BBa_K1151032
type: ComponentDefinition
persistent identity: BBa K1151032
display id: BBa_K1151032
version: 1
was generated by: iGEM to SBOL conversion
description: Final 2 sensing
created: 2013-09-23T11:00:00Z
modified: 2015-05-08T01:09:29Z
mutable provenance: None
owned by: james
owned by: myers
mutable description: Fusion of the following parts: K1151012 + K1151006 + K1151009 + K1151008 + B0015.
discontinued: false
dominant: false
experience: experience/None
group u list: _1463_
m user id: 0
owner id: 16632
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: None
star: false
creator: Emanuele Rizzo
type: DnaRegion
role: SO:0000804
role: partType/Reporter
component: BBa_B0015
component: BBa_K1151006
component: BBa_K1151012
component: BBa_K1151008
component: BBa_K1151009
sequence annotation: BBa_K1151012
sequence annotation: BBa_B0015
sequence annotation: BBa_K1151006
sequence annotation: BBa_K1151009
sequence annotation: BBa_K1151008
sequence: https://synbiohub.org/public/igem/BBa_K1151032_sequence/1

BBa_B0015
type: Component
persistent identity: component2361321
display id: component2361321
version: 1
top level: BBa_K1151032
definition: BBa_B0015
access: public

BBa_K1151006
type: Component
persistent identity: component2361345
display id: component2361345
version: 1
top level: BBa_K1151032
definition: BBa_K1151006
access: public

BBa_K1151012
type: Component
persistent identity: component2361327
display id: component2361327
version: 1
top level: BBa_K1151032
definition: BBa_K1151012
access: public

BBa_K1151008
type: Component
persistent identity: component2361367
display id: component2361367
version: 1
top level: BBa_K1151032
definition: BBa_K1151008
access: public

BBa_K1151009
type: Component
persistent identity: component2361358
display id: component2361358
version: 1
top level: BBa_K1151032
definition: BBa_K1151009
access: public

BBa_B0015
type: Component
persistent identity: component2361374
display id: component2361374
version: 1
top level: BBa_K1151032
definition: BBa_B0015
access: public

BBa_K1151012
type: SequenceAnnotation
persistent identity: annotation2361327
display id: annotation2361327
version: 1
top level: BBa_K1151032
location: https://synbiohub.org/public/igem/BBa_K1151032/annotation2361327/range2361327/1
component: BBa_K1151012

https://synbiohub.org/public/igem/BBa_K1151032/annotation2361327/range2361327/1
type: Range
persistent identity: range2361327
display id: range2361327
version: 1
top level: BBa_K1151032
direction: direction/reverse
start: 1
end: 1059
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2361321
display id: annotation2361321
version: 1
top level: BBa_K1151032
location: https://synbiohub.org/public/igem/BBa_K1151032/annotation2361321/range2361321/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1151032/annotation2361321/range2361321/1
type: Range
persistent identity: range2361321
display id: range2361321
version: 1
top level: BBa_K1151032
direction: direction/reverse
start: 931
end: 1059
orientation: inline

BBa_K1151006
type: SequenceAnnotation
persistent identity: annotation2361345
display id: annotation2361345
version: 1
top level: BBa_K1151032
location: https://synbiohub.org/public/igem/BBa_K1151032/annotation2361345/range2361345/1
component: BBa_K1151006

https://synbiohub.org/public/igem/BBa_K1151032/annotation2361345/range2361345/1
type: Range
persistent identity: range2361345
display id: range2361345
version: 1
top level: BBa_K1151032
direction: direction/reverse
start: 1068
end: 1877
orientation: inline

BBa_K1151009
type: SequenceAnnotation
persistent identity: annotation2361358
display id: annotation2361358
version: 1
top level: BBa_K1151032
location: https://synbiohub.org/public/igem/BBa_K1151032/annotation2361358/range2361358/1
component: BBa_K1151009

https://synbiohub.org/public/igem/BBa_K1151032/annotation2361358/range2361358/1
type: Range
persistent identity: range2361358
display id: range2361358
version: 1
top level: BBa_K1151032
direction: direction/reverse
start: 1886
end: 2896
orientation: inline

BBa_K1151008
type: SequenceAnnotation
persistent identity: annotation2361367
display id: annotation2361367
version: 1
top level: BBa_K1151032
location: https://synbiohub.org/public/igem/BBa_K1151032/annotation2361367/range2361367/1
component: BBa_K1151008

