LOCUS BBa_K1151037 3092 bp DNA linear 27-Dec-2024 DEFINITION HpNikR-LuxR-LuxI-Hpn ACCESSION BBa_K1151037 VERSION BBa_K1151037.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 misc_feature 213..216 /label=conserved RBS 209..220 /label=BBa_B0034 misc_feature 1..220 /label=BBa_J04500 CDS 227..673 /label=BBa_K1151000 stem_loop 693..736 /label=stem_loop misc_feature 682..761 /label=BBa_B0010 terminator 682..761 /label=BBa_B0010 stem_loop 777..796 /label=T7 TE misc_feature 803..803 /label=stop polyA_site 797..810 /label=polya misc_feature 770..810 /label=BBa_B0012 terminator 770..810 /label=BBa_B0012 terminator 682..810 /label=BBa_B0015 misc_feature 1..810 /label=BBa_K1151006 promoter 819..1071 /label=BBa_K1151005 misc_feature 1084..1087 /label=conserved RBS 1080..1091 /label=BBa_B0034 misc_feature 1098..1099 /label=prefix misc_feature 1271..1271 /label=T misc_feature 1589..1589 /label=A CDS 1098..1847 /label=luxR misc_feature 1098..1853 /label=BBa_C0062 misc_feature 1854..1878 /label=Help:Barcodes CDS 1098..1853 /label=BBa_C0062 stem_loop 1898..1941 /label=stem_loop misc_feature 1887..1966 /label=BBa_B0010 terminator 1887..1966 /label=BBa_B0010 stem_loop 1982..2001 /label=T7 TE misc_feature 2008..2008 /label=stop polyA_site 2002..2015 /label=polya misc_feature 1975..2015 /label=BBa_B0012 terminator 1975..2015 /label=BBa_B0012 misc_feature 1080..2015 /label=BBa_I0462 misc_feature 819..2015 /label=BBa_K1151014 misc_feature 2024..2043 /label=LuxR/HSL promoter 2043..2048 /label=-35 promoter 2065..2070 /label=-10 misc_feature 2076..2076 /label=start misc_feature 2024..2078 /label=BBa_R0062 promoter 2024..2078 /label=BBa_R0062 misc_feature 2091..2094 /label=conserved RBS 2087..2098 /label=BBa_B0034 misc_feature 2684..2715 /label=LVA CDS 2105..2683 /label=luxI misc_feature 2105..2722 /label=BBa_C0061 misc_feature 2723..2747 /label=Help:Barcodes CDS 2105..2722 /label=BBa_C0061 misc_feature 2087..2747 /label=BBa_C0261 misc_feature 2760..2763 /label=conserved RBS 2756..2767 /label=BBa_B0034 CDS 2774..2955 /label=BBa_K1151001 stem_loop 2975..3018 /label=stem_loop misc_feature 2964..3043 /label=BBa_B0010 terminator 2964..3043 /label=BBa_B0010 stem_loop 3059..3078 /label=T7 TE misc_feature 3085..3085 /label=stop polyA_site 3079..3092 /label=polya misc_feature 3052..3092 /label=BBa_B0012 terminator 3052..3092 /label=BBa_B0012 terminator 2964..3092 /label=BBa_B0015 misc_feature 2024..3092 /label=BBa_K1151015 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgg atacacccaa 241 taaagacgat tcaatcatcc gcttttcggt ttctttacaa caaaatttat tagacgaatt 301 agacaaccgc atcattaaaa acggctattc ttctcgctca gaattagtgc gcgacatgat 361 cagagaaaaa ttagtagaag acaattgggc cgaagataac cctaatgatg agagcaaaat 421 cgccgtgctt gtggtgattt atgatcacca ccaaagggaa ttgaaccagc gcatgataga 481 cattcagcat gccagcggga cgcatgtttt atgcaccact cacattcaca tggatgagca 541 taattgttta gagacgatta ttttacaagg caattcgttt gaaatccaac gcttgcaact 601 agaaattggg