LOCUS BBa_K1152005 10611 bp DNA linear 22-Nov-2024 DEFINITION Expression cassette for NRPS synthesizing a Phe-Orn-Leu-Tripeptide ACCESSION BBa_K1152005 VERSION BBa_K1152005.1 FEATURES Location/Qualifiers promoter 138..143 /label=-35 promoter 162..167 /label=-10 misc_feature 174..174 /label=start CDS 228..3413 /label=TycAdCom CDS 3414..10442 /label=TycC5-TycC6 misc_feature 10577..10596 /label=T7 TE misc_feature 10603..10603 /label=stop ORIGIN 1 gcaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac 61 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact 121 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg 181 agcggataac aatttcacac atactagaga aagaggagaa atactagatg ttagcaaatc 241 aggccaatct catcgacaac aagcgggaac tggagcagca tgcgctagtt ccatatgcac 301 agggcaagtc gatccatcaa ttgttcgagg aacaagcaga ggcttttcca gaccgcgttg 361 ccatcgtttt tgaaaacagg cggctttcgt atcaggagtt gaacaggaaa gccaatcaac 421 tggcaagagc cttgctcgaa aaaggggtgc aaacagacag catcgtcggt gtgatgatgg 481 agaagtccat cgaaaatgtc atcgcgattc tggccgttct taaagcaggc ggagcctatg 541 tgcccatcga catcgaatat ccccgcgatc gcatccaata tattttgcag gatagtcaaa 601 cgaaaatcgt gcttacccaa aaaagcgtca gccagctcgt gcatgacgtc gggtacagcg 661 gagaggtagt tgtactcgac gaagaacagt tggacgctcg cgagactgcc aatctgcacc 721 agcccagcaa gcctacggat cttgcctatg tcatttacac ctcaggcacg acaggcaagc 781 caaaaggcac catgcttgaa cataaaggca tcgccaattt gcaatccttt ttccaaaatt 841 cgtttggcgt caccgagcaa gacaggatcg ggctttttgc cagcatgtcg ttcgacgcat 901 ccgtttggga aatgttcatg gctttgctgt ctggcgccag cctgtacatc ctttccaaac 961 agacgatcca tgatttcgct gcatttgaac actatttgag tgaaaatgaa ttgaccatca 1021 tcacactgcc gccgacttat ttgactcacc tcaccccaga gcgcatcacc tcgctacgca 1081 tcatgattac ggcaggatca gcttcctccg cacccttggt aaacaaatgg aaagacaaac 1141 tcaggtacat aaatgcatac ggcccgacgg aaacgagcat ttgcgcgacg atctgggaag 1201 ccccgtccaa tcagctctcc gtgcaatcgg ttccgatcgg caaaccgatt caaaatacac 1261 atatttatat cgtcaatgaa gacttgcagc tactgccgac tggcagcgaa ggcgaattgt 1321 gcatcggcgg agtcggcttg gcaagaggct attggaatcg gcccgacttg accgcagaaa 1381 aattcgtaga caatccgttc gtaccaggcg aaaaaatgta ccgcacaggt gacttggcca 1441 aatggctgac ggatggaacg atcgagtttc tcggcagaat cgaccatcag gtgaaaatca 1501 gaggtcatcg catcgagctt ggcgaaatcg agtctgtttt gttggcacat gaacacatca 1561 cagaggccgt ggtcattgcc agagaggatc aacacgcggg acagtatttg tgcgcctatt 1621 atatttcgca acaagaagca actcctgcgc agctcagaga ctacgccgcc cagaagcttc 1681 cggcttacat gctgccatct tatttcgtca agctggacaa aatgccgctt acgccaaatg 1741 acaagatcga ccgcaaagcg ttgcccgagc ctgatcttac ggcaaaccaa agccaggctg 1801 cctaccatcc tccgagaacc gagacagaat cgattctcgt ctccatctgg