https://synbiohub.org/public/igem/BBa_K1151032/annotation2361367/range2361367/1
type: Range
persistent identity: range2361367
display id: range2361367
version: 1
top level: BBa_K1151032
direction: direction/reverse
start: 2905
end: 3930
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2361374
display id: annotation2361374
version: 1
top level: BBa_K1151032
location: https://synbiohub.org/public/igem/BBa_K1151032/annotation2361374/range2361374/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1151032/annotation2361374/range2361374/1
type: Range
persistent identity: range2361374
display id: range2361374
version: 1
top level: BBa_K1151032
direction: direction/reverse
start: 3939
end: 4067
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_K1151006
type: ComponentDefinition
persistent identity: BBa K1151006
display id: BBa_K1151006
version: 1
was generated by: iGEM to SBOL conversion
description: HpNikR coding device
created: 2013-09-20T11:00:00Z
modified: 2015-05-08T01:09:29Z
mutable provenance: None
owned by: james
owned by: myers
mutable description: It's a simple coding device of HpNikR. The protein is synthesized when IPTG is present.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1463_
m user id: 0
owner id: 16632
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: None.
star: false
creator: Emanuele Rizzo
type: DnaRegion
role: partType/Generator
role: SO:0000804
component: BBa_J04500
component: BBa_B0015
component: BBa_K1151000
sequence annotation: BBa_J04500
sequence annotation: BBa_K1151000
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K1151006_sequence/1

BBa_J04500
type: Component
persistent identity: component2357433
display id: component2357433
version: 1
top level: BBa_K1151006
definition: BBa_J04500
access: public

BBa_B0015
type: Component
persistent identity: component2357441
display id: component2357441
version: 1
top level: BBa_K1151006
definition: BBa_B0015
access: public

BBa_K1151000
type: Component
persistent identity: component2357434
display id: component2357434
version: 1
top level: BBa_K1151006
definition: BBa_K1151000
access: public

BBa_J04500
type: SequenceAnnotation
persistent identity: annotation2357433
display id: annotation2357433
version: 1
top level: BBa_K1151006
location: https://synbiohub.org/public/igem/BBa_K1151006/annotation2357433/range2357433/1
component: BBa_J04500

https://synbiohub.org/public/igem/BBa_K1151006/annotation2357433/range2357433/1
type: Range
persistent identity: range2357433
display id: range2357433
version: 1
top level: BBa_K1151006
direction: direction/reverse
start: 1
end: 220
orientation: inline

BBa_K1151000
type: SequenceAnnotation
persistent identity: annotation2357434
display id: annotation2357434
version: 1
top level: BBa_K1151006
location: https://synbiohub.org/public/igem/BBa_K1151006/annotation2357434/range2357434/1
component: BBa_K1151000

https://synbiohub.org/public/igem/BBa_K1151006/annotation2357434/range2357434/1
type: Range
persistent identity: range2357434
display id: range2357434
version: 1
top level: BBa_K1151006
direction: direction/reverse
start: 227
end: 673
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2357441
display id: annotation2357441
version: 1
top level: BBa_K1151006
location: https://synbiohub.org/public/igem/BBa_K1151006/annotation2357441/range2357441/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1151006/annotation2357441/range2357441/1
type: Range
persistent identity: range2357441
display id: range2357441
version: 1
top level: BBa_K1151006
direction: direction/reverse
start: 682
end: 810
orientation: inline

BBa_K1151008
type: ComponentDefinition
persistent identity: BBa K1151008
display id: BBa_K1151008
version: 1
was generated by: iGEM to SBOL conversion
description: RFP nickel regulated
created: 2013-09-22T11:00:00Z
modified: 2015-05-08T01:09:29Z
mutable provenance: pUreA was synthesized starting from the genome sequence of H. pylori, instead the other parts coming from iGEM kit.
owned by: james
owned by: myers
mutable description: This part was built as a ligation product between the promoter of our design, pUreA, RBS (B0034), RFP (LVA tag) and the terminator B0015.
discontinued: false
dominant: false
experience: experience/None
group u list: _1463_
m user id: 0
owner id: 16631
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: None
star: false
creator: Gian Piero Lazzari
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_K773003
component: BBa_K1151005
sequence annotation: BBa_K1151005
sequence annotation: BBa_K773003
sequence: https://synbiohub.org/public/igem/BBa_K1151008_sequence/1

BBa_K773003
type: Component
persistent identity: component2360860
display id: component2360860
version: 1
top level: BBa_K1151008
definition: BBa_K773003
access: public

BBa_K1151005
type: Component
persistent identity: component2360853
display id: component2360853
version: 1
top level: BBa_K1151008
definition: BBa_K1151005
access: public

BBa_K1151005
type: SequenceAnnotation
persistent identity: annotation2360853
display id: annotation2360853
version: 1
top level: BBa_K1151008
location: https://synbiohub.org/public/igem/BBa_K1151008/annotation2360853/range2360853/1
component: BBa_K1151005

https://synbiohub.org/public/igem/BBa_K1151008/annotation2360853/range2360853/1
type: Range
persistent identity: range2360853
display id: range2360853
version: 1
top level: BBa_K1151008
direction: direction/reverse
start: 1
end: 253
orientation: inline

