##gff-version 3
##sequence-region BBa_K1151033 1 4188
BBa_K1151033	.	engineered_region	1	1059	.	+	0	ID=BBa_K1151012;Name=BBa_K1151012
BBa_K1151033	.	engineered_region	262	1059	.	+	0	ID=BBa_K805016;Name=BBa_K805016;Parent=BBa_K1151012
BBa_K1151033	.	ribosome_entry_site	262	273	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K805016
BBa_K1151033	.	conserved_region	266	269	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1151033	.	CDS	280	897	.	+	0	ID=BBa_C0061;Name=BBa_C0061;Parent=BBa_K805016
BBa_K1151033	.	engineered_region	280	897	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_K1151033	.	tag	859	890	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_K1151033	.	engineered_tag	898	922	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_K1151033	.	CDS	280	858	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_K1151033	.	terminator	931	1059	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K805016
BBa_K1151033	.	terminator	931	1010	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1151033	.	stem_loop	942	985	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1151033	.	engineered_region	931	1010	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1151033	.	terminator	1019	1059	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1151033	.	engineered_region	1019	1059	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1151033	.	stop_codon	1052	1052	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1151033	.	stem_loop	1026	1045	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1151033	.	polyA_site	1046	1059	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1151033	.	promoter	1	253	.	+	0	ID=BBa_K1151005;Name=BBa_K1151005;Parent=BBa_K1151012
BBa_K1151033	.	terminator	931	1059	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1151033	.	terminator	931	1010	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1151033	.	stem_loop	942	985	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1151033	.	engineered_region	931	1010	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1151033	.	terminator	1019	1059	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1151033	.	engineered_region	1019	1059	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1151033	.	stop_codon	1052	1052	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1151033	.	stem_loop	1026	1045	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1151033	.	polyA_site	1046	1059	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1151033	.	terminator	4060	4188	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1151033	.	terminator	4060	4139	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1151033	.	stem_loop	4071	4114	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1151033	.	engineered_region	4060	4139	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1151033	.	terminator	4148	4188	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1151033	.	engineered_region	4148	4188	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1151033	.	stop_codon	4181	4181	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1151033	.	stem_loop	4155	4174	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1151033	.	polyA_site	4175	4188	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1151033	.	engineered_region	3026	4051	.	+	0	ID=BBa_K1151008;Name=BBa_K1151008
BBa_K1151033	.	promoter	3026	3278	.	+	0	ID=BBa_K1151005;Name=BBa_K1151005;Parent=BBa_K1151008
BBa_K1151033	.	engineered_region	3287	4051	.	+	0	ID=BBa_K773003;Name=BBa_K773003;Parent=BBa_K1151008
BBa_K1151033	.	stop_codon	4049	4051	.	+	0	ID=annotation2209513;Name=Stop;Parent=BBa_K773003
BBa_K1151033	.	start_codon	3305	3307	.	+	0	ID=annotation2209510;Name=Start;Parent=BBa_K773003
BBa_K1151033	.	stop_codon	4046	4048	.	+	0	ID=annotation2209512;Name=Stop;Parent=BBa_K773003
BBa_K1151033	.	CDS	3305	4051	.	+	0	ID=annotation2209509;Name=mCherry-LVA;Parent=BBa_K773003
BBa_K1151033	.	tag	4016	4045	.	+	0	ID=annotation2209511;Name=ssrA degradation tag: LVA;Parent=BBa_K773003
BBa_K1151033	.	ribosome_entry_site	3287	3298	.	+	0	ID=annotation2209508;Name=B0034;Parent=BBa_K773003
BBa_K1151033	.	engineered_region	1886	3017	.	+	0	ID=BBa_K1151011;Name=BBa_K1151011
BBa_K1151033	.	promoter	1886	2133	.	+	0	ID=BBa_K1151002;Name=BBa_K1151002;Parent=BBa_K1151011
BBa_K1151033	.	engineered_region	2142	3017	.	+	0	ID=BBa_E0240;Name=BBa_E0240;Parent=BBa_K1151011
BBa_K1151033	.	CDS	2161	2880	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_E0240
BBa_K1151033	.	CDS	2161	2880	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K1151033	.	terminator	2889	2968	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0240
BBa_K1151033	.	stem_loop	2900	2943	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1151033	.	engineered_region	2889	2968	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1151033	.	ribosome_entry_site	2142	2154	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_E0240
BBa_K1151033	.	ribosome_entry_site	2148	2151	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K1151033	.	engineered_region	2142	2154	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K1151033	.	ribosome_entry_site	2142	2154	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K1151033	.	terminator	2977	3017	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0240
BBa_K1151033	.	engineered_region	2977	3017	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1151033	.	stop_codon	3010	3010	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1151033	.	stem_loop	2984	3003	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1151033	.	polyA_site	3004	3017	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1151033	.	engineered_region	1068	1877	.	+	0	ID=BBa_K1151006;Name=BBa_K1151006
