LOCUS BBa_K1152006 6903 bp DNA linear 14-Nov-2024 DEFINITION Valine-Indigoidine fusion - NRPS ACCESSION BBa_K1152006 VERSION BBa_K1152006.1 FEATURES Location/Qualifiers promoter 138..143 /label=-35 promoter 162..167 /label=-10 misc_feature 174..174 /label=start CDS 228..1958 /label=TycC4dC misc_feature 1959..2879 /label=C(TycC2) misc_feature 2880..6734 /label=indC misc_feature 6869..6888 /label=T7 TE misc_feature 6895..6895 /label=stop ORIGIN 1 gcaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac 61 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact 121 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg 181 agcggataac aatttcacac atactagaga aagaggagaa atactagatg tatccgcgcg 241 atctgacgat tcaggagctg ttcgagcagc aggcagcaaa aactccggag catgcagcgg 301 tcgtgatgga cggacagatg ctgacgtatc gggagctgaa cgaaaaagcg aaccagctcg 361 cccatgtcct tcgtcaaaac ggagtcggga aagagagcat cgtcggtctg ctcgcagatc 421 gttcgctgga aatgattaca ggcatcatgg ggattctcaa agcgggcggc gcctacctgg 481 gactggaccc ggagcatccg tccgaacgcc tggcttacat gttggaagat ggcggcgtga 541 aagttgtcct cgtgcaaaag cacttgctgc cgctcgtcgg cgaagggctg atgccaatcg 601 ttttggaaga ggagagcctg cgcccggaag attgcggcaa tccggcgatt gtcaacggtg 661 cgagtgacct ggcttatgtg atgtacacct caggctctac aggcaagcca aaaggagtca 721 tggtcgagca tcgcaacgtc acccgcttgg tcatgcatac gaattacgtg caagtgcgcg 781 agagcgaccg gatgattcaa accggcgcga ttggcttcga cgccatgaca tttgagattt 841 ttggagcctt gctgcacggg gccagcctgt atttggtgag caaggacgtc ttgctggatg 901 ccgaaaagct gggcgacttc ctgcggacga atcagattac gaccatgtgg ctgacctcgc 961 cgctcttcaa ccagctttcg caagacaatc cggcgatgtt tgacagcttg cgcgccttga 1021 tcgtcggtgg cgaagcgttg tcgccgaagc acatcaaccg ggtaaaaagt gcccttcctg 1081 acctggaaat ctggaacgga tacggcccga ccgaaaacac gaccttctcg acgtgctatt 1141 tgattgagca gcattttgaa gagcagattc cgatcggcaa gccgattgca aactccaccg 1201 cgtatatcgt cgacggcaac aatcagccgc agccgatcgg cgtaccgggt gaactgtgcg 1261 tcggtggtga cggtgtcgca agaggctatg tgaacaagcc ggaattaacc gccgaaaagt 1321 ttgtgcccaa tccgtttgcg cctggcgaaa cgatgtatcg caccggagat ttggcgagat 1381 ggctgccgga tgggacgatt gagtatttgg gccgaatcga ccagcaggtc aaaatcaggg 1441 gataccggat cgagcttggg gaaatcgaga cggtcttgtc ccagcaggca caagtaaaag 1501 aagcagtcgt ggccgtgatc gaggaggcga acgggcaaaa agctctctgc gcttactttg 1561 tgccagaaca ggccgtcgac gccgcagagc tgcgagaagc gatgtccaaa caattgcctg 1621 gctacatggt ccctgcttac tatgtgcaaa tggaaaagct gccgttgacc gcgaacggaa 1681 aggtcgaccg ccgggcattg ccgcagccat ccggcgagcg gacgacagga agcgcctttg 1741 tcgctgcgca aaatgatacc gaagcgaagc tgcaacagat ttggcaagaa gttttgggca 1801 ttccggcaat cggcattcac gacaacttct ttgaaatcgg cggtcattcc ttgaaggcga 1861 tgaacgtcat cacgcaagtc cataaaacat tccaggtgga gctgccgtta aaagcgctgt 1921 ttgccactcc gacgatccat gagttggctg cgcatatttc ggaaaaaacc gagtacaccg 1981 cgattcaacc cgtggcagcg caggagtttt acccggtttc atctgcgcaa aaaagaatgt 2041 atatcctgca acagttcgaa ggcaacggaa