LOCUS       BBa_K1151038            1950 bp    DNA     linear       14-Jan-2025
DEFINITION  Double generator NikR-GFP, IPTG-nickel regulated
ACCESSION   BBa_K1151038
VERSION     BBa_K1151038.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    1..220
                     /label=BBa_J04500
     CDS             227..673
                     /label=BBa_K1151000
     stem_loop       693..736
                     /label=stem_loop
     misc_feature    682..761
                     /label=BBa_B0010
     terminator      682..761
                     /label=BBa_B0010
     stem_loop       777..796
                     /label=T7 TE
     misc_feature    803..803
                     /label=stop
     polyA_site      797..810
                     /label=polya
     misc_feature    770..810
                     /label=BBa_B0012
     terminator      770..810
                     /label=BBa_B0012
     terminator      682..810
                     /label=BBa_B0015
     misc_feature    1..810
                     /label=BBa_K1151006
     promoter        819..1066
                     /label=BBa_K1151002
     RBS             1075..1087
                     /label=RBS-3\Weak
     RBS             1081..1084
                     /label=RBS
     misc_feature    1075..1087
                     /label=BBa_B0032
     RBS             1075..1087
                     /label=BBa_B0032
     CDS             1094..1813
                     /label=GFP protein
     CDS             1094..1813
                     /label=BBa_E0040
     stem_loop       1833..1876
                     /label=stem_loop
     misc_feature    1822..1901
                     /label=BBa_B0010
     terminator      1822..1901
                     /label=BBa_B0010
     stem_loop       1917..1936
                     /label=T7 TE
     misc_feature    1943..1943
                     /label=stop
     polyA_site      1937..1950
                     /label=polya
     misc_feature    1910..1950
                     /label=BBa_B0012
     terminator      1910..1950
                     /label=BBa_B0012
     misc_feature    1075..1950
                     /label=BBa_E0240
     misc_feature    819..1950
                     /label=BBa_K1151011
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgg atacacccaa
      241 taaagacgat tcaatcatcc gcttttcggt ttctttacaa caaaatttat tagacgaatt
      301 agacaaccgc atcattaaaa acggctattc ttctcgctca gaattagtgc gcgacatgat
      361 cagagaaaaa ttagtagaag acaattgggc cgaagataac cctaatgatg agagcaaaat
      421 cgccgtgctt gtggtgattt atgatcacca ccaaagggaa ttgaaccagc gcatgataga
      481 cattcagcat gccagcggga cgcatgtttt atgcaccact cacattcaca tggatgagca
      541 taattgttta gagacgatta ttttacaagg caattcgttt gaaatccaac gcttgcaact
      601 agaaattggg gggcttaggg gggttaaatt cgctaaattg actaaggcgt ctagctttga
      661 acacaatgaa tagtactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact
      721 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct
      781 caccttcggg tgggcctttc tgcgtttata tactagagtg agaaaaatcc ttttttggca
      841 tgagttcgtt aaaagccgct catgctagta tgattgaatt aatttaaaat taacaattat
      901 aatacaaact ggatttaaat ggttggccat aagagcgttt gaatttgatt gtaattatta
      961 gcttaatcat cattgacttg ttattattaa aacaatacaa tcaacaaacc aacattcttt
     1021 tatcattttg gagcatttat gcgaacggat gcttcttata gggtgctact agagtcacac
     1081 aggaaagtac tagatgcgta aaggagaaga acttttcact ggagttgtcc caattcttgt
     1141 tgaattagat ggtgatgtta atgggcacaa attttctgtc agtggagagg gtgaaggtga
     1201 tgcaacatac ggaaaactta cccttaaatt tatttgcact actggaaaac tacctgttcc
     1261 atggccaaca cttgtcacta ctttcggtta tggtgttcaa tgctttgcga gatacccaga
     1321 tcatatgaaa cagcatgact ttttcaagag tgccatgccc gaaggttatg tacaggaaag
     1381 aactatattt ttcaaagatg acgggaacta caagacacgt gctgaagtca agtttgaagg
     1441 tgataccctt gttaatagaa tcgagttaaa aggtattgat tttaaagaag atggaaacat
     1501 tcttggacac aaattggaat acaactataa ctcacacaat gtatacatca tggcagacaa
     1561 acaaaagaat ggaatcaaag ttaacttcaa aattagacac aacattgaag atggaagcgt
     1621 tcaactagca gaccattatc aacaaaatac tccaattggc gatggccctg tccttttacc
     1681 agacaaccat tacctgtcca cacaatctgc cctttcgaaa gatcccaacg aaaagagaga
     1741 ccacatggtc cttcttgagt ttgtaacagc tgctgggatt acacatggca tggatgaact
     1801 atacaaataa taatactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact
     1861 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct
     1921 caccttcggg tgggcctttc tgcgtttata
//