BBa_K773003
type: SequenceAnnotation
persistent identity: annotation2360860
display id: annotation2360860
version: 1
top level: BBa_K1151008
location: https://synbiohub.org/public/igem/BBa_K1151008/annotation2360860/range2360860/1
component: BBa_K773003

https://synbiohub.org/public/igem/BBa_K1151008/annotation2360860/range2360860/1
type: Range
persistent identity: range2360860
display id: range2360860
version: 1
top level: BBa_K1151008
direction: direction/reverse
start: 262
end: 1026
orientation: inline

BBa_K1151009
type: ComponentDefinition
persistent identity: BBa K1151009
display id: BBa_K1151009
version: 1
was generated by: iGEM to SBOL conversion
description: GFP nickel regulated 1
created: 2013-09-22T11:00:00Z
modified: 2015-05-08T01:09:29Z
mutable provenance: None
owned by: james
owned by: myers
mutable description: This is a nickel sensitive system driven by the promoter pnikR (BBa_K1151003) with a GFP reporter. Normally the production of GFP is switched on, it is possible to repress the GFP expression using nickel ions in medium and in presence of HpNikR (BBa_K1151000) as trascription factor.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1463_
m user id: 0
owner id: 16631
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: None
star: false
creator: Gian Piero Lazzari
type: DnaRegion
role: SO:0000804
role: partType/Reporter
component: BBa_K1151003
component: BBa_E0240
sequence annotation: BBa_K1151003
sequence annotation: BBa_E0240
sequence: https://synbiohub.org/public/igem/BBa_K1151009_sequence/1

BBa_K1151003
type: Component
persistent identity: component2358764
display id: component2358764
version: 1
top level: BBa_K1151009
definition: BBa_K1151003
access: public

BBa_E0240
type: Component
persistent identity: component2358775
display id: component2358775
version: 1
top level: BBa_K1151009
definition: GFP report
access: public

BBa_K1151003
type: SequenceAnnotation
persistent identity: annotation2358764
display id: annotation2358764
version: 1
top level: BBa_K1151009
location: https://synbiohub.org/public/igem/BBa_K1151009/annotation2358764/range2358764/1
component: BBa_K1151003

https://synbiohub.org/public/igem/BBa_K1151009/annotation2358764/range2358764/1
type: Range
persistent identity: range2358764
display id: range2358764
version: 1
top level: BBa_K1151009
direction: direction/reverse
start: 1
end: 127
orientation: inline

BBa_E0240
type: SequenceAnnotation
persistent identity: annotation2358775
display id: annotation2358775
version: 1
top level: BBa_K1151009
location: https://synbiohub.org/public/igem/BBa_K1151009/annotation2358775/range2358775/1
component: BBa_E0240

https://synbiohub.org/public/igem/BBa_K1151009/annotation2358775/range2358775/1
type: Range
persistent identity: range2358775
display id: range2358775
version: 1
top level: BBa_K1151009
direction: direction/reverse
start: 136
end: 1011
orientation: inline

BBa_K1151012
type: ComponentDefinition
persistent identity: BBa K1151012
display id: BBa_K1151012
version: 1
was generated by: iGEM to SBOL conversion
description: LuxI nickel regulated
created: 2013-09-22T11:00:00Z
modified: 2015-05-08T01:09:29Z
mutable provenance: none
owned by: james
owned by: myers
mutable description: This is a system, driven by the strong promoter pureA (BBa_K1151005), for the production of LuxI (BBa_C0061) by the addiction of nickel ions in medium and in presence of HpNikR (BBa_K1151000) as trascription factor.
discontinued: false
dominant: false
group u list: _1463_
m user id: 0
owner id: 16631
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: none
star: false
creator: Gian Piero Lazzari
type: DnaRegion
role: SO:0000804
role: partType/Signalling
component: BBa_K805016
component: BBa_K1151005
sequence annotation: BBa_K1151005
sequence annotation: BBa_K805016
sequence: https://synbiohub.org/public/igem/BBa_K1151012_sequence/1

BBa_K805016
type: Component
persistent identity: component2359203
display id: component2359203
version: 1
top level: BBa_K1151012
definition: BBa_K805016
access: public

BBa_K1151005
type: Component
persistent identity: component2359189
display id: component2359189
version: 1
top level: BBa_K1151012
definition: BBa_K1151005
access: public

BBa_K1151005
type: SequenceAnnotation
persistent identity: annotation2359189
display id: annotation2359189
version: 1
top level: BBa_K1151012
location: https://synbiohub.org/public/igem/BBa_K1151012/annotation2359189/range2359189/1
component: BBa_K1151005

https://synbiohub.org/public/igem/BBa_K1151012/annotation2359189/range2359189/1
type: Range
persistent identity: range2359189
display id: range2359189
version: 1
top level: BBa_K1151012
direction: direction/reverse
start: 1
end: 253
orientation: inline

BBa_K805016
type: SequenceAnnotation
persistent identity: annotation2359203
display id: annotation2359203
version: 1
top level: BBa_K1151012
location: https://synbiohub.org/public/igem/BBa_K1151012/annotation2359203/range2359203/1
component: BBa_K805016

https://synbiohub.org/public/igem/BBa_K1151012/annotation2359203/range2359203/1
type: Range
persistent identity: range2359203
display id: range2359203
version: 1
top level: BBa_K1151012
direction: direction/reverse
start: 262
end: 1059
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