tcagctacaa catttcgggt gcgattctcc 2101 tggaaggaaa gctggactac gcccggtttg ccagcgctgt gcaacagctg gcagagcgcc 2161 acgaagcttt gcgcacctcg ttccaccgga tcgacggcga gcctgtgcaa aaagtgcacg 2221 aggaagtaga agtgccgctt ttcatgctgg aggctcccga agaccaggcg gagaaaatca 2281 tgcgcgagtt tgtccgtccg tttgatctcg gggtcgctcc gctgatgcga acaggtttgc 2341 tcaagctggg caaagaccgc catttgtttt tgctcgacat gcaccatatc atctcggacg 2401 gcgtttcttc gcaaattttg ctgcgtgaat ttgccgagtt gtaccaggga gcagacttgc 2461 agccgctttc gctgcaatac aaagatttcg ctgcttggca aaatgagctg tttcagacgg 2521 aggcatacaa gaagcaggag cagcactggc tgaacacgtt tgctgatgaa attccgctct 2581 tgaacctgcc gactgactat ccgcgcccta gcgtgcaaag ctttgcaggc gatctcgtcc 2641 tttttgccgc cggaaaagaa ctgctggagc ggttgcaaca ggtagcgtca gaaacaggca 2701 ccaccttgta catgattttg cttgccgcct acaatgtgct gctgtccaag tataccggcc 2761 aggaagacat catcgtcggg acgcctgtcg ctggacgttc ccatgcggac gtggaaaaca 2821 tcatgggcat attcgtgaac acattggcgc tgcgcaacca gcctgccagc agcaaaacga 2881 tgttagaaaa taatattaca caatgtgact caatcaatga tgtttatctt aaagaagaag 2941 caataacatt gatggatatg cttgagagtc aacttaagca ccaggcagat ggatatgttg 3001 ttattgatca agaagaatct ctcagttacg ctgatttcta tttgagggtg aaagagatag 3061 ggtattgtct gtcagaaatt agctcaaaga gttcggtggg tattgggctt ttttgtgatc 3121 cttctataga tttaatttgt ggtgcatggg gtattttgtc agcggataaa gcttatttgc 3181 cgttatcgcc tgactatcca actgaacgcc tcaaatatat gatagaagat tctggtattg 3241 atgtgatttt tacgcaatcg cacttaaaag cacagctaca ggacattgca ccaaaatcag 3301 tattaattat gacaccagaa gatgtcgctc tgacgataaa aacacgaaca atagaagata 3361 ttctgggcac agttcaagtt cctaaaccca cgagtctggc ttatattatt tatacctctg 3421 gtagcacggg taagccaaag ggagtgatga ttgaacatca cagtattgta aatcaaatga 3481 gatttcttgc aaaagcgttc aaattaggat gtcattcccg gattttacag aaaacaccaa 3541 tgagttttga tgcggctcaa tgggaaattc tagcgcctgc aattggtggt caagtgatta 3601 tgggtccttt aggttgctat cgcgatccgg atgcaattat taaaaccatt cttcagcatc 3661 aagtaacgac tttgcaatgt gttcctactt tgctacaagc gttactggat aatcctaatt 3721 ttttggattg cttatcattg actcaagtat tcagtggggg agaagcgctg acaaccaaat 3781 tagccacgca atttttgaat agttttactc actgtgaatt aatcaattta tatggcccga 3841 cagaatgtac gattaattca tcatttttcc gggtgacaaa tgagactttg ccgaattatc 3901 aaacctctat ttcgattggt gcacctgtag ataataccga atactacgtt cttgatgatg 3961 atagattacc tgtggcggtt ggcgaaattg gcgagcttta tatttcgggt gctcaattag 4021 cacgtggtta tttgcataaa ccagaaatga caaaagataa atttatttgt aatcaccttg 4081 tatcaggaac tcaacatcaa tggttatatc gaacgggaga tctggtaacc agaggggctg 4141 atggtaatac ttattttgtt ggtcgggttg atagccaggt caaattacga ggttaccgta 4201 ttgagcttga tgaaatacgc catgcgattg aagaacatag ctggataaag acggcggcaa 4261 tgttaattaa gaaggatgcc agaacgggtt tccaaaatct catcgcgtgt gtggaattag 4321 atgagaaaga agctgcattg atggatcaag gtaatagtag ctcacatcac aaatcaaaag 4381 ccgataaact acaggtgaaa gcccaacttt ctaattctgg ttgtcgaagt gaagagttat 4441 gtgaaaatcg ccctacattc ttacttcctt atcaagaagg