BBa_K1151033	.	engineered_region	1068	1287	.	+	0	ID=BBa_J04500;Name=BBa_J04500;Parent=BBa_K1151006
BBa_K1151033	.	ribosome_entry_site	1276	1287	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J04500
BBa_K1151033	.	conserved_region	1280	1283	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1151033	.	promoter	1068	1267	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_J04500
BBa_K1151033	.	non_covalent_binding_site	1233	1267	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K1151033	.	non_covalent_binding_site	1156	1193	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K1151033	.	engineered_region	1068	1267	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K1151033	.	promoter	1204	1209	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K1151033	.	CDS	1068	1155	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K1151033	.	start_codon	1240	1240	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K1151033	.	promoter	1228	1233	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K1151033	.	terminator	1749	1877	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K1151006
BBa_K1151033	.	terminator	1749	1828	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1151033	.	stem_loop	1760	1803	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1151033	.	engineered_region	1749	1828	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1151033	.	terminator	1837	1877	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1151033	.	engineered_region	1837	1877	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1151033	.	stop_codon	1870	1870	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1151033	.	stem_loop	1844	1863	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1151033	.	polyA_site	1864	1877	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1151033	.	CDS	1294	1740	.	+	0	ID=BBa_K1151000;Name=BBa_K1151000;Parent=BBa_K1151006
>BBa_K1151033
ctcattctcctattcttaaagtgtttttccttgaagacataacgaaatcaaggttggatgtaattgtagcaatgttttg
atttactaagattaacagaagcgttcattaactaattattatttaaaatgaattagtgttatatttttgaagcgctata
aaagcgttattagaagcgcgtttaatacccctttagaatttataaagatcaaaaactcgcaaaaaaaatgagaattaaa
attggagtgataatggtactagagaaagaggagaaatactagatgactataatgataaaaaaatcggattttttggcaa
ttccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgt
agaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgta
agtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacaga
gtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgc
tagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagta
acatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtat
taggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacga
cgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaac
gaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactgg
ctcaccttcgggtgggcctttctgcgtttatatactagagcaatacgcaaaccgcctctccccgcgcgttggccgattc
attaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcac
tcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcac
acatactagagaaagaggagaaatactagatggatacacccaataaagacgattcaatcatccgcttttcggtttcttt
acaacaaaatttattagacgaattagacaaccgcatcattaaaaacggctattcttctcgctcagaattagtgcgcgac
atgatcagagaaaaattagtagaagacaattgggccgaagataaccctaatgatgagagcaaaatcgccgtgcttgtgg
tgatttatgatcaccaccaaagggaattgaaccagcgcatgatagacattcagcatgccagcgggacgcatgttttatg
caccactcacattcacatggatgagcataattgtttagagacgattattttacaaggcaattcgtttgaaatccaacgc
ttgcaactagaaattggggggcttaggggggttaaattcgctaaattgactaaggcgtctagctttgaacacaatgaat
agtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcgg
tgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtgagaaaaatc
cttttttggcatgagttcgttaaaagccgctcatgctagtatgattgaattaatttaaaattaacaattataatacaaa
ctggatttaaatggttggccataagagcgtttgaatttgattgtaattattagcttaatcatcattgacttgttattat
taaaacaatacaatcaacaaaccaacattcttttatcattttggagcatttatgcgaacggatgcttcttatagggtgc
tactagagtcacacaggaaagtactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaat
tagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttaccct
taaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgc
tttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaa
ctatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaat
cgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaat
gtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcg
ttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtc
cacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctggg
attacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaa
gactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggc
ctttctgcgtttatatactagagctcattctcctattcttaaagtgtttttccttgaagacataacgaaatcaaggttg
gatgtaattgtagcaatgttttgatttactaagattaacagaagcgttcattaactaattattatttaaaatgaattag
tgttatatttttgaagcgctataaaagcgttattagaagcgcgtttaatacccctttagaatttataaagatcaaaaac
tcgcaaaaaaaatgagaattaaaattggagtgataatggtactagagaaagaggagaaatactagatggtgagcaaggg
cgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgag
ttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggcc
ccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacat
ccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtg
accgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccg
acggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaa
gggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaag
aagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcg
tggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaaggcagcaaacgacgaaaa
ctacgctctagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcg
ttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
a