BBa_K1151000
type: ComponentDefinition
persistent identity: BBa K1151000
display id: BBa_K1151000
version: 1
was generated by: iGEM to SBOL conversion
description: Nickel-responsive pleiotropic regulator (HpNikR)
created: 2013-09-20T11:00:00Z
modified: 2015-05-08T01:09:28Z
mutable provenance: It was amplified by PCR from the genomic sequence of Helicobacter pylori G27.
owned by: james
owned by: myers
mutable description: The HpNikR protein is a pleiotropic regulator from Helicobacter pylori. In presence of nickel it can acts as an activator or a repressor depending of the specific promoter that contains its operator sites. Each monomer consists in 133aa for a molecular weight of approximately 17.2 kDa. Apo-HpNikR is a tetramer able to bind up to six Ni2+ ions and then a conformational change allows it to activate or repress trascription.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1463_
m user id: 0
owner id: 16632
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: It was inserted in a pET-15b plasmid vector, in order to have a better storage.
star: false
creator: Emanuele Rizzo
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1151000_sequence/1

BBa_K1151005
type: ComponentDefinition
persistent identity: BBa K1151005
display id: BBa_K1151005
version: 1
was generated by: iGEM to SBOL conversion
description: pUreA
created: 2013-09-20T11:00:00Z
modified: 2015-05-08T01:09:29Z
mutable provenance: This part was synthesized by IDT.
owned by: james
owned by: myers
mutable description: This promoter derived from ureAB operon of Helicobacter pylori. It can be linked by HpNikR (BBa_K1151000) in nickel presence for promoting trascription of the downstream gene
discontinued: false
dominant: false
group u list: _1463_
m user id: 0
owner id: 16632
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: None
star: false
creator: Emanuele Rizzo
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence: https://synbiohub.org/public/igem/BBa_K1151005_sequence/1

BBa_J04500
type: ComponentDefinition
persistent identity: BBa J04500
display id: BBa_J04500
version: 1
was generated by: iGEM to SBOL conversion
description: IPTG inducible promoter with RBS
created: 2005-06-08T11:00:00Z
modified: 2015-08-31T04:08:14Z
mutable provenance: Davidson Synth-Aces
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: R0010.B0034
discontinued: false
dominant: true
experience: experience/Works
group u list: _16_
m user id: 0
owner id: 326
owning group id: 16
sample status: In stock
status: status/Available
bookmark: false
star: false
creator: Kristen DeCelle
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_B0034
component: BBa_R0010
sequence annotation: BBa_R0010
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_J04500_sequence/1

BBa_B0034
type: Component
persistent identity: component1508159
display id: component1508159
version: 1
top level: BBa_J04500
definition: BBa_B0034
access: public

BBa_R0010
type: Component
persistent identity: component1508149
display id: component1508149
version: 1
top level: BBa_J04500
definition: LacI
access: public

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1508149
display id: annotation1508149
version: 1
top level: BBa_J04500
location: https://synbiohub.org/public/igem/BBa_J04500/annotation1508149/range1508149/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_J04500/annotation1508149/range1508149/1
type: Range
persistent identity: range1508149
display id: range1508149
version: 1
top level: BBa_J04500
direction: direction/forward
start: 1
end: 200
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1508159
display id: annotation1508159
version: 1
top level: BBa_J04500
location: https://synbiohub.org/public/igem/BBa_J04500/annotation1508159/range1508159/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J04500/annotation1508159/range1508159/1
type: Range
persistent identity: range1508159
display id: range1508159
version: 1
top level: BBa_J04500
direction: direction/forward
start: 209
end: 220
orientation: inline

GFP report
type: ComponentDefinition
persistent identity: BBa E0240
display id: BBa_E0240
version: 1
was generated by: iGEM to SBOL conversion
description: GFP generator
created: 2004-10-17T11:00:00Z
modified: 2015-08-31T04:07:26Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: B0032.E0040.B0015
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Jennifer Braff
type: DnaRegion
role: SO:0000804
role: partType/Reporter
component: BBa_B0032
component: BBa_B0010
component: BBa_E0040
component: BBa_B0012
sequence annotation: BBa_B0032
sequence annotation: BBa_E0040
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_E0240_sequence/1

BBa_B0032
type: Component
persistent identity: component1249213
display id: component1249213
version: 1
top level: GFP report
definition: BBa_B0032
access: public

BBa_B0010
type: Component
persistent identity: component1249221
display id: component1249221
version: 1
top level: GFP report
definition: BBa_B0010
access: public

BBa_E0040
type: Component
persistent identity: component1249216
display id: component1249216
version: 1
top level: GFP report
definition: GFP
access: public

BBa_B0012
type: Component
persistent identity: component1249231
display id: component1249231
version: 1
top level: GFP report
definition: BBa_B0012
access: public

