BBa_C0061
1
luxI
autoinducer synthetase for AHL
2003-01-31T12:00:00Z
2015-08-31T04:07:23Z
<em>V. fischeri</em> <genbank>AF170104</genbank>
Released HQ 2013
Synthesizes 3OC<sub>6</sub>HSL, which binds to LuxR.</p> <p>The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the Lux repressor, LuxR. Two molecules of LuxR protein form a complex with two molecules the signalling compound HSL. This complex binds to a palindromic site on the promoter, increasing the rate of transcription.</p>
false
false
_1_
0
24
7
In stock
false
<P> <P>An LVA tail (sequence: AANDENYALVA) was added to increase protein degradation. . <P>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
annotation2213985
1
Help:Barcodes
range2213985
1
619
643
annotation7038
1
BBa_C0061
range7038
1
1
618
annotation1761
1
luxI
range1761
1
1
579
annotation1760
1
LVA
range1760
1
580
611
BBa_K805016
1
BBa_K805016
LuxI
2012-09-24T11:00:00Z
2015-05-08T01:13:25Z
no
Released HQ 2013
test
false
false
_1062_
0
11895
9
In stock
false
test
false
Han Jiapeng
component2251167
1
BBa_C0061
component2251165
1
BBa_B0034
component2251176
1
BBa_B0015
annotation2251167
1
BBa_C0061
range2251167
1
19
636
annotation2251165
1
BBa_B0034
range2251165
1
1
12
annotation2251176
1
BBa_B0015
range2251176
1
670
798
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation4184
1
stem_loop
range4184
1
12
55
annotation7018
1
BBa_B0010
range7018
1
1
80
BBa_B0015
1
BBa_B0015
double terminator (B0010-B0012)
2003-07-16T11:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Double terminator consisting of BBa_B0010 and BBa_B0012
false
true
_1_
0
24
7
In stock
false
true
Reshma Shetty
component1916610
1
BBa_B0010
component1916612
1
BBa_B0012
annotation1916610
1
BBa_B0010
range1916610
1
1
80
annotation1916612
1
BBa_B0012
range1916612
1
89
129
BBa_B0032
1
BBa_B0032
RBS.3 (medium) -- derivative of BBa_0030
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_41_44_48_46_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). <P>
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation7027
1
BBa_B0032
range7027
1
1
13
annotation1709
1
RBS-3\Weak
range1709
1
1
13
annotation1710
1
RBS
range1710
1
7
10
BBa_K1151004
1
BBa_K1151004
pexbB
2013-09-20T11:00:00Z
2015-05-08T01:09:29Z
This part derives from H. pylori genomic sequences, and it was amplified by PCR.
This promoter derived from exbB-exbD-tonB operon of Helicobacter pylori, it is costitutively ON and repressed by HpNikR (BBa_K1151000) in nickel presence.
false
false
_1463_
0
16632
9
Not in stock
false
None
false
Emanuele Rizzo
BBa_K1151005
1
BBa_K1151005
pUreA
2013-09-20T11:00:00Z
2015-05-08T01:09:29Z
This part was synthesized by IDT.
This promoter derived from ureAB operon of Helicobacter pylori. It can be linked by HpNikR (BBa_K1151000) in nickel presence for promoting trascription of the downstream gene
false
false
_1463_
0
16632
9
It's complicated
false
None
false
Emanuele Rizzo
BBa_R0010
1
LacI
promoter (lacI regulated)
2003-01-31T12:00:00Z
2015-05-08T01:14:14Z
The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
Released HQ 2013
Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
false
true
_1_
0
24
7
In stock
false
<P> <P><P> LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
true
annotation1961227
1
start
range1961227
1
173
173
annotation1961223
1
CAP binding site
range1961223
1
89
126
annotation1961222
1
BBa_R0010
range1961222
1
1
200
annotation1961225
1
-10
range1961225
1
161
166
annotation1961226
1
LacI binding site
range1961226
1
166
200
annotation1961224
1
-35
range1961224
1
137
142
annotation1961221
1
end of LacI coding region (inactive)
range1961221
1
1
88
BBa_K1151031
1
BBa_K1151031
Final 1 sensing
2013-09-23T11:00:00Z
2015-05-08T01:09:29Z
None
Fusion of the following parts: K1151012 + K1151006 + K1151010 + K1151008 + B0015.