caaaacgttt 1861 tgggaattga aaagatcggg attcgcgata atttttactc gctcggcgga gattcgatcc 1921 aagcgatcca ggtcgtggct cgtctgcatt cctatcaatt gaagctagag acgaaagact 1981 tgctgaatta cccgacgatc gagcaggttg ctctttttgt caagagcacg acgagaaaaa 2041 gcgatcaggg catcatcgct ggaaacgtac cgcttacacc cattcagaag tggtttttcg 2101 ggaaaaactt tacgaataca ggccattgga accaatcgtc tgtgctctat cgcccggaag 2161 gctttgatcc taaagtcatc caaagtgtca tggacaaaat catcgaacac cacgacgcgc 2221 tccgcatggt ctatcagcac gaaaacggaa atgtcgttca gcacaaccgc ggcttgggtg 2281 gacaattata cgatttcttc tcttataatc tgaccgcgca accagacgtc cagcaggcga 2341 tcgaagcaga gacgcaacgt ctgcacagca gcatgaattt gcaggaagga cctctggtga 2401 aggttgcctt atttcagacg ttacatggcg atcatttgtt tctcgcaatt catcatttgg 2461 tcgtggatgg catttcctgg cgcattttgt ttgaagattt ggcaaccgga tacgcgcagg 2521 cacttgcagg gcaagcgatc agtctgcccg aaaaaacgga ttcttttcaa agctggtcac 2581 aatggttgca agaatatgcg aacgaggcgg atttgctgag cgagattccg tactgggaga 2641 gtctcgaatc gcaagcaaaa aatgtgtccc tgccgaaaga ctatgaagtg accgactgca 2701 aacaaaagag cgtgcgaaac atgcggatac ggctgcaccc ggaagagacc gagcagttgt 2761 tgaagcacgc caatcaggcc tatcaaacgg aaatcaacga tctgttgttg gcggcgctcg 2821 gcttggcttt tgcggagtgg agcaagcttg cgcaaatcgt cattcatttg gaggggcacg 2881 ggcgcgagga catcatcgaa caggcaaacg tggccagaac ggtcggatgg tttacgtcgc 2941 aatatccggt attgctcgac ttgaagcaaa ccgctccctt gtccgactat atcaagctca 3001 ccaaagagaa tatgcggaag attcctcgta aagggatcgg ttacgacatc ttgaagcatg 3061 tgacacttcc agaaaatcgc ggttccttat ccttccgcgt gcagccggaa gtgacgttca 3121 actacttggg acagtttgat gcggacatga gaacggaact gtttacccgc tcaccctaca 3181 gcggcggcaa cacgttaggc gcagatggca aaaacaatct gagtcctgag tcagaggtgt 3241 acaccgcttt gaatataacc ggattgattg aaggcggaga gctcgtcctc acattctctt 3301 acagctcgga gcagtatcgg gaagagtcca tccagcaatt gagccaaagt tatcaaaagc 3361 atctgcttgc catcatcgcg cattgcaccg agaaaaaaga agtagagcga acggcgcata 3421 ttgccgagag cgcattcgag cagttcgaga cgatccagcc agtcgagcct gccgcgtttt 3481 atcccgtgtc gtttgcccaa aagcgaatgt acatcctgca tcagttcgaa ggaagcggga 3541 tcagctacaa cgtgccgagt gtgctggtgc tggaaggcaa gctcgattat gaccgctttg 3601 ctgctgccat ccagagcctg gttaaacggc atgaatcttt gcgcacctcg ttccattcgg 3661 taaacgggga accgctgcaa cgagtacatc cggatgtcga gctgcctgtc cgccttttgg 3721 aggcgacaga agatcagagc gaatcgctca tccaggagct aatccagccg tttgatctgg 3781 agatagcccc gttgttcaga gtgaatctga tcaagcttgg cgcagagcgg cacttgttct 3841 tcatggatat gcaccacatt atttccgatg gcgtatcgct tgcggtcatc gtcgaggaaa 3901 ttgccagctt gtatgcagga aaacagcttt ccgacctgcg catccagtac aaagactttg 3961 ctgtgtggca gaccaagctg gctcagtcgg atcgcttcca aaaacaggag gatttttgga 4021 cccggacgtt tgccggggag