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1249213
display id: annotation1249213
version: 1
top level: GFP report
location: https://synbiohub.org/public/igem/BBa_E0240/annotation1249213/range1249213/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_E0240/annotation1249213/range1249213/1
type: Range
persistent identity: range1249213
display id: range1249213
version: 1
top level: GFP report
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1249216
display id: annotation1249216
version: 1
top level: GFP report
location: https://synbiohub.org/public/igem/BBa_E0240/annotation1249216/range1249216/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_E0240/annotation1249216/range1249216/1
type: Range
persistent identity: range1249216
display id: range1249216
version: 1
top level: GFP report
direction: direction/forward
start: 20
end: 739
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1249221
display id: annotation1249221
version: 1
top level: GFP report
location: https://synbiohub.org/public/igem/BBa_E0240/annotation1249221/range1249221/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_E0240/annotation1249221/range1249221/1
type: Range
persistent identity: range1249221
display id: range1249221
version: 1
top level: GFP report
direction: direction/forward
start: 748
end: 827
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1249231
display id: annotation1249231
version: 1
top level: GFP report
location: https://synbiohub.org/public/igem/BBa_E0240/annotation1249231/range1249231/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_E0240/annotation1249231/range1249231/1
type: Range
persistent identity: range1249231
display id: range1249231
version: 1
top level: GFP report
direction: direction/forward
start: 836
end: 876
orientation: inline

BBa_K773003
type: ComponentDefinition
persistent identity: BBa K773003
display id: BBa_K773003
version: 1
was generated by: iGEM to SBOL conversion
description: mCherry-LVA
created: 2012-09-30T11:00:00Z
modified: 2015-05-08T01:13:15Z
mutable provenance: This part was modified from a plasmid provided by the Murray lab at Caltech.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: This part contains the fluorescent protein mCherry attached to a degradation tag. It will decrease the amount of fluorescent protein over time.
discontinued: false
dominant: false
group u list: _1025_
m user id: 0
owner id: 11646
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: We modified the AAV sequence to LVA with PCR.
star: false
creator: Katie Knister
type: DnaRegion
role: SO:0000804
role: partType/Translational Unit
sequence annotation: B0034
sequence annotation: mCherry-LVA
sequence annotation: Start
sequence annotation: ssrA degradation tag: LVA
sequence annotation: Stop
sequence: https://synbiohub.org/public/igem/BBa_K773003_sequence/1

B0034
type: SequenceAnnotation
persistent identity: annotation2209508
display id: annotation2209508
version: 1
top level: BBa_K773003
location: https://synbiohub.org/public/igem/BBa_K773003/annotation2209508/range2209508/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_K773003/annotation2209508/range2209508/1
type: Range
persistent identity: range2209508
display id: range2209508
version: 1
top level: BBa_K773003
direction: direction/forward
start: 1
end: 12
orientation: inline

mCherry-LVA
type: SequenceAnnotation
persistent identity: annotation2209509
display id: annotation2209509
version: 1
top level: BBa_K773003
location: https://synbiohub.org/public/igem/BBa_K773003/annotation2209509/range2209509/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K773003/annotation2209509/range2209509/1
type: Range
persistent identity: range2209509
display id: range2209509
version: 1
top level: BBa_K773003
direction: direction/forward
start: 19
end: 765
orientation: inline

Start
type: SequenceAnnotation
persistent identity: annotation2209510
display id: annotation2209510
version: 1
top level: BBa_K773003
location: https://synbiohub.org/public/igem/BBa_K773003/annotation2209510/range2209510/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_K773003/annotation2209510/range2209510/1
type: Range
persistent identity: range2209510
display id: range2209510
version: 1
top level: BBa_K773003
direction: direction/forward
start: 19
end: 21
orientation: inline

ssrA degradation tag: LVA
type: SequenceAnnotation
persistent identity: annotation2209511
display id: annotation2209511
version: 1
top level: BBa_K773003
location: https://synbiohub.org/public/igem/BBa_K773003/annotation2209511/range2209511/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_K773003/annotation2209511/range2209511/1
type: Range
persistent identity: range2209511
display id: range2209511
version: 1
top level: BBa_K773003
direction: direction/forward
start: 730
end: 759
orientation: inline

Stop
type: SequenceAnnotation
persistent identity: annotation2209512
display id: annotation2209512
version: 1
top level: BBa_K773003
location: https://synbiohub.org/public/igem/BBa_K773003/annotation2209512/range2209512/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K773003/annotation2209512/range2209512/1
type: Range
persistent identity: range2209512
display id: range2209512
version: 1
top level: BBa_K773003
direction: direction/forward
start: 760
end: 762
orientation: inline

Stop
type: SequenceAnnotation
persistent identity: annotation2209513
display id: annotation2209513
version: 1
top level: BBa_K773003
location: https://synbiohub.org/public/igem/BBa_K773003/annotation2209513/range2209513/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_K773003/annotation2209513/range2209513/1
type: Range
persistent identity: range2209513
display id: range2209513
version: 1
top level: BBa_K773003
direction: direction/forward
start: 763
end: 765
orientation: inline

