LOCUS       BBa_K1151036            1829 bp    DNA     linear       22-Dec-2024
DEFINITION  Double generator NikR-GFP, IPTG-nickel regulated
ACCESSION   BBa_K1151036
VERSION     BBa_K1151036.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    1..220
                     /label=BBa_J04500
     CDS             227..673
                     /label=BBa_K1151000
     stem_loop       693..736
                     /label=stem_loop
     misc_feature    682..761
                     /label=BBa_B0010
     terminator      682..761
                     /label=BBa_B0010
     stem_loop       777..796
                     /label=T7 TE
     misc_feature    803..803
                     /label=stop
     polyA_site      797..810
                     /label=polya
     misc_feature    770..810
                     /label=BBa_B0012
     terminator      770..810
                     /label=BBa_B0012
     terminator      682..810
                     /label=BBa_B0015
     misc_feature    1..810
                     /label=BBa_K1151006
     promoter        819..945
                     /label=BBa_K1151003
     RBS             954..966
                     /label=RBS-3\Weak
     RBS             960..963
                     /label=RBS
     misc_feature    954..966
                     /label=BBa_B0032
     RBS             954..966
                     /label=BBa_B0032
     CDS             973..1692
                     /label=GFP protein
     CDS             973..1692
                     /label=BBa_E0040
     stem_loop       1712..1755
                     /label=stem_loop
     misc_feature    1701..1780
                     /label=BBa_B0010
     terminator      1701..1780
                     /label=BBa_B0010
     stem_loop       1796..1815
                     /label=T7 TE
     misc_feature    1822..1822
                     /label=stop
     polyA_site      1816..1829
                     /label=polya
     misc_feature    1789..1829
                     /label=BBa_B0012
     terminator      1789..1829
                     /label=BBa_B0012
     misc_feature    954..1829
                     /label=BBa_E0240
     misc_feature    819..1829
                     /label=BBa_K1151009
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgg atacacccaa
      241 taaagacgat tcaatcatcc gcttttcggt ttctttacaa caaaatttat tagacgaatt
      301 agacaaccgc atcattaaaa acggctattc ttctcgctca gaattagtgc gcgacatgat
      361 cagagaaaaa ttagtagaag acaattgggc cgaagataac cctaatgatg agagcaaaat
      421 cgccgtgctt gtggtgattt atgatcacca ccaaagggaa ttgaaccagc gcatgataga
      481 cattcagcat gccagcggga cgcatgtttt atgcaccact cacattcaca tggatgagca
      541 taattgttta gagacgatta ttttacaagg caattcgttt gaaatccaac gcttgcaact
      601 agaaattggg gggcttaggg gggttaaatt cgctaaattg actaaggcgt ctagctttga
      661 acacaatgaa tagtactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact
      721 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct
      781 caccttcggg tgggcctttc tgcgtttata tactagagaa ttcaaacgct cttatggcca
      841 accatttaaa tccagtttgt attataattg ttaattttaa attaattcaa tcatactagc
      901 atgagcggct tttaacgaac tcatgccaaa aaaggatttt tctcatacta gagtcacaca
      961 ggaaagtact agatgcgtaa aggagaagaa cttttcactg gagttgtccc aattcttgtt
     1021 gaattagatg gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg tgaaggtgat
     1081 gcaacatacg gaaaacttac ccttaaattt atttgcacta ctggaaaact acctgttcca
     1141 tggccaacac ttgtcactac tttcggttat ggtgttcaat gctttgcgag atacccagat
     1201 catatgaaac agcatgactt tttcaagagt gccatgcccg aaggttatgt acaggaaaga
     1261 actatatttt tcaaagatga cgggaactac aagacacgtg ctgaagtcaa gtttgaaggt
     1321 gatacccttg ttaatagaat cgagttaaaa ggtattgatt ttaaagaaga tggaaacatt
     1381 cttggacaca aattggaata caactataac tcacacaatg tatacatcat ggcagacaaa
     1441 caaaagaatg gaatcaaagt taacttcaaa attagacaca acattgaaga tggaagcgtt
     1501 caactagcag accattatca acaaaatact ccaattggcg atggccctgt ccttttacca
     1561 gacaaccatt acctgtccac acaatctgcc ctttcgaaag atcccaacga aaagagagac
     1621 cacatggtcc ttcttgagtt tgtaacagct gctgggatta cacatggcat ggatgaacta
     1681 tacaaataat aatactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg
     1741 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc
     1801 accttcgggt gggcctttct gcgtttata
//