false
false
_1463_
0
16632
9
Not in stock
false
None
false
Emanuele Rizzo
component2377877
1
BBa_B0015
component2377830
1
BBa_K1151012
component2377824
1
BBa_B0015
component2377861
1
BBa_K1151010
component2377848
1
BBa_K1151006
component2377870
1
BBa_K1151008
annotation2377830
1
BBa_K1151012
range2377830
1
1
1059
annotation2377848
1
BBa_K1151006
range2377848
1
1068
1877
annotation2377877
1
BBa_B0015
range2377877
1
3971
4099
annotation2377861
1
BBa_K1151010
range2377861
1
1886
2928
annotation2377870
1
BBa_K1151008
range2377870
1
2937
3962
annotation2377824
1
BBa_B0015
range2377824
1
931
1059
BBa_K1151000
1
BBa_K1151000
Nickel-responsive pleiotropic regulator (HpNikR)
2013-09-20T11:00:00Z
2015-05-08T01:09:28Z
It was amplified by PCR from the genomic sequence of Helicobacter pylori G27.
The HpNikR protein is a pleiotropic regulator from Helicobacter pylori. In presence of nickel it can acts as an activator or a repressor depending of the specific promoter that contains its operator sites. Each monomer consists in 133aa for a molecular weight of approximately 17.2 kDa. Apo-HpNikR is a tetramer able to bind up to six Ni2+ ions and then a conformational change allows it to activate or repress trascription.
false
false
_1463_
0
16632
9
Not in stock
false
It was inserted in a pET-15b plasmid vector, in order to have a better storage.
false
Emanuele Rizzo
BBa_K1151012
1
BBa_K1151012
LuxI nickel regulated
2013-09-22T11:00:00Z
2015-05-08T01:09:29Z
none
This is a system, driven by the strong promoter pureA (BBa_K1151005), for the production of LuxI (BBa_C0061) by the
addiction of nickel ions in medium and in presence of HpNikR (BBa_K1151000) as trascription factor.
false
false
_1463_
0
16631
9
It's complicated
false
none
false
Gian Piero Lazzari
component2359203
1
BBa_K805016
component2359189
1
BBa_K1151005
annotation2359203
1
BBa_K805016
range2359203
1
262
1059
annotation2359189
1
BBa_K1151005
range2359189
1
1
253
BBa_K1151008
1
BBa_K1151008
RFP nickel regulated
2013-09-22T11:00:00Z
2015-05-08T01:09:29Z
pUreA was synthesized starting from the genome sequence of H. pylori, instead the other parts coming from iGEM kit.
This part was built as a ligation product between the promoter of our design, pUreA, RBS (B0034), RFP (LVA tag) and the terminator B0015.
false
false
_1463_
0
16631
9
It's complicated
false
None
false
Gian Piero Lazzari
component2360853
1
BBa_K1151005
component2360860
1
BBa_K773003
annotation2360860
1
BBa_K773003
range2360860
1
262
1026
annotation2360853
1
BBa_K1151005
range2360853
1
1
253
BBa_K773003
1
BBa_K773003
mCherry-LVA
2012-09-30T11:00:00Z
2015-05-08T01:13:15Z
This part was modified from a plasmid provided by the Murray lab at Caltech.
Released HQ 2013
This part contains the fluorescent protein mCherry attached to a degradation tag. It will decrease the amount of fluorescent protein over time.
false
false
_1025_
0
11646
9
In stock
false
We modified the AAV sequence to LVA with PCR.
false
Katie Knister
annotation2209512
1
Stop
range2209512
1
760
762
annotation2209510
1
Start
range2209510
1
19
21
annotation2209509
1
mCherry-LVA
range2209509
1
19
765
annotation2209513
1
Stop
range2209513
1
763
765
annotation2209508
1
B0034
range2209508
1
1
12
annotation2209511
1
ssrA degradation tag: LVA
range2209511
1
730
759
BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_K1151006
1
BBa_K1151006
HpNikR coding device
2013-09-20T11:00:00Z
2015-05-08T01:09:29Z
None
It's a simple coding device of HpNikR. The protein is synthesized when IPTG is present.
false
false
_1463_
0
16632
9
It's complicated
false
None.