BBa_K1151003
type: ComponentDefinition
persistent identity: BBa K1151003
display id: BBa_K1151003
version: 1
was generated by: iGEM to SBOL conversion
description: pnikR
created: 2013-09-20T11:00:00Z
modified: 2015-05-08T01:09:29Z
mutable provenance: This part derives from H. pylori genomic sequence, and it was amplified by PCR.
owned by: james
owned by: myers
mutable description: This promoter derived from Helicobacter pylori, it is costitutively ON and repressed by HpNikR (BBa_K1151000) in nickel presence.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1463_
m user id: 0
owner id: 16632
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: None
star: false
creator: Emanuele Rizzo
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence: https://synbiohub.org/public/igem/BBa_K1151003_sequence/1

BBa_K805016
type: ComponentDefinition
persistent identity: BBa K805016
display id: BBa_K805016
version: 1
was generated by: iGEM to SBOL conversion
description: LuxI
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:25Z
mutable provenance: no
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: test
discontinued: false
dominant: false
experience: experience/None
group u list: _1062_
m user id: 0
owner id: 11895
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: test
star: false
creator: Han Jiapeng
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0015
component: BBa_C0061
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_C0061
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K805016_sequence/1

BBa_B0015
type: Component
persistent identity: component2251176
display id: component2251176
version: 1
top level: BBa_K805016
definition: BBa_B0015
access: public

BBa_C0061
type: Component
persistent identity: component2251167
display id: component2251167
version: 1
top level: BBa_K805016
definition: luxI
access: public

BBa_B0034
type: Component
persistent identity: component2251165
display id: component2251165
version: 1
top level: BBa_K805016
definition: BBa_B0034
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2251165
display id: annotation2251165
version: 1
top level: BBa_K805016
location: https://synbiohub.org/public/igem/BBa_K805016/annotation2251165/range2251165/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K805016/annotation2251165/range2251165/1
type: Range
persistent identity: range2251165
display id: range2251165
version: 1
top level: BBa_K805016
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_C0061
type: SequenceAnnotation
persistent identity: annotation2251167
display id: annotation2251167
version: 1
top level: BBa_K805016
location: https://synbiohub.org/public/igem/BBa_K805016/annotation2251167/range2251167/1
component: BBa_C0061

https://synbiohub.org/public/igem/BBa_K805016/annotation2251167/range2251167/1
type: Range
persistent identity: range2251167
display id: range2251167
version: 1
top level: BBa_K805016
direction: direction/forward
start: 19
end: 636
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2251176
display id: annotation2251176
version: 1
top level: BBa_K805016
location: https://synbiohub.org/public/igem/BBa_K805016/annotation2251176/range2251176/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K805016/annotation2251176/range2251176/1
type: Range
persistent identity: range2251176
display id: range2251176
version: 1
top level: BBa_K805016
direction: direction/reverse
start: 670
end: 798
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

LacI
type: ComponentDefinition
persistent identity: BBa R0010
display id: BBa_R0010
version: 1
was generated by: iGEM to SBOL conversion
description: promoter (lacI regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
star: true
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: end of LacI coding region (inactive)
sequence annotation: BBa_R0010
sequence annotation: CAP binding site
sequence annotation: -35
sequence annotation: -10
sequence annotation: LacI binding site
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_R0010_sequence/1

end of LacI coding region (inactive)
type: SequenceAnnotation
persistent identity: annotation1961221
display id: annotation1961221
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
type: Range
persistent identity: range1961221
display id: range1961221
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 88
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1961222
display id: annotation1961222
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
type: Range
persistent identity: range1961222
display id: range1961222
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 200
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation1961223
display id: annotation1961223
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
type: Range
persistent identity: range1961223
display id: range1961223
version: 1
top level: LacI
direction: direction/forward
start: 89
end: 126
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1961224
display id: annotation1961224
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
type: Range
persistent identity: range1961224
display id: range1961224
version: 1
top level: LacI
direction: direction/forward
start: 137
end: 142
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1961225
display id: annotation1961225
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
type: Range
persistent identity: range1961225
display id: range1961225
version: 1
top level: LacI
direction: direction/forward
start: 161
end: 166
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation1961226
display id: annotation1961226
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
type: Range
persistent identity: range1961226
display id: range1961226
version: 1
top level: LacI
direction: direction/forward
start: 166
end: 200
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1961227
display id: annotation1961227
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
type: Range
persistent identity: range1961227
display id: range1961227
version: 1
top level: LacI
direction: direction/forward
start: 173
end: 173
orientation: inline

BBa_B0032
type: ComponentDefinition
persistent identity: BBa B0032
display id: BBa_B0032
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.3 (medium) -- derivative of BBa_0030
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: RBS-3\Weak
sequence annotation: BBa_B0032
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0032_sequence/1