attcctttgc tgaatctgcc ccatgattat ccaagacctt 4081 ctgtgcagag ctttgacggt gacacggtcg cgcttggcac cggacatcac ctgctggaac 4141 aactgcgcaa gctcgctgcc gagactggca cgaccttgtt catggtgctg ctggctgcct 4201 accatgtgtt gctctccaag tacgccggac aggaagaaat cgtcgtcggc acaccgatcg 4261 caggccgctc gcacgcagat gtcgagcgca ttgtcgggat gttcgtcaac acgctcgctt 4321 tgaaaaatac ggccgctggc agcctgagct tccgcgcctt tttggaagac gtgaagcaaa 4381 atgcgctcca tgccttcgag catcaagact atccgttcga gcatctggtc gagaagctgc 4441 aagtgcggcg cgatctgagc agaaacccgc tgtttgatac gatgttcagc ctggggcttg 4501 ccgaatcagc cgaaggagaa gtagcggatc tgaaagtgtc gccttatccg gtgaacggcc 4561 acatcgccaa attcgacctt tccctggatg cgatggaaaa acaggatgga cttcttgttc 4621 aattcagcta ttgcacgaag ctgttcgcaa aagaaacggt tgatcgactg gccgcccatt 4681 acgttcagct tttgcaaaca atcacagccg atcccgacat cgagctcgcc cggatcagcg 4741 tgttgtccaa agcagagacg gagcacatgc tgcacagctt cctcgcaacc aaaacagcct 4801 atccgacgga caaaacgttc cagaagctgt tcgaggagca agtggaaaaa acaccgaacg 4861 agattgccgt tctgttcggc aatgaacagc tgacctatca ggagttgaat gcaaaagcaa 4921 accagctcgc ccgcgtcctg cggcgaaaag gcgtcaagcc ggagagcacc gtcggcatcc 4981 tcgtagaccg ctcgctctac atggtcatcg gcatgctggc cgtgttgaaa gcaggcggaa 5041 cattcgtccc gattgatccg gactacccgc tggagcgcca agcgttcatg ctcgaagaca 5101 gcgaggcgaa gctgctgctc accttgcaaa aaatgaacag tcaagttgcc ttcccttatg 5161 aaacctttta tctggataca gagacagtgg atcaggagga gacgggcaat ctggagcacg 5221 ttgcgcagcc ggagaacgtc gcttacatca tctacacatc cggtacgacg ggcaagccaa 5281 aaggggtcgt catcgagcac cgcagctatg ccaatgtcgc atttgcctgg aaagacgaat 5341 atcacctgga cagcttcccg gtccgtttgc tgcaaatggc gagcttcgcc tttgacgtct 5401 cgacgggcga ttttgccagg gcgctgctga caggcgggca actggtcatc tgcccgaatg 5461 gggtcaaaat ggacccagct tcgctgtacg agaccatcag gcgtcacgaa attaccattt 5521 tcgaagcgac acccgccttg atcatgccgt tgatgcacta cgtttacgaa aacgaactgg 5581 atatgagcca aatgaagctg ctgattctcg gagcagacag ctgcccggcg gaagacttca 5641 aaacgttgct cgcgcgcttc ggtcagaaga tgcgcattat caacagctac ggcgtgacag 5701 aggcgtgcat tgacaccagc tactacgaag aaacagacgt caccgccatc cgctcgggaa 5761 cggtgccgat cggcaaaccg cttccgaaca tgacgatgta cgtggtcgat gcgcatttga 5821 atttgcagcc tgtcggcgtc gtaggcgaat tgtgcatcgg cggagcaggg gttgcgcgcg 5881 gttatttgaa cagacctgag ctgacggaag agaagttcgt gccgaatccg ttcgccccag 5941 gtgaacgatt gtaccgcaca ggtgatctgg cgaagtggcg cgcagatggc aatgtcgagt 6001 tcctcggacg caatgaccac caggtaaaaa tcaggggtgt ccgcatcgag ctgggcgaga 6061 tcgagacaca actgcgcaag ctggacggaa ttacggaagc agtcgtggtt gcgagagaag 6121 atcgcgggca ggaaaaggaa ttgtgcgcat acgtcgtggc ggaccacaag cttgacaccg 6181 cagaattgcg ggcgaatttg ctgaaggaac tgccgcaagc gatgattcca