##gff-version 3
##sequence-region BBa_K1151030 1 2166
BBa_K1151030	.	engineered_region	1	1132	.	+	0	ID=BBa_K1151011;Name=BBa_K1151011
BBa_K1151030	.	promoter	1	248	.	+	0	ID=BBa_K1151002;Name=BBa_K1151002;Parent=BBa_K1151011
BBa_K1151030	.	engineered_region	257	1132	.	+	0	ID=BBa_E0240;Name=BBa_E0240;Parent=BBa_K1151011
BBa_K1151030	.	terminator	1004	1083	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0240
BBa_K1151030	.	engineered_region	1004	1083	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1151030	.	stem_loop	1015	1058	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1151030	.	terminator	1092	1132	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0240
BBa_K1151030	.	engineered_region	1092	1132	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1151030	.	polyA_site	1119	1132	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1151030	.	stem_loop	1099	1118	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1151030	.	stop_codon	1125	1125	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1151030	.	ribosome_entry_site	257	269	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_E0240
BBa_K1151030	.	ribosome_entry_site	263	266	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K1151030	.	engineered_region	257	269	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K1151030	.	ribosome_entry_site	257	269	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K1151030	.	CDS	276	995	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_E0240
BBa_K1151030	.	CDS	276	995	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K1151030	.	engineered_region	1141	2166	.	+	0	ID=BBa_K1151008;Name=BBa_K1151008
BBa_K1151030	.	engineered_region	1402	2166	.	+	0	ID=BBa_K773003;Name=BBa_K773003;Parent=BBa_K1151008
BBa_K1151030	.	CDS	1420	2166	.	+	0	ID=annotation2209509;Name=mCherry-LVA;Parent=BBa_K773003
BBa_K1151030	.	start_codon	1420	1422	.	+	0	ID=annotation2209510;Name=Start;Parent=BBa_K773003
BBa_K1151030	.	stop_codon	2164	2166	.	+	0	ID=annotation2209513;Name=Stop;Parent=BBa_K773003
BBa_K1151030	.	tag	2131	2160	.	+	0	ID=annotation2209511;Name=ssrA degradation tag: LVA;Parent=BBa_K773003
BBa_K1151030	.	stop_codon	2161	2163	.	+	0	ID=annotation2209512;Name=Stop;Parent=BBa_K773003
BBa_K1151030	.	ribosome_entry_site	1402	1413	.	+	0	ID=annotation2209508;Name=B0034;Parent=BBa_K773003
BBa_K1151030	.	promoter	1141	1393	.	+	0	ID=BBa_K1151005;Name=BBa_K1151005;Parent=BBa_K1151008
>BBa_K1151030
tgagaaaaatccttttttggcatgagttcgttaaaagccgctcatgctagtatgattgaattaatttaaaattaacaat
tataatacaaactggatttaaatggttggccataagagcgtttgaatttgattgtaattattagcttaatcatcattga
cttgttattattaaaacaatacaatcaacaaaccaacattcttttatcattttggagcatttatgcgaacggatgcttc
ttatagggtgctactagagtcacacaggaaagtactagatgcgtaaaggagaagaacttttcactggagttgtcccaat
tcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacgga
aaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatg
gtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgt
acaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgataccctt
gttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactata
actcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattga
agatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaac
cattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaa
cagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaagg
ctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcacc
ttcgggtgggcctttctgcgtttatatactagagctcattctcctattcttaaagtgtttttccttgaagacataacga
aatcaaggttggatgtaattgtagcaatgttttgatttactaagattaacagaagcgttcattaactaattattattta
aaatgaattagtgttatatttttgaagcgctataaaagcgttattagaagcgcgtttaatacccctttagaatttataa
agatcaaaaactcgcaaaaaaaatgagaattaaaattggagtgataatggtactagagaaagaggagaaatactagatg
gtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtga
acggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgac
caagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcac
cccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacg
gcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaa
cttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggac
ggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacct
acaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgagga
ctacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaaggcagca
aacgacgaaaactacgctctagtagcttaataa