RBS-3\Weak
type: SequenceAnnotation
persistent identity: annotation1709
display id: annotation1709
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
type: Range
persistent identity: range1709
display id: range1709
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation7027
display id: annotation7027
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
type: Range
persistent identity: range7027
display id: range7027
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1710
display id: annotation1710
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
type: Range
persistent identity: range1710
display id: range1710
version: 1
top level: BBa_B0032
direction: direction/forward
start: 7
end: 10
orientation: inline

GFP
type: ComponentDefinition
persistent identity: BBa E0040
display id: BBa_E0040
version: 1
was generated by: iGEM to SBOL conversion
description: green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
created: 2004-09-29T11:00:00Z
modified: 2016-01-26T02:09:38Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: GFP (mut3b) [note that this part does not have a barcode]
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 4206
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jcbraff
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: GFP protein
sequence: https://synbiohub.org/public/igem/BBa_E0040_sequence/1

GFP protein
type: SequenceAnnotation
persistent identity: annotation1934520
display id: annotation1934520
version: 1
top level: GFP
location: https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
type: Range
persistent identity: range1934520
display id: range1934520
version: 1
top level: GFP
direction: direction/forward
start: 1
end: 720
orientation: inline

luxI
type: ComponentDefinition
persistent identity: BBa C0061
display id: BBa_C0061
version: 1
was generated by: iGEM to SBOL conversion
description: autoinducer synthetase for AHL
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: V. fischeri <genbank>AF170104</genbank>
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Synthesizes 3OC6HSL, which binds to LuxR. The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the Lux repressor, LuxR. Two molecules of LuxR protein form a complex with two molecules the signalling compound HSL. This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: An LVA tail (sequence: AANDENYALVA) was added to increase protein degradation. .
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: BBa_C0061
sequence annotation: luxI
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0061_sequence/1

BBa_C0061
type: SequenceAnnotation
persistent identity: annotation7038
display id: annotation7038
version: 1
top level: luxI
location: https://synbiohub.org/public/igem/BBa_C0061/annotation7038/range7038/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_C0061/annotation7038/range7038/1
type: Range
persistent identity: range7038
display id: range7038
version: 1
top level: luxI
direction: direction/forward
start: 1
end: 618
orientation: inline

luxI
type: SequenceAnnotation
persistent identity: annotation1761
display id: annotation1761
version: 1
top level: luxI
location: https://synbiohub.org/public/igem/BBa_C0061/annotation1761/range1761/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_C0061/annotation1761/range1761/1
type: Range
persistent identity: range1761
display id: range1761
version: 1
top level: luxI
direction: direction/forward
start: 1
end: 579
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation1760
display id: annotation1760
version: 1
top level: luxI
location: https://synbiohub.org/public/igem/BBa_C0061/annotation1760/range1760/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_C0061/annotation1760/range1760/1
type: Range
persistent identity: range1760
display id: range1760
version: 1
top level: luxI
direction: direction/forward
start: 580
end: 611
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213985
display id: annotation2213985
version: 1
top level: luxI
location: https://synbiohub.org/public/igem/BBa_C0061/annotation2213985/range2213985/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_C0061/annotation2213985/range2213985/1
type: Range
persistent identity: range2213985
display id: range2213985
version: 1
top level: luxI
direction: direction/forward
start: 619
end: 643
orientation: inline