gcgtatttcg 6241 tcaccttgga tgcgctgccg ctgactgcca atggcaaagt agacagacgt tccttgccag 6301 cgccggatgt caccatgctg agaacgaccg agtatgtagc gccgcgctcc gtctgggaag 6361 cccgattggc ccaagtatgg gagcaggtgc tgaatgttcc gcaagtgggt gcgctagacg 6421 actttttcgc gctcggcggt cactcattgc gtgccatgcg cgtcctttcc agcatgcaca 6481 acgaatacca ggtcgacatc ccgctgcgca tcttgttcga aaaaccgacg attcaggaac 6541 tggcggcgtt catcgaagag acagccaaag ggaatgtctt ctcgatcgag cctgtgcaaa 6601 agcaagcgta ctatccggtc tcctcggcac aaaagcgcat gtacatcctc gatcaatttg 6661 agggagtcgg catcagctac aacatgccgt cgactatgct gatcgaaggc aagctggagc 6721 gaacacgggt agaagcggcg ttccagcgct tgattgcgcg acatgaaagc ctgcgcactt 6781 cgtttgccgt cgtcaacgga gagcctgtgc aaaacattca cgaggacgtt ccgtttgcgc 6841 ttgcctattc ggaagtcaca gaacaggagg cgcgcgaact cgtttcttct ctcgtgcagc 6901 cgttcgatct ggaggtcgca ccactcatcc gcgtgtcgct gctgaaaatc ggcgaggatc 6961 gttacgtgct ctttaccgac atgcatcaca gcatttccga tggcgtatcc tccggcattc 7021 ttttggcaga gtgggtgcag ctgtaccagg gtgacgtttt gccggagctg cgtatccagt 7081 acaaggactt tgctgtgtgg caacaagagt tttcccagtc ggctgccttc cacaagcagg 7141 aagcgtactg gttgcaaacg tttgccgatg acattcctgt gctgaacttg ccgaccgatt 7201 tcacccgccc cagcacccaa agctttgccg gggatcagtg cacgatcggc gcgggcaaag 7261 cgctcacgga aggcttgcac cagttggcgc aggcgacggg aacgactttg tacatggttt 7321 tgctcgccgc gtacaacgtg ctgctcgcca agtatgccgg gcaggaggac atcatcgtcg 7381 gcacgccgat tacaggcaga tcccatgccg atctcgaacc gatcgtcggc atgttcgtga 7441 acaccttggc gatgcgaaac aaaccgcagc gcgaaaagac ttttagcgag tttttgcaag 7501 aagtcaagca aaatgcgctg gatgcgtacg gccatcagga ttacccgttt gaagaactgg 7561 tggaaaagct cgcgatcgcg cgcgatttga gccgaaatcc gctgtttgac accgtgttta 7621 cgttccaaaa cagcacggaa gaggtcatga cgctgcctga atgcacgctt gcgccgttta 7681 tgacggacga aacaggccag cacgccaagt tcgacttgac tttcagcgct acggaagagc 7741 gggaagaaat gacgattggc gtggagtaca gcacaagctt gtttacgcgg gaaacgatgg 7801 aacggttcag ccgccacttc ctgacgattg cagcgagcat cgtgcaaaat ccgcacatcc 7861 gtctgggcga gatcgacatg cttttgccag aagaaaaaca gcagattttg gccgggttca 7921 acgatacggc agtcagctat gcgctggaca aaacgctgca ccagctattc gaagagcagg 7981 tcgacaaaac accggatcag gcagcgcttc tctttagcga gcaatcgctg acgtacagcg 8041 aactgaacga gcgagcaaac agactggcaa gggtcctgcg cgcaaaagga gtcggaccgg 8101 accgtctggt agcgatcatg gcggagcgct cgccggaaat ggtgatcggt attctcggta 8161 ttttgaaggc aggcggcgct tatgttcccg tcgatcccgg ctatccgcag gagcgcattc 8221 agtacctgct cgaagatagc aacgcagccc tgctgctcag ccaggcgcat ctgttgccgc 8281 tgttggccca ggtgtcaagc gagctgccgg agtgccttga tctgaacgct gaactggatg 8341 ccggactgag cggctccaac ctgccagctg tcaaccaacc gactgacctt gcctacgtca 8401 tctatacatc cggtacgacc