter pylori, it is costitutively ON and repressed by HpNikR (BBa_K1151000) in nickel presence.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1463_
m user id: 0
owner id: 16632
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: None
star: false
creator: Emanuele Rizzo
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence: https://synbiohub.org/public/igem/BBa_K1151003_sequence/1

BBa_K1151005
type: ComponentDefinition
persistent identity: BBa K1151005
display id: BBa_K1151005
version: 1
was generated by: iGEM to SBOL conversion
description: pUreA
created: 2013-09-20T11:00:00Z
modified: 2015-05-08T01:09:29Z
mutable provenance: This part was synthesized by IDT.
owned by: james
owned by: myers
mutable description: This promoter derived from ureAB operon of Helicobacter pylori. It can be linked by HpNikR (BBa_K1151000) in nickel presence for promoting trascription of the downstream gene
discontinued: false
dominant: false
group u list: _1463_
m user id: 0
owner id: 16632
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: None
star: false
creator: Emanuele Rizzo
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence: https://synbiohub.org/public/igem/BBa_K1151005_sequence/1

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0032
type: ComponentDefinition
persistent identity: BBa B0032
display id: BBa_B0032
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.3 (medium) -- derivative of BBa_0030
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: RBS-3\Weak
sequence annotation: BBa_B0032
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0032_sequence/1

RBS-3\Weak
type: SequenceAnnotation
persistent identity: annotation1709
display id: annotation1709
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
type: Range
persistent identity: range1709
display id: range1709
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation7027
display id: annotation7027
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
type: Range
persistent identity: range7027
display id: range7027
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1710
display id: annotation1710
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
type: Range
persistent identity: range1710
display id: range1710
version: 1
top level: BBa_B0032
direction: direction/forward
start: 7
end: 10
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

GFP
type: ComponentDefinition
persistent identity: BBa E0040
display id: BBa_E0040
version: 1
was generated by: iGEM to SBOL conversion
description: green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
created: 2004-09-29T11:00:00Z
modified: 2016-01-26T02:09:38Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: GFP (mut3b) [note that this part does not have a barcode]
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 4206
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jcbraff
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: GFP protein
sequence: https://synbiohub.org/public/igem/BBa_E0040_sequence/1

GFP protein
type: SequenceAnnotation
persistent identity: annotation1934520
display id: annotation1934520
version: 1
top level: GFP
location: https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
role: feature/protein
role: SO:0000316

https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
type: Range
persistent identity: range1934520
display id: range1934520
version: 1
top level: GFP
direction: direction/forward
start: 1
end: 720
orientation: inline

https://synbiohub.org/public/igem/BBa_K1151029_sequence/1
type: Sequence
persistent identity: BBa K1151029 sequence
display id: BBa_K1151029_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattcaaacgctcttatggccaaccatttaaatccagtttgtattataattgttaattttaaattaattcaatcatactagcatgagcggcttttaacgaactcatgccaaaaaaggatttttctcatactagagtcacacaggaaagtactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagctcattctcctattcttaaagtgtttttccttgaagacataacgaaatcaaggttggatgtaattgtagcaatgttttgatttactaagattaacagaagcgttcattaactaattattatttaaaatgaattagtgttatatttttgaagcgctataaaagcgttattagaagcgcgtttaatacccctttagaatttataaagatcaaaaactcgcaaaaaaaatgagaattaaaattggagtgataatggtactagagaaagaggagaaatactagatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1151008_sequence/1
type: Sequence
persistent identity: BBa K1151008 sequence
display id: BBa_K1151008_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctcattctcctattcttaaagtgtttttccttgaagacataacgaaatcaaggttggatgtaattgtagcaatgttttgatttactaagattaacagaagcgttcattaactaattattatttaaaatgaattagtgttatatttttgaagcgctataaaagcgttattagaagcgcgtttaatacccctttagaatttataaagatcaaaaactcgcaaaaaaaatgagaattaaaattggagtgataatggtactagagaaagaggagaaatactagatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaaggcagcaaacgacgaaaactacgctctagtagcttaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1151009_sequence/1
type: Sequence
persistent identity: BBa K1151009 sequence
display id: BBa_K1151009_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattcaaacgctcttatggccaaccatttaaatccagtttgtattataattgttaattttaaattaattcaatcatactagcatgagcggcttttaacgaactcatgccaaaaaaggatttttctcatactagagtcacacaggaaagtactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0240_sequence/1
type: Sequence
persistent identity: BBa E0240 sequence
display id: BBa_E0240_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaagtactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K773003_sequence/1
type: Sequence
persistent identity: BBa K773003 sequence
display id: BBa_K773003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaaggcagcaaacgacgaaaactacgctctagtagcttaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1151003_sequence/1
type: Sequence
persistent identity: BBa K1151003 sequence
display id: BBa_K1151003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattcaaacgctcttatggccaaccatttaaatccagtttgtattataattgttaattttaaattaattcaatcatactagcatgagcggcttttaacgaactcatgccaaaaaaggatttttctca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1151005_sequence/1
type: Sequence
persistent identity: BBa K1151005 sequence
display id: BBa_K1151005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctcattctcctattcttaaagtgtttttccttgaagacataacgaaatcaaggttggatgtaattgtagcaatgttttgatttactaagattaacagaagcgttcattaactaattattatttaaaatgaattagtgttatatttttgaagcgctataaaagcgttattagaagcgcgtttaatacccctttagaatttataaagatcaaaaactcgcaaaaaaaatgagaattaaaattggagtgataatgg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0032_sequence/1
type: Sequence
persistent identity: BBa B0032 sequence
display id: BBa_B0032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0040_sequence/1
type: Sequence
persistent identity: BBa E0040 sequence
display id: BBa_E0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z