ggagataaaa cagagagaat 4501 atgcatttgg acgcaagaca tatcgctatt ttgagggaac agaaataacg gtagagaaat 4561 taaaaaaatt gctgacagcc actcaatcga atgaaattag ctctttgcca ctgagtcatc 4621 taaccctgaa tgatttcggt tatgcattgc gttattttgg tcagtttacc agccatcaac 4681 gtttattgcc caaatatgcc tatgcttcac cgggtgctct ctatgcgaca caaatgtatt 4741 ttgaattgca taatgttctc ggtttggatg cggggattta ctattatcat ccagtgacac 4801 ataagttaat aaaaatttca acattgagtc gtcggcaaat gccaacgata aaagtgcatt 4861 ttattggcaa gcatgaagcc attgagcccg tttataagaa caatatacaa gaagttctgg 4921 aaatggaagc gggccatatg atgggtcttt ttgatgacgt attaccggaa attggcttga 4981 gtattggtaa aagtgaatat caagatgaat gtccagattg gtatgatggt gatattcagg 5041 attattatct tggtgcattt gaaatatgta gctatgaaca tggattgccg ccatttgaga 5101 ctgatattta tttacaaaca catgcccata aaatacctga gatgccgtgt ggtttatatc 5161 acttttctaa cggggaattt gtacgaataa gtgatgatat tgtccgaaaa aaggatgtta 5221 ttgcgattaa tcagcaagtt tatgatcgct ccagttttgg cgtgtcaatt attccacgct 5281 gtgtccctga atggcattat tatataacac tgggtcgtcg gttacatgcg ttacaaagta 5341 atccattgta tattggatta atgtcatctg gttacagttc gaagagcaat aacgatttac 5401 cttcggcgaa aaggatgcga tctattctca atgcacttga tagacctatg gcggcatttt 5461 atttctgcat aggtgggggt attagccaag cgcaatatat gtgtgaaggc atgaaagaag 5521 atgttgttca tatgaaaggg ccagttgaaa tcattaaaga tgatcttcaa caacaactcc 5581 ctcaatatat gattccaaat aaggtattag ttttcgataa attacctttg acggccaatg 5641 gaaaagtgga ttatcaatct ttatcagaat ctaaagccgt ggagaatgtt tcaacacagc 5701 gtctattggt gccattacat acagatactg aaataaggct tggaaaaatt tggatggaag 5761 tactgaaatg ggattcagta tctgccctcg atgatttttt cgaaagtggg ggtaattctt 5821 tgatggccgt tgcaatggtt aataagatca atgcggcctt taatattcgt tttccgttac 5881 agatactttt tcaatctcct aatatagcag aattggctaa gtggattgaa cagacagact 5941 ctaaaacaat atcaagatta attttattga atcaggcaag caaagacccc atttactgtt 6001 ggccgggttt gggcggatat cctatgagtt tgagattgct tgctaataaa gtcgttcctg 6061 atcgggcatt ttatggaata caggcatatg ggataaacga gagtgaaata ccgttttctt 6121 ctatccagag aatggcagaa gaggatatta aagagataaa gaaaatacag ccagaagggc 6181 catatatatt gtggggatat tcatttggtg cccgagtagc atttgaagtt gcataccagc 6241 ttgaacaagc gggagaagaa gttaacgcat tgaatttatt ggctccggga tctcctcatc 6301 ttgatatgaa gcaagcggaa tatatggata aaggcgctga atttactaat ccggcttttg 6361 ttaaaatact tttttctgta ttttctcgtt caatcaacag cccaatggtt aaaacttgct 6421 tagaacaagt aaatagtgaa acgacattta ttaactttat atgtagtcgt tttaaaaact 6481 tggaaccatc attagtaaaa cgtatcgtta ggattgtgac tttgacttat gatttcaagt 6541 acagtattga tgagctttat cacagacacc taaaggcacc tataactatt ttcaaggcga 6601 atagagataa tgattcattt atcgaggaat cggatgtgat ttcatcaatg tcgcctaaaa 6661 taattgaatt aatatcggat cactatcaac tgttggaaag tgaaggtgtt gctgagattg 6721 agaaaataat ctaataataa cgctgatagt gctagtgtag atcgctacta gagccaggca 6781 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc 6841 ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta 6901 tat //