gggcttaggg gggttaaatt cgctaaattg actaaggcgt ctagctttga 661 acacaatgaa tagtactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact 721 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct 781 caccttcggg tgggcctttc tgcgtttata tactagagct cattctccta ttcttaaagt 841 gtttttcctt gaagacataa cgaaatcaag gttggatgta attgtagcaa tgttttgatt 901 tactaagatt aacagaagcg ttcattaact aattattatt taaaatgaat tagtgttata 961 tttttgaagc gctataaaag cgttattaga agcgcgttta ataccccttt agaatttata 1021 aagatcaaaa actcgcaaaa aaaatgagaa ttaaaattgg agtgataatg gtactagaga 1081 aagaggagaa atactagatg aaaaacataa atgccgacga cacatacaga ataattaata 1141 aaattaaagc ttgtagaagc aataatgata ttaatcaatg cttatctgat atgactaaaa 1201 tggtacattg tgaatattat ttactcgcga tcatttatcc tcattctatg gttaaatctg 1261 atatttcaat cctagataat taccctaaaa aatggaggca atattatgat gacgctaatt 1321 taataaaata tgatcctata gtagattatt ctaactccaa tcattcacca attaattgga 1381 atatatttga aaacaatgct gtaaataaaa aatctccaaa tgtaattaaa gaagcgaaaa 1441 catcaggtct tatcactggg tttagtttcc ctattcatac ggctaacaat ggcttcggaa 1501 tgcttagttt tgcacattca gaaaaagaca actatataga tagtttattt ttacatgcgt 1561 gtatgaacat accattaatt gttccttctc tagttgataa ttatcgaaaa ataaatatag 1621 caaataataa atcaaacaac gatttaacca aaagagaaaa agaatgttta gcgtgggcat 1681 gcgaaggaaa aagctcttgg gatatttcaa aaatattagg ttgcagtgag cgtactgtca 1741 ctttccattt aaccaatgcg caaatgaaac tcaatacaac aaaccgctgc caaagtattt 1801 ctaaagcaat tttaacagga gcaattgatt gcccatactt taaaaattaa taacactgat 1861 agtgctagtg tagatcacta ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa 1921 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac 1981 tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagacctgta ggatcgtaca 2041 ggtttacgca agaaaatggt ttgttatagt cgaataaata ctagagaaag aggagaaata 2101 ctagatgact ataatgataa aaaaatcgga ttttttggca attccatcgg aggagtataa 2161 aggtattcta agtcttcgtt atcaagtgtt taagcaaaga cttgagtggg acttagttgt 2221 agaaaataac cttgaatcag atgagtatga taactcaaat gcagaatata tttatgcttg 2281 tgatgatact gaaaatgtaa gtggatgctg gcgtttatta cctacaacag gtgattatat 2341 gctgaaaagt gtttttcctg aattgcttgg tcaacagagt gctcccaaag atcctaatat 2401 agtcgaatta agtcgttttg ctgtaggtaa aaatagctca aagataaata actctgctag 2461 tgaaattaca atgaaactat ttgaagctat atataaacac gctgttagtc aaggtattac 2521 agaatatgta acagtaacat caacagcaat agagcgattt ttaaagcgta ttaaagttcc 2581 ttgtcatcgt attggagaca aagaaattca tgtattaggt gatactaaat cggttgtatt 2641 gtctatgcct attaatgaac agtttaaaaa agcagtctta aatgctgcaa acgacgaaaa 2701 ctacgcttta gtagcttaat aactctgata gtgctagtgt agatctctac tagagaaaga 2761 ggagaaatac tagatggcac accatgaaga acaacacggc ggacaccacc accatcacca 2821 tcacaacacc accaccacta ccatggcggc gaacaccacc atcaccacca cagctctcat 2881 catgaagaag gttgttgtag cactagcgat agtcatcatc aagaagaagg ttgctgccac 2941 gggcatcacg agtaatacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga 3001 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg 3061 ctcaccttcg ggtgggcctt tctgcgttta ta //