false
Emanuele Rizzo
component2357433
1
BBa_J04500
component2357441
1
BBa_B0015
component2357434
1
BBa_K1151000
annotation2357434
1
BBa_K1151000
range2357434
1
227
673
annotation2357441
1
BBa_B0015
range2357441
1
682
810
annotation2357433
1
BBa_J04500
range2357433
1
1
220
BBa_K1151010
1
BBa_K1151010
GFP nickel regulated 2
2013-09-22T11:00:00Z
2015-05-08T01:09:29Z
None
This is a nickel sensitive system driven by the promoter pexbB (BBa_K1151004) with a GFP reporter. Normally the production of GFP is switched on, it is possible to repress the GFP expression using nickel ions in medium and in presence of HpNikR (BBa_K1151000) as trascription factor.
false
false
_1463_
0
16631
9
It's complicated
false
None
false
Gian Piero Lazzari
component2373622
1
BBa_E0240
component2373611
1
BBa_K1151004
annotation2373622
1
BBa_E0240
range2373622
1
168
1043
annotation2373611
1
BBa_K1151004
range2373611
1
1
159
BBa_E0040
1
GFP
green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
2004-09-29T11:00:00Z
2016-01-26T02:09:38Z
Released HQ 2013
GFP (mut3b) [note that this part does not have a barcode]
false
true
_11_1_
4206
61
7
In stock
false
true
jcbraff
annotation1934520
1
GFP protein
range1934520
1
1
720
BBa_E0240
1
GFP report
GFP generator
2004-10-17T11:00:00Z
2015-08-31T04:07:26Z
Released HQ 2013
B0032.E0040.B0015
false
true
_11_1_
0
61
7
In stock
false
true
Jennifer Braff
component1249221
1
BBa_B0010
component1249216
1
BBa_E0040
component1249213
1
BBa_B0032
component1249231
1
BBa_B0012
annotation1249231
1
BBa_B0012
range1249231
1
836
876
annotation1249221
1
BBa_B0010
range1249221
1
748
827
annotation1249213
1
BBa_B0032
range1249213
1
1
13
annotation1249216
1
BBa_E0040
range1249216
1
20
739
BBa_J04500
1
BBa_J04500
IPTG inducible promoter with RBS
2005-06-08T11:00:00Z
2015-08-31T04:08:14Z
Davidson Synth-Aces
Released HQ 2013
R0010.B0034
false
true
_16_
0
326
16
In stock
false
false
Kristen DeCelle
component1508149
1
BBa_R0010
component1508159
1
BBa_B0034
annotation1508149
1
BBa_R0010
range1508149
1
1
200
annotation1508159
1
BBa_B0034
range1508159
1
209
220
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1690
1
polya
range1690
1
28
41
annotation1686
1
T7 TE
range1686
1
8
27
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1687
1
stop
range1687
1
34
34
BBa_K1151008_sequence
1
ctcattctcctattcttaaagtgtttttccttgaagacataacgaaatcaaggttggatgtaattgtagcaatgttttgatttactaagattaacagaagcgttcattaactaattattatttaaaatgaattagtgttatatttttgaagcgctataaaagcgttattagaagcgcgtttaatacccctttagaatttataaagatcaaaaactcgcaaaaaaaatgagaattaaaattggagtgataatggtactagagaaagaggagaaatactagatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaaggcagcaaacgacgaaaactacgctctagtagcttaataa
BBa_K773003_sequence
1
aaagaggagaaatactagatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaaggcagcaaacgacgaaaactacgctctagtagcttaataa
BBa_R0010_sequence
1
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
BBa_B0034_sequence
1
aaagaggagaaa
BBa_K1151010_sequence
1
actggatttaaatggttggccataagagcgtttgaatttgattgtaattattagcttaatcatcattgacttgttattattaaaacaatacaatcaacaaaccaacattcttttatcattttggagcatttatgcgaacggatgcttcttatagggtgctactagagtcacacaggaaagtactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_K1151004_sequence
1
actggatttaaatggttggccataagagcgtttgaatttgattgtaattattagcttaatcatcattgacttgttattattaaaacaatacaatcaacaaaccaacattcttttatcattttggagcatttatgcgaacggatgcttcttatagggtgc
BBa_K1151006_sequence
1