ggcaagccga agggtgtcat gatcccgcat caaggaatcg 8461 tgaactgctt gcagtggaga agagacgaat acgggttcgg gccgagtgac aaggcgttgc 8521 aagtgttctc ctttgccttc gacggttttg tagccagctt gttcgctccg ctgctcggag 8581 gggcaacgtg cgtgttgccg caagaagcag ctgccaaaga cccggtcgcg ctgaaaaaac 8641 tgatggccgc aacggaagtc acccattact acggcgtacc gagtctgttc caggccattc 8701 tcgattgctc gacgacaacc gacttcaatc agttgcgttg cgtcactttg ggcggcgaga 8761 agctgcctgt gcagcttgtg caaaaaacaa aagaaaagca tccggcaatc gagatcaaca 8821 acgagtacgg cccgacggaa aacagcgtcg tcaccaccat ctcgcgctcg attgaagcgg 8881 ggcaagcgat cacgattggc cgaccgcttg cgaacgtcca agtctacatt gtagatgagc 8941 agcatcactt gcagccgatt ggcgtggtcg gtgagctgtg catcggcgga gccgggcttg 9001 ccagaggcta tctgaacaaa ccggagctga ccgcagagaa gtttgtcgca aatccgttcc 9061 gaccaggcga gcgcatgtac aaaacaggcg acttggtaaa atggcggacg gatggcacga 9121 tcgagtacat cggccgcgca gacgaacagg tcaaggtgag agggtatcgc atcgagatcg 9181 gcgagatcga gagcgccgta ctcgcttacc agggcatcga tcaagcggtg gtcgttgcgc 9241 gagacgatga cgctacggct ggttcctatc tttgcgccta ctttgtcgca gcaacagccg 9301 tgtccgtatc cggcttgaga agccatctgg ccaaagagct gcctgcttac atgattccga 9361 gctatttcgt cgagctggat cagctgccgc tttccgccaa tggaaaagtg gatcgcaaag 9421 ctttgccgaa gccgcaacag tccgatgcga ccacgcgcga atacgtggcc ccgaggaatg 9481 cgaccgaaca gcaactggca gccatctggc aagaagtttt gggagtagag ccaatcggca 9541 tcaccgacca gttctttgaa ctcggaggac attccttaaa agctacgctg ttgattgcca 9601 aagtgtatga gtacatgcaa atcgagctgc cgctgaatct catcttccag tatccgacga 9661 tcgaaaaggt ggccgatttc atcacgcata agcgctttga gagcagatac ggcacagcca 9721 ttttgttaaa tcaggagacg gcgcgaaacg tattttgctt cacgccgatc ggcgcacaaa 9781 gcgtgtacta ccagaagctt gcggcggaaa ttcaaggcgt ctctttgtac agctttgatt 9841 tcatccagga tgacaaccgg atggagcagt atatcgcggc gatcaccgca attgatccaa 9901 gcggtccgta cacgctcatg ggctactcct cgggaggcaa tctggctttt gaagtggcga 9961 aagaactgga ggagcggggc tatggcgtca ccgacatcat cttgttcgac tcgtactgga 10021 aagacaaggc gattgagcgg actgtcgcgg aaacagaaaa cgacattgcc cagctattcg 10081 ccgagattgg cgaaaacacc gagatgttca acatgacgca agaagacttc cagctgtacg 10141 ccgccaatga gtttgtcaag caaagcttcg ttcgcaaaac ggtcagctat gtgatgttcc 10201 ataacaatct ggtcaatacc ggaatgacca ctgccgcgat ccacctcatc caatccgagc 10261 tggaagcaga cgaggaagct ccggtggcag ccaagtggaa cgaatcagcc tgggcaaacg 10321 caacgcaacg actgctgacg tacagcgggc acggaatcca ctcgcgcatg ctggcgggcg 10381 attacgcgtc gcaaaatgct tcgattttgc aaaacatcct gcaagaactg ttcatcctga 10441 aataataacg ctgatagtgc tagtgtagat cgctactaga gccaggcatc aaataaaacg 10501 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct 10561 ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata t //