https://synbiohub.org/public/igem/BBa_K1151032_sequence/1
type: Sequence
persistent identity: BBa K1151032 sequence
display id: BBa_K1151032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctcattctcctattcttaaagtgtttttccttgaagacataacgaaatcaaggttggatgtaattgtagcaatgttttgatttactaagattaacagaagcgttcattaactaattattatttaaaatgaattagtgttatatttttgaagcgctataaaagcgttattagaagcgcgtttaatacccctttagaatttataaagatcaaaaactcgcaaaaaaaatgagaattaaaattggagtgataatggtactagagaaagaggagaaatactagatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatggatacacccaataaagacgattcaatcatccgcttttcggtttctttacaacaaaatttattagacgaattagacaaccgcatcattaaaaacggctattcttctcgctcagaattagtgcgcgacatgatcagagaaaaattagtagaagacaattgggccgaagataaccctaatgatgagagcaaaatcgccgtgcttgtggtgatttatgatcaccaccaaagggaattgaaccagcgcatgatagacattcagcatgccagcgggacgcatgttttatgcaccactcacattcacatggatgagcataattgtttagagacgattattttacaaggcaattcgtttgaaatccaacgcttgcaactagaaattggggggcttaggggggttaaattcgctaaattgactaaggcgtctagctttgaacacaatgaatagtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagaattcaaacgctcttatggccaaccatttaaatccagtttgtattataattgttaattttaaattaattcaatcatactagcatgagcggcttttaacgaactcatgccaaaaaa…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1151006_sequence/1
type: Sequence
persistent identity: BBa K1151006 sequence
display id: BBa_K1151006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatggatacacccaataaagacgattcaatcatccgcttttcggtttctttacaacaaaatttattagacgaattagacaaccgcatcattaaaaacggctattcttctcgctcagaattagtgcgcgacatgatcagagaaaaattagtagaagacaattgggccgaagataaccctaatgatgagagcaaaatcgccgtgcttgtggtgatttatgatcaccaccaaagggaattgaaccagcgcatgatagacattcagcatgccagcgggacgcatgttttatgcaccactcacattcacatggatgagcataattgtttagagacgattattttacaaggcaattcgtttgaaatccaacgcttgcaactagaaattggggggcttaggggggttaaattcgctaaattgactaaggcgtctagctttgaacacaatgaatagtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1151008_sequence/1
type: Sequence
persistent identity: BBa K1151008 sequence
display id: BBa_K1151008_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctcattctcctattcttaaagtgtttttccttgaagacataacgaaatcaaggttggatgtaattgtagcaatgttttgatttactaagattaacagaagcgttcattaactaattattatttaaaatgaattagtgttatatttttgaagcgctataaaagcgttattagaagcgcgtttaatacccctttagaatttataaagatcaaaaactcgcaaaaaaaatgagaattaaaattggagtgataatggtactagagaaagaggagaaatactagatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaaggcagcaaacgacgaaaactacgctctagtagcttaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1151009_sequence/1
type: Sequence
persistent identity: BBa K1151009 sequence
display id: BBa_K1151009_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattcaaacgctcttatggccaaccatttaaatccagtttgtattataattgttaattttaaattaattcaatcatactagcatgagcggcttttaacgaactcatgccaaaaaaggatttttctcatactagagtcacacaggaaagtactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1151012_sequence/1
type: Sequence
persistent identity: BBa K1151012 sequence
display id: BBa_K1151012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctcattctcctattcttaaagtgtttttccttgaagacataacgaaatcaaggttggatgtaattgtagcaatgttttgatttactaagattaacagaagcgttcattaactaattattatttaaaatgaattagtgttatatttttgaagcgctataaaagcgttattagaagcgcgtttaatacccctttagaatttataaagatcaaaaactcgcaaaaaaaatgagaattaaaattggagtgataatggtactagagaaagaggagaaatactagatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1151000_sequence/1
type: Sequence
persistent identity: BBa K1151000 sequence
display id: BBa_K1151000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggatacacccaataaagacgattcaatcatccgcttttcggtttctttacaacaaaatttattagacgaattagacaaccgcatcattaaaaacggctattcttctcgctcagaattagtgcgcgacatgatcagagaaaaattagtagaagacaattgggccgaagataaccctaatgatgagagcaaaatcgccgtgcttgtggtgatttatgatcaccaccaaagggaattgaaccagcgcatgatagacattcagcatgccagcgggacgcatgttttatgcaccactcacattcacatggatgagcataattgtttagagacgattattttacaaggcaattcgtttgaaatccaacgcttgcaactagaaattggggggcttaggggggttaaattcgctaaattgactaaggcgtctagctttgaacacaatgaatag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1151005_sequence/1
type: Sequence
persistent identity: BBa K1151005 sequence
display id: BBa_K1151005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctcattctcctattcttaaagtgtttttccttgaagacataacgaaatcaaggttggatgtaattgtagcaatgttttgatttactaagattaacagaagcgttcattaactaattattatttaaaatgaattagtgttatatttttgaagcgctataaaagcgttattagaagcgcgtttaatacccctttagaatttataaagatcaaaaactcgcaaaaaaaatgagaattaaaattggagtgataatgg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0240_sequence/1
type: Sequence
persistent identity: BBa E0240 sequence
display id: BBa_E0240_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaagtactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J04500_sequence/1
type: Sequence
persistent identity: BBa J04500 sequence
display id: BBa_J04500_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K773003_sequence/1
type: Sequence
persistent identity: BBa K773003 sequence
display id: BBa_K773003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaaggcagcaaacgacgaaaactacgctctagtagcttaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1151003_sequence/1
type: Sequence
persistent identity: BBa K1151003 sequence
display id: BBa_K1151003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattcaaacgctcttatggccaaccatttaaatccagtttgtattataattgttaattttaaattaattcaatcatactagcatgagcggcttttaacgaactcatgccaaaaaaggatttttctca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K805016_sequence/1
type: Sequence
persistent identity: BBa K805016 sequence
display id: BBa_K805016_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0032_sequence/1
type: Sequence
persistent identity: BBa B0032 sequence
display id: BBa_B0032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0010_sequence/1
type: Sequence
persistent identity: BBa R0010 sequence
display id: BBa_R0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0040_sequence/1
type: Sequence
persistent identity: BBa E0040 sequence
display id: BBa_E0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: myers
owned by: james
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0061_sequence/1
type: Sequence
persistent identity: BBa C0061 sequence
display id: BBa_C0061_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z