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatggatacacccaataaagacgattcaatcatccgcttttcggtttctttacaacaaaatttattagacgaattagacaaccgcatcattaaaaacggctattcttctcgctcagaattagtgcgcgacatgatcagagaaaaattagtagaagacaattgggccgaagataaccctaatgatgagagcaaaatcgccgtgcttgtggtgatttatgatcaccaccaaagggaattgaaccagcgcatgatagacattcagcatgccagcgggacgcatgttttatgcaccactcacattcacatggatgagcataattgtttagagacgattattttacaaggcaattcgtttgaaatccaacgcttgcaactagaaattggggggcttaggggggttaaattcgctaaattgactaaggcgtctagctttgaacacaatgaatagtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_K1151031_sequence
1
ctcattctcctattcttaaagtgtttttccttgaagacataacgaaatcaaggttggatgtaattgtagcaatgttttgatttactaagattaacagaagcgttcattaactaattattatttaaaatgaattagtgttatatttttgaagcgctataaaagcgttattagaagcgcgtttaatacccctttagaatttataaagatcaaaaactcgcaaaaaaaatgagaattaaaattggagtgataatggtactagagaaagaggagaaatactagatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatggatacacccaataaagacgattcaatcatccgcttttcggtttctttacaacaaaatttattagacgaattagacaaccgcatcattaaaaacggctattcttctcgctcagaattagtgcgcgacatgatcagagaaaaattagtagaagacaattgggccgaagataaccctaatgatgagagcaaaatcgccgtgcttgtggtgatttatgatcaccaccaaagggaattgaaccagcgcatgatagacattcagcatgccagcgggacgcatgttttatgcaccactcacattcacatggatgagcataattgtttagagacgattattttacaaggcaattcgtttgaaatccaacgcttgcaactagaaattggggggcttaggggggttaaattcgctaaattgactaaggcgtctagctttgaacacaatgaatagtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagactggatttaaatggttggccataagagcgtttgaatttgattgtaattattagcttaatcatcattgacttgttattattaaaacaatacaatcaacaaaccaacattcttttatcattttggagcatttatgcgaacggatgcttcttatagggtgctactagagtcacacaggaaagtactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagctcattctcctattcttaaagtgtttttccttgaagacataacgaaatcaaggttggatgtaattgtagcaatgttttgatttactaagattaacagaagcgttcattaactaattattatttaaaatgaattagtgttatatttttgaagcgctataaaagcgttattagaagcgcgtttaatacccctttagaatttataaagatcaaaaactcgcaaaaaaaatgagaattaaaattggagtgataatggtactagagaaagaggagaaatactagatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaaggcagcaaacgacgaaaactacgctctagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_C0061_sequence
1
atgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctc
BBa_K1151000_sequence
1
atggatacacccaataaagacgattcaatcatccgcttttcggtttctttacaacaaaatttattagacgaattagacaaccgcatcattaaaaacggctattcttctcgctcagaattagtgcgcgacatgatcagagaaaaattagtagaagacaattgggccgaagataaccctaatgatgagagcaaaatcgccgtgcttgtggtgatttatgatcaccaccaaagggaattgaaccagcgcatgatagacattcagcatgccagcgggacgcatgttttatgcaccactcacattcacatggatgagcataattgtttagagacgattattttacaaggcaattcgtttgaaatccaacgcttgcaactagaaattggggggcttaggggggttaaattcgctaaattgactaaggcgtctagctttgaacacaatgaatag
BBa_K1151012_sequence
1
ctcattctcctattcttaaagtgtttttccttgaagacataacgaaatcaaggttggatgtaattgtagcaatgttttgatttactaagattaacagaagcgttcattaactaattattatttaaaatgaattagtgttatatttttgaagcgctataaaagcgttattagaagcgcgtttaatacccctttagaatttataaagatcaaaaactcgcaaaaaaaatgagaattaaaattggagtgataatggtactagagaaagaggagaaatactagatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_J04500_sequence
1
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaa
BBa_K1151005_sequence
1
ctcattctcctattcttaaagtgtttttccttgaagacataacgaaatcaaggttggatgtaattgtagcaatgttttgatttactaagattaacagaagcgttcattaactaattattatttaaaatgaattagtgttatatttttgaagcgctataaaagcgttattagaagcgcgtttaatacccctttagaatttataaagatcaaaaactcgcaaaaaaaatgagaattaaaattggagtgataatgg
BBa_B0032_sequence
1
tcacacaggaaag
BBa_K805016_sequence
1
aaagaggagaaatactagatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_E0240_sequence
1
tcacacaggaaagtactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_E0040_sequence
1
atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_B0015_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z